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Biomedical subjects

K Asanuma

Publications and source records attributed to K Asanuma.

At least 73 records · Page 4Linked to original sources
PubMed1994

Biological characteristics of a continuous cultured cell line derived from a human malignant fibrous histiocytoma.

A continuous cultured cell line, NATO, was established from a human malignant histiocytoma of bone. The cultured cells consisted of at least 3 types of tumor cells; polygonal, long spindle and occasionally giant cells, which were primarily observed in the original tumor of the patient. Ultrastructurally, they were mostly immature cells that had poorly developed cell organelles and a few lysozomal granules. The cultured cells had phagocytotic activity and were positive for acid phosphatase, transferrin receptor, alpha 1-antitrypsin, alpha-naphthyl acetate esterase and HLA-DR, but negative for Fc- and C3-receptors. Allotransplantation of the cells into athymic nude mice produced tumors at early passages, but did not 3 years later. Four cloned sublines isolated from the parent line also showed essentially an identical morphology with that of the parent cell line, indicating that the 3 cell types were interchangeable.

Animals↗
PubMed1994

Significance of values of thyrotropin binding inhibitor immunoglobulins and appearance of intrathyroidal lymphocytes at subtotal thyroidectomy for Graves' disease.

This study was undertaken to investigate whether thyrotropin binding inhibitor immunoglobulins (TBII) values at and after subtotal thyroidectomy correlated with the outcome or the histologic grade applied to the appearance of intrathyroidal lymphocytes at the time of surgical treatment. In addition, the main reason for the study was to determine whether or not it was possible to predict the outcome after the operation by the data obtained. There was no relation between the TBII results and the grade of the appearance of intrathyroidal lymphocytes at the time of operation or the TBII postoperative results (whether positive or negative) and the final outcome. However, it was of interest that patients with a recurrence of hyperthyroidism had the TBII values of more than 50 percent at the time of surgical treatment, and also manifested continuously positive TBII values after the operation. They also had moderate grades of lymphocytic infiltration and lymph follicle formation in the surgical specimen. It seemed impossible to predict the outcome of each instance in accordance with TBII values and the grade of the appearance of intrathyroidal lymphocytes at the time of the operation. However, it might be possible to predict at least the recurrence of hyperthyroidism by the consideration of changes of TBII values postoperatively.

Adolescent↗
PubMed1994

An experimental transplantable osteosarcoma with spontaneous pulmonary metastasis in hamsters.

Animal models of osteosarcoma with spontaneous pulmonary metastasis which retain metastatic capacity and osteoid formation after serial passages have been reported infrequently. In this communication we describe some biological features of a transplantable osteosarcoma, Os515, induced by BK-virus in Syrian golden hamsters. The subcutaneously transplanted tumours in 2-week-old animals grew progressively until death, with a mean survival time of 32 days. Distant metastases occurred only in the lungs in all animals. The histological appearance was osteosarcoma of osteoblastic type. Enzyme-histochemical staining showed alkaline phosphatase activity in many cells and beta-glucuronidase activity in few cells. Tumours transplanted intramuscularly in the hind limbs were amputated radically at 5 or 11 days. A small number of animals died from lung metastases without local relapse during the observation period of 140 days after grafting. All the control hamsters bearing unamputated tumours died much earlier. Necropsy revealed large metastatic nodules in the lungs of limb-amputated animals and small diffuse nodules in the lungs of untreated control animals. The development of lung metastases was monitored by soft X-ray without sacrificing the animals. This model will be useful in studies of mechanisms of metastasis and for the experimental treatment of osteosarcoma.

Animals↗
PubMed1993

Development of hypothyroidism with thyroid stimulation blocking antibody long after treatment with antithyroid drugs in a patient with hyperthyroid Graves' disease: a case report.

