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K Agematsu

Publications and source records attributed to K Agematsu.

59 records · Page 4Linked to original sources
PubMed1991

Recipient origin of bone marrow-derived fibroblastic stromal cells during all periods following bone marrow transplantation in humans.

The bone marrow microenvironment, which is composed of fibroblasts, endothelial cells, adipocytes and macrophages, plays an important role in the haematopoiesis and lymphopoiesis by producing various cytokines. Therefore, investigation of the origin of these cells following allogeneic bone marrow transplantation (BMT) is very significant, in terms of the haematological and immunological reconstitution after BMT. We have investigated the origin of fibroblastic stromal cells in long-term cultures in seven of the sex-mismatched cases. This was carried out by in situ hybridization using a Y-chromosome specific cDNA probe (PHY10), conserving the morphology of the cells. In situ hybridization analysis showed that bone marrow fibroblasts (BMF) in long-term cultures in all the sex-mismatched cases originated from the recipients. We have also performed Southern blot analysis using a PHY10 probe in the sex-mismatched cases and using a variable number of tandem repeats (VNTR) probe, which can detect DNA polymorphisms, in two fo the sex-matched cases. In addition, we have employed polymerase chain reaction (PCR) using the VNTR marker (MCT118). Although all the patients showed haemopoietic engraftment with donor cells, their BMF were found, by Southern blot analysis and PCR method, to be of the recipient origin. These data indicate that bone marrow-derived fibroblastic stromal cells which proliferate in long-term cultures are not transplantable in the conditioning regimens used for allogeneic BMT in humans.

Adolescent↗
PubMed1991

[Bone marrow transplantation in a pediatric case of acute nonlymphocytic leukemia with hepatitis C].

An allogenic bone marrow transplantation (BMT) in an acute nonlymphocytic leukemia (ANLL) patient with post-transfusion hepatitis C is presented. A 13-year-old girl was admitted to our hospital on May 1988, and diagnosed as having ANLL M2 according to the FAB classification. During the induction and post-induction chemotherapy, 116 units of blood products were transfused to her as the supportive therapy until October 1988, when non-A non-B hepatitis developed. As the persistent liver dysfunction interfered with anti-leukemic chemotherapy on the protocol, allogeneic BMT from her HLA identical MLR nonreactive brother was done on July 1989. Preconditioning regimen consisted of busulfan and cyclophosphamide. GVHD prophylaxis consisted of cyclosporine A and short term methotrexate. After the BMT, her liver dysfunction once improved; her serum amino-transferase levels were normal for about 3 months. Soon after discontinuation of cyclosporine A, however, her liver function deteriorated again. The examination of hepatitis C virus antibody in her sera, which had been harvested sequentially and stored at -40 degrees C, on November 1989 revealed that she had been already seropositive at the time of BMT. The BMT-induced immunologic changes may have influenced the natural course of hepatitis C virus infection in the patient.

Adolescent↗
PubMed1990

Detection of engraftment and chimerism after bone marrow transplantation by in situ hybridization using a Y-chromosome specific probe.

After bone marrow transplantation (BMT), the recipient and donor cells must be distinguished from each other to document and characterize successful engraftment. In addition to dot blot and Southern blot analyses, we have performed in situ hybridization in two sex-mismatched cases using a Y-chromosome specific DNA probe (PHY10). In situ hybridization showed that greater than 95% of the peripheral mononuclear cells had clusters of grains indicative of male cell origin in a recipient girl (case 1), and no cells had clusters of grains in another recipient boy (case 2) at the time of engraftment and 3 months after BMT. In situ hybridization using the PHY10 probe appears to facilitate identification of individual cells of male and female origin, and it requires only 20 hr to obtain the results. The technique provides a powerful new method for the documentation of engraftment and the detection of mixed hematopoietic chimerism in peripheral blood and bone marrow cell compartments after BMT.

Blotting, Southern↗
PubMed1988

Chondrodysplasia punctata with X;Y translocation.

We have studied a family in which the mother and her son were carriers of an X;Y translocation, der(X)t(X;Y) (p22.3;q11). The mother was of slightly short stature and had mildly short upper extremities. The son had epiphyseal punctate calcifications, mildly short extremities, a flattened nasal bridge, and mental retardation (chondrodysplasia punctata). The extra bands on the short arm of the X chromosome were identified as deriving from the long arm of the Y chromosome, using in situ hybridization with a Y-chromosome-specific DNA probe (pHY10). The chondrodysplasia punctata seen in our case may be associated with the abnormality of the distal short arm of the X chromosome caused by X;Y translocation.

Chondrodysplasia Punctata↗