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K Apel

Publications and source records attributed to K Apel.

78 records · Page 5Linked to original sources
PubMed1976

Light-induced increase in the activity of maize plastid DNA-dependent RNA polymerase.

Illumination of dark-grown maize plants induces a rapid rise in DNA-DEPENDENT RNA polymerase activity in plastids. Within 16 h illumination the enzyme activity is isolated plastids increases 3-4 fold as compared to other dark-grown plants illuminated for only 2 h. This change in activity is unaccompanied by either a quantitative rise in the amount of chloroplast RNA polymerase or qualitative changes in the purified enzyme. It is suggested that other factors may be present which interact either with the enzyme or the DNA template and that these factors are responsible for the light-induced enhancement of the RNA polymerase activity inplastids.

Chlorophyll↗
PubMed1976

Chloroplast membranes of the green alga Acetabularia mediterranea. II. Topography of the chloroplast membrane.

The localization of the chlorophyll-protein complexes inside the thylakoid membrane of Acetabularia mediterranea was determined by fractionating the chloroplast membrane with EDTA and Triton X-100, by using pronase treatment, and by labeling the surface-exposed proteins with 125I. The effects of the various treatments were established by electrophoresis of the solubilized membrane fractions and electron microscopy. After EDTA and pronase treatment, the membrane structure was still intact. Only the two chlorophyll-protein complexes of 67,000 and 152,000 daltons and an additional polypeptides were found in the membrane before the EDTA and pronase treatment. The 125,000 dalton complex seems to be buried inside the lipid layer. The 23,000 dalton subunit of the 67,000 dalton complex is largely exposed to the surface of the EDTA-insoluble membrane and only the chlorophyll-binding subunit of 21,500 daltons is buried inside the lipid layer.

Acetabularia↗
PubMed1975

Chloroplast membranes of the green alga Acetabularia mediterranea. I. Isolation of the photosystem II.

1. In the presence of Triton X-100, chloroplast membranes of the green alga Acetabularia mediterranea were disrupted into two subchloroplast fragments which differed in buoyant density. Each of these fractions had distinct and unique complements of polypeptides, indicating an almost complete separation of the two fragments. 2. One of the two subchloroplast fractions was enriched in chlorophyll b. It exhibited Photosystem II activity, was highly fluorescent and was composed of particles of approx. 50 A diameter. 3. The light-harvesting chlorophyll-protein complex of the Photosystem II-active fraction had a molecular weight of 67 000 and contained two different subunits of 23 000 and 21 500. The molecular ratio of these two subunits was 2:1.

Acetabularia↗