Biomedical subjects

K A Dorph-Petersen

Publications and source records attributed to K A Dorph-Petersen.

3 recordsLinked to original sources

PubMed2001

Tissue shrinkage and unbiased stereological estimation of particle number and size.

This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paper introduces new estimators to be used in optical fractionator and optical disector designs; the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.

Brain↗

PubMed2000

Non-uniform systematic sampling in stereology.

Non-uniform systematic sampling designs in stereology are studied. Various methods of constructing non-uniform systematic sampling points from prior knowledge of the measurement function are presented. As an example, we consider area estimation from lengths of linear intercepts. The efficiency of two area estimators, based on non-uniform sampling of parallel lines, is compared to that of the classical 2D Cavalieri estimator, based on uniform sampling, in a sample of planar profiles from transverse sections of 41 small myelinated axons. The comparison is based on simulations. It is concluded that for profiles of this type one of the non-uniform sampling schemes is more efficient than the traditional uniform sampling scheme. Other examples where non-uniform systematic sampling may be used are in area estimation from lines emanating from a fixed point, area estimation from concentric circles or spirals and curve length estimation from sweeping lines. It is shown that proportional-to-size sampling is a special case of non-uniform systematic sampling. Finally, the effect of noise in the observations is discussed.

Axons↗

PubMed1999

Stereological estimation using vertical sections in a complex tissue.

A method designed for stereological estimation in a very complex tissue using vertical sections is presented. In some tissues, the random rotation of the tissue for vertical sections may obscure recognition of the anatomical structures of interest. The present method overcomes this problem by generating sections with both a particular orientation, 'mapping sections', and ordinary random vertical sections usable for the required observations. A map describing the positions of the vertical sections is produced to make the complex reference space recognizable. The method is illustrated by estimating the number and size of neurones in the dorsal raphe nucleus of the human brainstem with its dense packing of roughly 100 nuclei within a volume less than 50 cm3.

Anatomy, Cross-Sectional↗