Isolation of Japanese encephalitis virus from mosquitoes collected in Bankura district (West Bengal) during October 1974 to December 1975.
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Biomedical subjects
Publications and source records attributed to K Banerjee.
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Larvae of Rhipicephalus haemaphysaloides were infected with Kaysanur Forest disease (KFD) virus by feeding on viraemic rodents and reared into next generation larvae. Fed larvae, nymphs, unfed adults, fed adult males, and females after oviposition were found infected, while the larvae were found free from infection. Nymphs and adults transmitted the infection by bite to rodents and rabbits respectively. The virus was also passed through a second rodent-tick cycle. Adult ticks showed a titre of 3.1 to 4.5 dex mouse LD50/0.03 ml, and the virus was also detected 245 days after infection.
Adult Swiss albino mice were immunized intravenously with flaviviviruses. Cellular immune response was studied by macrophage migration inhibition assay. Migration of cells from sensitized mice was significantly inhibited in the presence of homologous virus or antigen. Migration inhibition was also significant with serologically related viruses, thus establishing cross reaction in cellular immune response.
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This study measures the airborne radioactivity during the handling of millicurie quantities of I-131 in the liquid and capsule form. The data indicate there is significant airborne activity when bottles containing 100--145 mCi of liquid I-131 are opened, and that the Nuclear Regulatory Commission level for airborne activity of I-131 in restricted areas (9 X 10(-9) muCi/ml) is exceeded. However, the airborne activity of I-131 is below the Nuclear Regulatory Commission level for restricted areas when 100-mCi quantities of I-131 in the capsule form are used, or during handling of liquid I-131 in the 20- to 30-mCi range. Thyroid counting is a better method than film badge for monitoring the personnel.
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Progressive reduction in blood cell counts was observed in mice inoculated intracerebrally (ic) with Japanese encephalitis (JE) virus. No changes were observed in the blood cell counts of mice inoculated intraperitoneally (ip). Reduction in cell counts after a transient rise was noticed in lymph nodes of mice inoculated by either route but the cell counts returned to normal in lymph nodes of ip inoculated mice by the 8th day post inoculation (p.i.). JE virus antigen was demonstrated by immunofluorescence in mononuclear cells from the blood, spleen and lymph nodes starting from the 3rd and 4th day p.i. in mice inoculated ic and ip, respectively. The number of fluorescent cells increased as the infection progressed. The number of fluorescent spleen cells uas higher in ip than in ic inoculated mice. Live virus could only occasionally be demonstrated in the cells.
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