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Biomedical subjects

Jonathan Leach

Publications and source records attributed to Jonathan Leach.

7 recordsLinked to original sources

An optically driven pump for microfluidics.

We demonstrate a method for generating flow within a microfluidic channel using an optically driven pump. The pump consists of two counter rotating birefringent vaterite particles trapped within a microfluidic channel and driven using optical tweezers. The transfer of spin angular momentum from a circularly polarised laser beam rotates the particles at up to 10 Hz. We show that the pump is able to displace fluid in microchannels, with flow rates of up to 200 microm(3) s(-1) (200 fL s(-1)). The direction of fluid pumping can be reversed by altering the sense of the rotation of the vaterite beads. We also incorporate a novel optical sensing method, based upon an additional probe particle, trapped within separate optical tweezers, enabling us to map the magnitude and direction of fluid flow within the channel. The techniques described in the paper have potential to be extended to drive an integrated lab-on-chip device, where pumping, flow measurement and optical sensing could all be achieved by structuring a single laser beam.

Microfluidic Analytical Techniques↗

Interactive approach to optical tweezers control.

We have developed software with an interactive user interface that can be used to generate phase holograms for use with spatial light modulators. The program utilizes different hologram design techniques, allowing the user to select an appropriate algorithm. The program can be used to generate multiple beams and can be used for beam steering. We see a major application of the program to be in optical tweezers to control the position, number, and type of optical traps.

Algorithms↗

Creating permanent 3D arrangements of isolated cells using holographic optical tweezers.

We report the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.

Cell Separation↗

Laser beams: knotted threads of darkness.

Destructive interference may lead to complete cancellation when light waves travelling in different directions cross, and in three-dimensional space this occurs along lines that are vortices of electromagnetic energy flow. Here we confirm theoretical predictions by experimentally creating combinations of optical laser beams in which these dark threads form stable loops that are linked and knotted.

Journal Article↗

Interferometric methods to measure orbital and spin, or the total angular momentum of a single photon.

We propose interferometric methods capable of measuring either the total angular momentum, or simultaneously measuring the spin and orbital angular momentum of single photons. This development enables the measurement of any angular momentum eigenstate of a single photon. The work allows the investigation of single-photon two-qubit entangled states and has implications for high density information transfer.

Journal Article↗

Measuring the orbital angular momentum of a single photon.

We propose an interferometric method for measuring the orbital angular momentum of single photons. We demonstrate its viability by sorting four different orbital angular momentum states, and are thus able to encode two bits of information on a single photon. This new approach has implications for entanglement experiments, quantum cryptography and high density information transfer.

Journal Article↗