We observed a patient manifesting spontaneous hypothyroidism with thyroid stimulation blocking antibody (TSBAb) long after treatment with antithyroid drugs (ATDs) for hyperthyroid Graves' disease. A 19-year-old female with Graves' disease was treated with ATDs for approximately 2 years; after cessation of ATDs, hyperthyroidism did not recur. Nine years later, she was again seen in our hospital because of symptoms indicative of hypothyroidism. Thyroid hormone replacement was commenced after laboratory confirmation of hypothyroidism. TSH-binding inhibitor immunoglobulin and TSBAb were both positive, while thyroid stimulating antibody (TSAb) was negative, at the time of diagnosis of hypothyroidism. These results indicated that the alterations in thyroid function in this patient appeared to relate to the presumed decline in the activity of TSAb and the appearance of TSBAb years after ATDs administration had been discontinued.

Adult↗
PubMed1993

Clinical evaluation of upper mediastinal dissection for differentiated thyroid carcinoma.

BACKGROUND: An extensive upper mediastinal dissection in advanced differentiated thyroid carcinoma is occasionally required. This investigation was undertaken to clarify the indications for mediastinal lymph node dissection and the route of upper mediastinal metastases. METHODS: Twenty-one patients with differentiated thyroid cancer, who underwent their first radical operations with mediastinal dissection through a partial midline sternotomy, were enrolled in this study. Of 21 patients, 10 (48%) were found to have mediastinal lymph node metastases. RESULTS: The tumor size in the group with metastatic disease was much bigger than that in the group without metastatic disease. Histologic type and age were similar between the two groups. The extent of cervical lymph node metastases was more significant in the group with metastatic disease; in particular, all 10 patients showed more than two metastatic nodes along the internal jugular vein of the tumor-free side. CONCLUSIONS: This study indicates that metastases to the internal jugular chain on the side contralateral to the primary tumor would be an extremely important factor for indication of extensive upper mediastinal lymph node dissection after median partial sternotomy in patients with differentiated thyroid carcinoma.

Adolescent↗
PubMed1992

Development of myelination in the human fetal and infant cerebrum: a myelin basic protein immunohistochemical study.

The early development of myelination was studied by means of myelin basic protein (MBP) and luxol fast blue (LFB) stainings of large sections of the cerebral hemispheres. Myelination first occurs in the globus pallidus, pallidothalamic fibers of the posterior internal capsule and the thalamus at 25 weeks, which may be related to the cellular maturation in the globus pallidus and thalamus. Then myelination is observed in the striatum, and precentral and postcentral gyri at 35 weeks, and the anterior internal capsule and optic radiation at 37 weeks. Immunoreactivity with MBP is observed earlier and more strongly in the early myelination period than that with LFB. Thus, MBP may play an important role in myelination and its delay. The macroscopic positivity as to MBP as well as LFB staining may be related to the development of high signal intensity observed in a T1-weighted magnetic resonance imaging, which was observed 1 to 3 months after the first microscopic appearance of myelin.

Amidines↗
PubMed1991

Case report 679. Central low-grade osteosarcoma with foci of dedifferentiation.

An infrequently encountered case of well-differentiated CLOS containing dedifferentiated tumor foci in a 28-year-old Japanese man is reported. The patient died from widespread metastases with a microscopic appearance identical to the anaplastic dedifferentiated lesions of the original tumor. This is, to our knowledge, the first report of a case of CLOS with dedifferentiation at the time of initial surgery. The literature concerning CLOS was reviewed and the diagnostic features of CLOS discussed.

Adult↗
PubMed1991

Malignant amelanotic melanoma of the esophagus.

The clinical, histological, and electron microscopic features of a case of malignant amelanotic melanoma of the esophagus are described. Amelanotic melanoma is difficult to distinguish from other malignant lesions, but in our case electron microscopy was helpful in the diagnosis.

Diagnosis, Differential↗
PubMed1990

Multiple carcinomata associated with anomalous arrangement of the biliary and pancreatic duct system. A report of two cases with a literature survey.

