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Biomedical subjects

K Bauer

Publications and source records attributed to K Bauer.

At least 289 records · Page 16Linked to original sources
PubMed1975

Subcellular distribution of phosphatases, proteinases, and ribonucleases in normal human stratum corneum and psoriatic scales.

The subcellular distribution of phosphatases, proteinases, and ribonucleases of normal human stratum corneum and psoriatic scales was determined after differential centrifugation. All psoriatic enzymes showed much increased activities as compared to the normal stratum corneum enzymes. The highest activities of alkaline phosphatase from psoriatic scales could be detected in the nuclear fraction. The main activities of all other tested phosphatases and proteinases were present in the cytoplasmatic fraction. The subcellular distribution of the ribonucleases varied according to the pH value.

Alkaline Phosphatase↗
PubMed1975

The relationship between histones F 2al and F 2a2 and the ancestral histone A peptide. Further evidence for the common origin of histones F 2al, F 2a2 and F 3.

The relationship between histones F 2al and F 2a2 becomes much more apparent if the alignment is not made between the total sequences but between the ancestral A peptide, reconstructed earlier for histone F 2al (IV) and F 2a2. 46.5% of the latter's sequence can thus be clearly connected with F 2al through this ancestral dodecapeptide. A parallel development of histones F 2al, F 2a2 and F 3 from the A peptide is proposed.

Amino Acid Sequence↗
PubMed1975

Affinity-labelling of Escherichia coli ribosomes by a derivative of phenylalanyl-tRNA. A critical test for the specificity.

Under nonenzymatic binding conditions p-nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p-nitrophenoxycarbonyl-phenylalanyl-tRNA.

Affinity Labels↗
PubMed1975

Tyrocidine and the linear gramicidin. Do these peptide antibiotics play an antagonistic regulative role in sporulation?

1. The cyclic peptide antibiotic tyrocidine, synthesized by Bacillus brevis (ATCC 8185), inhibits RNA synthesis in an in vitro transcriptional system by forming a complex with the DNA. 2. The linear peptide antibiotic gramicidin, synthesized by the same strain, reverses at least partly this inhibition. The molecular mechanism of this reactivation is unknown. Gramicidin by itself inhibits transcription in vitro. This inhibition is not due to a complex formation between DNA and the peptide. 4. A possible regulative role of the two peptides in sporulation is discussed.

Bacillus↗
PubMed1975

Some investigations on the adjuvant mechanism of DEAE dextran.

In vitro it was shown that adsorption of inactivated FMDV onto DEAE-D kieselgur columns did not occur in the presence of 0.1--0.15M NaCl. These NaCl concentrations are present in DEAE-D/FMDV vaccines and in the tissues of animals. Therefore, adsorption of virus antigen does not appear to be responsible for the adjuvant effect of DEAE-D. In pigs it was demonstrated that DEAE-D exerts its optimal adjuvant effect, as measured by the formation of neutralizing antibodies and protection against challenge infection, when injected together with inactivated FMDV as vaccine. Apart from this, a good adjuvant effect (group immunity 75--100 per cent) was evoked in about one half and a moderate effect (group immunity 50--70 per cent) in about a quarter of the inoculated animals even if DEAE-D was separately injected locally and temporally from the inactivated virus. With regard to immunity it apparently does not matter whether DEAE-D or inactivated virus was given first, but an interval of 48 hours or 4 days between injection seemed to be more favourable than one of 24 hours. With regard to the formation of neutralizing antibodies the situation is comparable to that of immunity with the exception that a time interval of 24 hours between the application of DEAE-D and inactivated virus or vice versa was as good as that of 48 hours or 4 days. The results are discussed in regard to the possible mechanism of the adjuvant effect of DEAE-D on the cellular level.

Adjuvants, Immunologic↗
PubMed1975

[Studies on the Relationship between immunity and the level of neutralizing antibodies in pigs vaccinated against foot-and-mouth disease (author's transl)].

The statistical relationship between the titers of neutralizing antibodies and the immunity of 706 pigs vaccinated against FMD was studied. This was done for each of four virus strains separately. Whereas no correlation between both test systems could be detected in case of the strains A5 Westerwald, C Detmold and O1Santander, a significant correlation was ascertained for the strain O1Kaufbeuren. Becuase of the different findings depending on the virus strain under study it was concluded that the antibody titer alone does not provide a useful measure for potency testing of FMD-vaccines for pigs.

Animals↗
PubMed1975

[Studies on the influence of the host system on the result of the neutralization of monodispersed and untreated foot- and mouth disease virus (author's transl)].

Monodispersed foot- and mouth disease virus was obtained by filtration through membranes having a porosity only twice the diameter of the virus. This monodispersed virus was neutralized by immune sera in the same manner as not treated virus. This was shown by the inactivation curve comparing the amount of virus used for the test (calculated as ID50) with the neutralization titre (ND50) or comparing the reduction of infectivity (also calculated as ID50) with the dilution factor of the antiserum. When the virus was assayed by counting the plaque forming units, the monodispersed virus needed more antiserum to be neutralized than a not treated virus suspension. The same mixtures of virus and antiserum were inoculated into different host systems. Using suckling mice there was observed only about 1% of the infectivity measured in primary calf kidney cells. Primary calf kidney cells showed only a tenth of the infectivity seen in BHK-21 cells. The difference between the results in mice and calf kidney cells has been found to be caused by the existence of infectious virus in the mice, which does not cause illness or death in the presence of antibodies. Comparing the same size of cell sheet there is more virus absorbed by BHK-cells in the presence of antibodies than by calf kidney cells. The different results obtained in different hosts for the same virus antibody mixtures are discussed with respect to the detection of small amounts of antibodies and to the limits for the comparison of neutralization titres.

Animals↗