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Biomedical subjects

K Aihara

Publications and source records attributed to K Aihara.

At least 127 records · Page 7Linked to original sources
PubMed1979

Early changes in the arterial endothelium under various pathological conditions: an electron histochemical study with visualization of altered permeability using electron microscopic tracers.

The authors have studied the endothelial changes in the aorta and the arteries of heart, kidney and other organs in cholesterol fed rabbits and endotoxin administrated rats through electron histochemical and vascular clearance methods. The vascular changes are variable presumably due to the functional and morphological difference of vessels. The administration of cholesterol and endotoxin may result in increased vascular permeability which is directly related to the qualitative increase and decrease of hydrolytic enzymes in the vascular wall. The permeability of high molecular substances was enhanced with the widening of the intercellular space in contraction of endothelium and the changing of molecular structure of plasma membrane accompanied with plasma lipid, platelets and hemodynamic stress. This fact was confirmed by use of horseradish peroxidase and dextran fesin as tracers of different molecular sizes. The authors emphasize that direct injury and subsequent abnormal contraction of the endothelial cell may initiate increased permeability of plasma protein and lipid in the medial layer and eventually may develop and enhance arteriosclerosis.

Acid Phosphatase↗
PubMed1978

A xerographic method for the quantitative assessment of atherosclerotic lesions.

A new method has been developed for the quantitative assessment of atherosclerotic lesions, in which a conventional xerox machine is the sole equipment used. As an example, the aorta from a cholesterol-fed rabbit was removed in toto, opened longitudinally along its anterior margin, flattened in a transparent polypropylene bag and then xeroxed. The outlines of the atherosclerotic lesions were traced on translucent graph paper ruled into 1 mm squares. The squares within those outlines were counted and expressed as a percentage of the whole surface. This xerographic method is rapid and reproducible and is achieved without changing the magnification; it gives comparable or better results than other staining methods. As the aortic specimen is undamaged by fixation or staining, the specimen can be used for subsequent morphological or biochemical studies. Thus, the degree of surface involvement can be correlated with other morphological or biochemical changes in the same arterial samples.

Animals↗
PubMed1977

The role of cathepsin B1 in the collagen breakdown of carrageenin granuloma in rats.

The crude cathepsin B1 isolated from granulation tissue was separated into two major peaks (P-2 and P-3 fractions) by chromatography on CM-cellulose. The changes in cathepsin B1 activity were studied at various phases of the development of granulation tissue. The cathepsin B1 activity of P-2 fraction, in contrast with that of P-3 fraction, increased with the passage of time after carrageenin injection and the cathepsin B1 activity of P-2 fraction was higher in the resorbing tissues than in non-resorbing tissues, suggesting that cathepsin B1 in P-2 fraction plays an important role in the resorption of granulation tissue, including collagen breakdown.

Animals↗