Two rare cases of multiple neoplasms occurring in association with anomalous arrangement of the pancreatobiliary duct system (PBD) are reported. Ultrasonography, computed tomography and endoscopic retrograde cholangiopancreatography revealed cholecystic tumor in the first case, and multiple choledochocholecystic tumors in the second case. In the first case, pancreatic tumor was discovered two years after cholecystectomy. In the second case, a pancreatic tumor was discovered incidentally in the removed pancreatic head after the operation. The histology of all of these biliary and pancreatic tumors revealed papillary adenocarcinoma in both patients, and additionally small pancreatic endocrine tumors were found in the first case. Postoperative prognosis was comfortably favorable in both patients. In a literature survey, two other cases of double carcinomata associated with anomalous PBD were found, in which the tumors showed the same histology of papillary adenocarcinoma and the postoperative prognoses were also good. These mutual clinicopathological features appear to suggest that this abnormal condition acts as a carcinogenetic risk factor in the pancreatic duct and the biliary duct system, and may finally cause multiple carcinomata in the PBD.

Adult↗
PubMed1990

[Clinical evaluation of extended neck differentiated thyroid carcinoma].

In some patients with advanced differentiated thyroid carcinoma, an upper mediastinal dissection is recommended. Both 17 primary and 6 non-primary patients who had undergone an upper mediastinal dissection in Shinshu University Hospital, from 1984 to 1987, were surveyed. We also studied 198 patients who had been performed a modified radical neck dissection from 1984 to 1986. In the 17 primary cases, 6 (35%) had positive nodes in the upper mediastinum (M group), and 11 (65%) negative (NM group). And we analysed these two groups. In addition, 5 non-primary cases and 198 patients were analyzed in terms of the nodal metastatic status of the tumor-free side. From these results, we conclude the indication of mediastinal dissection for thyroid carcinoma as follows: (1) positive lymph node is suspected in the upper mediastinum by the study of CT or 201thallium scintigram. (2) nodal metastases are suspected in the deep cervical location of the tumor-free side. (3) a male patient whose age is younger than 50, and tumor is located in the left lobe and its size is larger than 3.0 cm.

Carcinoma↗
PubMed1989

Intraductal papillary neoplasms of the pancreas. A clinicopathologic study of six patients.

A clinicopathologic study was conducted on six patients with intraductal papillary neoplasms of the pancreas. The patients were of both sexes and their ages ranged from 64 to 79 years. Three patients had a long history of symptoms mimicking chronic pancreatitis. The tumors involved the main pancreatic duct in the head-body region either diffusely or focally. Histologic examination showed papillary proliferations of well-differentiated, mucus-secreting cells that occasionally stained for carcinoembryonic antigen (CEA) and carbohydrate antigen (CA 19-9). The proliferations filled the main pancreatic duct, and extended into smaller ducts in some cases. In three patients, the lesions contained foci of pronounced to severe cellular atypia and carcinoma in situ. None of our series or any similar cases reported in the literature has shown invasion into peripancreatic tissue, metastasis, or tumor recurrence after pancreatectomy. Because of their favorable prognosis, intraductal papillary neoplasms should be considered low-grade malignancies that must not be confused with the common ductal adenocarcinoma.

Adenocarcinoma, Papillary↗
PubMed1988

Childhood lymphoma. A clinicopathological and immunohistological study of 58 cases.

