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Biomedical subjects

K Arashidani

Publications and source records attributed to K Arashidani.

41 records · Page 3Linked to original sources
PubMed1985

[A simple determination of phenol in urine by high performance liquid chromatography].

A simple analytical method for urinary phenol by high performance liquid chromatography was tested. This procedure consists of the following three steps: hydrolysis of urine with hydrochloric acid, extraction of phenols with isopropylether, separation and determination of phenol by high performance liquid chromatography, under the following operating conditions: column, LiChrosorb RP 18 (250 mm X 4 mm, I.D.); mobile phase, acetonitrile-water (3: 7, v/v) with a flow rate of 0.8 ml/min; column temperature, 40 degrees C; detection, UV monitor at 270 nm. The detection limit (signal-to-noise ratio = 2) was 1 ng. The analytical results by this method were compared with those obtained by another method used for gas chromatography. The analytical results obtained by the two methods were in good agreement (r = 0.998) for 30 normal urine samples tested. The geometrical average value for phenol in normal urine samples (n = 30) was 8.2 micrograms/ml. Therefore this method was proved to be useful for biological monitoring of benzene exposure.

Adult↗
PubMed1983

[Air pollution survey in Kitakyushu district].

Airborne particulate samples were collected on a glass fiber filter or quartz filter using Hi-volume air sampler from November, 1980 through February, 1983 at a west part of Yahata district, Japan. The concentrations of airborne particulates, polynuclear aromatic hydrocarbons (PAH), heavy metals and dustfall were determined, and mutagenic activities of tarry materials obtained from suspended particulates were also measured. The following results were obtained. (1) The airborne particulate contents were 22.1 - 188 mg/1000 m3 (mean : 74.6 mg/1000 m3), and the values were high in spring and low in summer. (2) PAH contents in airborne particulates were in the following order: benzo(ghi)perylene greater than benzo(a)pyrene greater than benz(a)anthracene greater than chrysene greater than benzo(k)fluoranthene greater than pyrene greater than perylene. PAH contents were higher in winter that in summer. The benzo(a)pyrene contents were 1.97 micrograms/1000 m3 in 1981 and 1.92 micrograms/1000 m3 in 1982. (3) Heavy metals content was about 2.1 - 3.6 higher in winter in comparison with that in summer time. (4) Mutagenic activity showed 90 - 11900 revertants/1000 m3 for TA 98 strain with S-9 mix and 50 - 7190 revertants/1000 m3 for TA 98 strain without S-9 mix. Mutagenic activities for TA 98 with S-9 mix were higher than those for TA 98 without S-9 mix. (5) As a result of the analysis of airborne particulate samples, a significant correlation was observed between mutagenic activities and the concentrations of PAH and heavy metals. These results indicated that the mutagenic survey may be useful as an index for air pollution study.

Air Pollutants↗
PubMed1982

Endothelial specific granules in the umbilical veins of the postnatal rabbit.

A remarkable increase in number of endothelial specific granules was observed in the rabbit umbilical veins between 2 and 5 days after birth. Electron microscopy indicated that the granules were segregated in the Golgi complex of the endothelial cells and released into the vascular lumen during the postnatal obliteration stage of this vessel. Incubation of the postnatal vessels in Ringer solution containing a histamine-releasing compound induced remarkable morphological alterations of these cytoplasmic components; a reduction of their osmiophilia, swelling with a widened space separating the granular matrix from the limiting membrane, fusion to each other and expulsion of their contents into the vascular lumen, as in mast cell degranulation by this drug, were noted. High-performance liquid chromatography of the homogenized vessels demonstrated appreciable concentrations of histamine in the postnatal samples. There was a correlation between the histamine concentration and the quantity of granules in the respective postnatal samples. The present study strongly suggests that the granules are reservoirs of histamine and have an important role in the obliteration of this vessel.

Animals↗