Antibodies in gastric juice.
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Anemia, Pernicious↗
Biomedical subjects
K B Taylor
Publications and source records attributed to K B Taylor.
Autoimmunity in pernicious anemia and thyroiditis: a family study.
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Anemia, Pernicious↗
The production of recombinant beta-galactosidase in Escherichia coli in yeast extract enriched medium.
The productivity of Escherichia coli biomass and recombinant beta-galactosidase was increased in Luria broth (LB) enriched with yeast extract. In flask culture under conditions of LB limitation, yeast extract supplementation gave the highest biomass (strain HB101/pRW756) stimulation per unit of component added compared with supplementation by various amounts of amino acids, vitamins, minerals, purines/pyrimidines, tryptone, casamino acids, casein peptone or gelatin peptone. The biomass production of E. coli HB101/pRW756, XL-1 Blue/puc118, XL-1 Blue FF/puc118 and TB-1/p1034 cells was stimulated in fermentor-scale experiments with additional yeast extract in LB. Total beta-galactosidase production from plasmid genes in fermentor-scale experiments was increased 105.4% in XL-1 blue/puc118 cells, 365.5% in XL-1 blue FF/puc118 cells and 421.4% in TB-1/p1034 cells by 0.5%, 1% and 1% weight per volume of additional yeast extract in LB, respectively. Depending on different strains, the increase of the enzyme production was obtained either by increased biomass, or the combination of enhanced gene expression and increased biomass. Neither the biomass nor beta-galactosidase production was stimulated in N4830/p1034 cells by the increase in yeast extract concentration in the medium.
Culture Media↗
Effect of glucose on the expression parameters of recombinant protein in Escherichia coli during batch growth in complex medium.
The expression parameters were determined at different phases of batch growth of Escherichia coli JM101/pYEJ001 in complex medium and at different conditions of glucose addition. Thus the plasmid content, the RNA content, the RNA synthesis rate, the specific recombinant mRNA content, the specific recombinant mRNA synthesis rate, the recombinant chloramphenicol acetyltransferase content, and the overall protein synthesis rate were determined during growth with no glucose, with initial glucose, with glucose feeding during stationary phase, and with initial glucose plus glucose feeding during stationary phase. The results show that the specific rate of total RNA synthesis was enhanced in the presence of glucose at both exponential and stationary phases, while recombinant mRNA synthesis was enhanced only at stationary phase by glucose feeding. However, the steady-state level of the recombinant mRNA was not changed under the same conditions. In addition, although the overall protein synthesis rate at exponential phase was enhanced in the presence of glucose, the specific recombinant protein level was unaffected. The specific synthesis rate of recombinant mRNA varied inversely with the plasmid content during exponential and stationary phases. Furthermore, changes in the specific activity of the recombinant protein were not correlated with either the changes in the specific synthesis rate of the recombinant mRNA or the overall protein synthesis rate. Therefore, the specific activity of the recombinant protein is not universally limited by its gene transcription rate or the overall protein synthesis capacity.
Chloramphenicol O-Acetyltransferase↗