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Biomedical subjects

K Anzai

Publications and source records attributed to K Anzai.

118 records · Page 7Linked to original sources
PubMed1977

Thermotropic transition in the states of proteins in sarcoplasmic reticulum vesicles.

Interaction between protein and phospholipid molecules in sarcoplasmic reticulum (SR) was studied by measurement of the conformational fluctuation of the protein using the kinetics of the hydrogen-deuterium exchange reaction. It was revealed that the state of SR ATPase undergoes a phase transition at about 18 degrees C with boundary lipids, corresponding to a discrete change in the activation energy of the Ca2+, Mg2+-ATPase reaction.

Animals↗
PubMedDate not supplied

Change with donor age in the degradation rate of endogenous proteins of mouse hepatocytes in primary culture.

The degradation rate of [(3)H]leucine labeled intracellular proteins was compared in hepatocytes derived from young (4-7-month-old) and aged (23-month-old) mice. Average half-lives of long-lived proteins were 40 h and 65 h in the cells from young and old animals, respectively. This observation confirms our earlier results that half-lives of foreign proteins introduced into the cells from old mice are 50-60% longer than those of younger counterparts (Ishigami and Goto, 1988, Mech. Ageing Dev., 46, 125-133; Ishigami and Goto, 1990, Arch. Biochem. Biophys., 277, 189-195).

Journal Article↗
PubMedDate not supplied

Reversal of nonresponders and postexposure prophylaxis by intradermal hepatitis B vaccination in Japanese medical personnel.

Thirty-one Japanese nonresponders to subcutaneous hepatitis B vaccination and 15 medical personnel who were accidentally exposed to specimens positive for hepatitis B e antigen and were given hepatitis B immunoglobulin were intradermally immunized with 5 micrograms of plasma-derived hepatitis B vaccine every 2 weeks until delayed type hypersensitivity skin reaction to hepatitis B surface antigen became positive. Thirty (97%) of the 31 nonresponders developed delayed type hypersensitivity skin reactions to hepatitis B surface antigen after 2.3 +/- 1.2 (mean +/- SD) revaccinations. Twenty-nine (94%) of the 31 nonresponders had anti-hepatitis B surface antigen antibody levels greater than 10 IU/L. The immunoglobulin subclass of the antibody to hepatitis B surface antigen was mainly IgG1. After 1 year, 23 (74%) of the 31 nonresponders continued to have anti-hepatitis B surface antigen antibody levels greater than 10 IU/L. Persons accidentally exposed to specimens positive for hepatitis B e antigen developed delayed type hypersensitivity skin reactions to hepatitis B surface antigen following 3.1 +/- 1.1 revaccinations. None developed clinical hepatitis. There was no production of anti-hepatitis B core antigen antibody 1 year after exposure, indicating that protection was 100%. Intradermal hepatitis B vaccination is useful in reversing nonresponsiveness to hepatitis B surface antigen and for prophylaxis after exposure.

Adult↗