Fifty-eight cases diagnosed as malignant lymphoma in patients younger than 15 years between 1976 and 1986 in the Kanto area were reviewed and reclassified as follow: 48 non-Hodgkin's lymphomas, 9 Hodgkin's disease and one malignant histiocytosis. Lymphoblastic type consisted of 26 cases or 54.2%; large cell type, 11 cases or 22.9%; Burkitt's type, 7 cases or 14.5%; medium-sized cell type and mixed cell type consisted of 4 cases. There was no follicular lymphoma case. A rare sclerosing mediastinal lymphoblastic lymphoma and diffuse large cell lymphomas with T-zone involvement as well as primary epidural Burkitt's lymphomas were found. Immunohistochemical studies using paraffin sections were performed in 43 non-Hodgkin's lymphomas and phenotypes of 37 cases were determined as follows; T cell origin in 24, B cell origin in 11 and non-T non-B in 2 cases. Of 25 lymphoblastic lymphomas, LCA was positive only in 11 cases. Reed-Sternberg cells and their variants of Hodgkin's disease reacted with anti-Leu M1 antibody in 3 of 8 examined but not with EMA antibody. This study revealed that the survival was related to sites of the primary lesion, regardless of histological type and immunologic phenotypes.

Adolescent↗
PubMed1987

Primary spinal epidural Burkitt's lymphoma.

Three Japanese children suffered from symptoms of spinal cord compression, and myelography demonstrated extradural masses compressing the spinal cord at different levels. Decompressive laminectomy revealed epidural tumors which were resected as completely as possible. Histologic pictures of the tumors showed Burkitt's lymphoma, and the diagnosis of primary epidural Burkitt's lymphoma with clinical stage A was made in each case after postoperative examination. Though postoperative radiotherapy and systemic chemotherapy were administered to all patients, they only survived for a short period of time.

Burkitt Lymphoma↗
PubMed1986

Malignant clear cell cystadenoma of the ovary.

A 50-year-old female had suffered from abdominal distension and a lower abdominal mass for 3 months. Ultrasonographic examination demonstrated a myoma uteri pattern with a large cystic ovarian tumor. Laparotomy was done on August 30, 1983 and simple total hysterectomy with bilateral salpingo-oophorectomy was carried out. FIGO stage IIC ovarian cancer was stated clinically. Histologically, clear cell cystadenoma of right ovary having benign, proliferative and malignant epithelial elements without dense ovarian stroma was found and diagnosed as malignant clear cell cystadenoma. In spite of Cisplatin therapy, the patient expired on April 6, 1984.

Ascites↗
PubMed1986

Uterine tumor resembling ovarian sex-cord tumor with osteoid metaplasia.

A rare uterine tumor showing sex-cord-like differentiation and marked osteoid metaplasia was found in a 33-year-old female. The tumor, occupying the uterine fundus, was multilobular, fleshy, and yellowish white, with punctations and poorly defined tumor margins into the subserosa. The tumor nodules were mostly composed of small, round, rather regular neoplastic cells with scanty cytoplasm and prominent nuclei. Epithelioid neoplastic cells with striking ovarian sex-cord-like arrangement, surrounded by fibrous tissue, were noticed. Polygonal neoplastic cells with marked osteoid metaplasia were also detected. The authors introduce an infrequent variant of uterine tumor resembling ovarian sex-cord tumor with osteoid metaplasia, probably of endometrial stromal cell origin. Because of deep myometrial infiltration without vascular invasion, malignant potential is considered. The patient is now alive without evidence of local recurrence or distant metastasis after abdominal total hysterectomy.

Adult↗
PubMed1985

A cystic adenomatoid tumor of the uterus simulating lymphangioma grossly.

We are reporting a rare case of an adenomatoid tumor of the uterus having multicystic gross appearance. A 32-year-old woman complaining of dysmenorrhea had multicystic mass lesions on the posterior wall of the right cornual region of the uterus. The specimen showed a honeycomb appearance with mucoid content. Microscopically, numerous gland-like spaces lined with low cuboidal cells were observed beneath the serosa, and mucopolysaccharide material accumulated in the cystic spaces forming, honeycomb-like lesions surrounded by myometrial tissue. The postoperative course was uneventful and the patient was discharged on the 9th day after the operation. "Cystic adenomatoid tumor of the uterus" was diagnosed. The patient is being followed up continuously, so far without recurrence.

Adenoma↗