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Biomedical subjects

K Alonso

Publications and source records attributed to K Alonso.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Effects of environmentally relevant concentrations of 2,3,7,8-TCDD on domestic chicken immune function and CYP450 activity: F1 generation and egg injection studies.

Domestic chickens (Gallus gallus) were used as a surrogate species for wild turkey to assess risk from environmental 2,3,7,8-TCDD exposure. Lymphocyte proliferation and CYP450 induction were assessed in adults exposed via i.m. injection, in F1 14-day old hatchlings, in F1 adults (30-weeks old), and in 14-day old hatchlings exposed via yolk sac injections. Hatchlings from injected eggs exhibited a dose-response in lymphocyte proliferation, IgM titers, EROD, and PROD endpoints. Exposed adults showed a significant dose-dependent increase in CYP450 induction. F1 14-day old chicks exhibited a significant dose-dependent suppression of B-cell proliferation and induction of CYP450 enzymes. F1 adult proliferative responses exhibited B-cell suppression, that was not statistically significant. Significant sex-dependent EROD and MROD induction was also observed in F1 adults, indicating mixed-function oxidase imprinting from maternal exposure.

Animals↗
PubMedDate not supplied

Modification of functional serum antithrombin III assay for fibrometer. Results of examination of 50 patients.

The functional antithrombin III assay of von Kaulla is described and modified for the fibrometer with substitution of readily available Owren's buffer for previously described buffers. The assay is compared with immunological techniques on 50 hospital patients and comparable discrimination of normals and patients with thrombotic disease are shown. Normal values are 51.1+/-13.9 secs. Results in patients with thrombotic diseases are 16.7+/-1.8 secs.

Antithrombins↗
PubMedDate not supplied

Estrogen receptors by immunofluorescence: comparison with a dextran coated charcoal technique.

Biopsy specimens from 21 women with primary operable breast cancer were analyzed in a double blind study designed to compare fluorescent antibody methods for detection of estrogen receptors with estrogen receptors measured biochemically with a previously reported dextran coated charcoal technique (DCC). Fluorescent antibody methoids employed both direct techniques: (1) rabbit antisera to 6-O-Carboxymethyl-Oxime-17 beta Estradiol tagged with Fluorescin Istthiocyanate (FITC) and (2) indirect techniques (sheep antisera to 11-Hemisuccinate-17 beta Estradiol reacted wih FITC tagged rabbit antisheep sera). Fluorescent antibody methods showed 100 percent correlation with each other but only 67 percent correlation with the DCC assay. Fluorescence was striking when present. Molecular forms were not easily predicted. Divergent results occurred largely in specimes sparsely populated with malignant cells (18 percent) though neoplasms showing no fluorescence demonstrated elevated receptor conconcentrations with DCC (15 percent). Indirect fluorescent techniques are the simplest of those studied. Fluorescent techniques have not replaced biochemical techniques in the authors' laboratories.

Breast Neoplasms↗