Biomedical subjects

Jungchul Kim

Publications and source records attributed to Jungchul Kim.

4 recordsLinked to original sources

PubMed2006

Survival rate according to grafted density of Korean one-hair follicular units with a hair transplant implanter: Experience with four patients.

BACKGROUND: The survival rate of grafted hairs in hair transplantation has been frequently discussed by many hair-transplant surgeons. Several factors, including skill of doctors and assistants and the amount of perifollicular connective tissue, are suggested to have predictive values in foretelling the eventual quantity of hair growth. OBJECTIVE: The purpose of this study is to determine survival rate according to density of Korean one-hair follicular units grafted with a KNU implanter. METHODS: We designed four templates of 1 cm2 made by tattooing two templates on each side of empty frontoparietal recesses in four Korean patients with male pattern alopecia (beyond Norwood-Hamilton-Type IIIa). One-hair follicular units were prepared from occiput and we grafted 20, 30, 40, and 50 one-hair follicular units using a KNU implanter on each template, respectively, and counted the number of surviving hairs after 10 months. RESULTS: The survival rate of 20 and 30 grafts per template was higher than that of 40 and 50 grafts. CONCLUSION: We think that 30 one-hair follicular units grafted with a KNU implanter is the proper density for better survival in Korean hair transplantation, and repeated sessions will be necessary for a better final result. However, we need further evaluation with a larger number of participants to overcome the small sample size and limitation of one-hair follicular unit grafts. The authors have indicated no significant interest with commercial supporters.

Adult↗

PubMed2005

Gene expression profiles in varicose veins using complementary DNA microarray.

BACKGROUND: There has been little information reported about the genetic event concerning the pathophysiology of varicose vein (VV). OBJECTIVES: The purpose of this study was to examine the differentiation of gene expression in the wall of VV using complementary deoxyribonucleic acid (cDNA) microarrays. METHODS: The study was performed with four pairs of VVs and control veins (CVs). cDNA specimens of VVs were prepared from the ribonucleic acid-isolated VVs of patients who underwent venous obliteration, using radiofrequency, as well as from CVs of those who underwent aortocoronary bypass grafting. Each set of VVs and CVs was hybridized with high-density microarray containing 3,063 human cDNAs. The finding of microarray hybridization were scanned, analyzed, and classified with the cluster program. RESULTS: Among 3,063 cDNA clones, 82 genes were up-regulated in VVs, and some of the up-regulated genes, which were detected by cDNA microarray, including transforming growth factor 3-induced gene (BIGH3), tubulin, lumican, actinin, collagen type I, versican, actin, and tropomyosin, belonged to extracelluar matrix molecules, cytoskeletal proteins, or myofibroblasts. CONCLUSIONS: Many up-regulated genes were found in Ws by applying cDNA microarray. These gene profiles suggested a pathway associated with fibrosis and that wound healing might be related to the pathophysiology of VVs.

DNA, Complementary↗

PubMed2003

Detection of human papillomavirus 60 in epidermal cysts of nonpalmoplantar location.

Human papillomavirus (HPV) types 57 and 60 are associated with epidermal cysts of palmoplantar location (PPECs). Recently, there was a report of HPV 60 in a wart located in a nonpalmoplantar area as a possible precursor of a nonpalmoplantar epidermal cyst (NPPEC). There has been no study that has examined the presence and frequency of HPV in ordinary NPPECs. We reviewed 63 cases of epidermal cysts, including 59 NPPECs and 4 PPECs. After routine histopathologic review, polymerase chain reaction (PCR) of extracted DNA, sequencing of PCR products, and, finally, a homology search were undertaken. As a result, 9 of 63 (14.3%) cysts, including 6 of 59 NPPECs (10.2%), showed a positive reaction on PCR. By homology search after PCR, all were demonstrated as HPV 60. For NPPECs, no variables, including some histopathologic features, are significantly related to the presence of HPV 60 in univariate or multivariate analysis (P > 0.05).

Adolescent↗

PubMedDate not supplied

Discovery of differentially expressed genes related to histological subtype of hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is one of the most common human malignancies in the world. To identify the histological subtype-specific genes of HCC, we analyzed the gene expression profile of 10 HCC patients by means of cDNA microarray. We proposed a systematic approach for determining the discriminatory genes and revealing the biological phenomena of HCC with cDNA microarray data. First, normalization of cDNA microarray data was performed to reduce or minimize systematic variations. On the basis of the suitably normalized data, we identified specific genes involved in histological subtype of HCC. Two classification methods, Fisher's discriminant analysis (FDA) and support vector machine (SVM), were used to evaluate the reliability of the selected genes and discriminate the histological subtypes of HCC. This study may provide a clue for the needs of different chemotherapy and the reason for heterogeneity of the clinical responses according to histological subtypes.

Algorithms↗