Microarray analysis using RNA arbitrarily primed PCR.
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Cell Line↗
Biomedical subjects
John Welsh
Publications and source records attributed to John Welsh.
Bile acids mimic oxidative stress induced upregulation of thioredoxin reductase in colon cancer cell lines.
Bile acids have been suggested to play an important role in the etiology of colon and gastric cancer after gastrectomy, but the molecular biology of these effects is poorly understood. We evaluated the effect of different bile acids on human gastric and colon carcinoma cells and identified genes by RNA arbitrarily primed PCR for differential display that are modulated following treatment with hydrophobic bile acids. Thioredoxin reductase (TR) mRNA was upregulated after treatment with taurochenodeoxycholic acid (TCDCA) in St 23132 cells. This raised the question whether deoxycholic acid (DCA) would have regulative effects on TR in HT-29 cells. After an incubation time of 6 h with DCA, TR mRNA expression was increased up to threefold. Ursodeoxycholic acid had no influence on TR mRNA expression. The upregulation of TR after DCA incubation was almost identical to incubation with 12-O-tetradecanoylphorbol-13-acetate. This implies that hydrophobic bile acids mediate oxidative stress in gastrointestinal cancer cells, which was confirmed by measurement of oxidative burst after treatment with DCA. The results suggest that hydrophobic bile acids induce oxidative stress in gastrointestinal cancer resulting in a compensatory upregulation of TR mRNA, one of the key components in the complex anti-oxidant defense system within eukaryotic cells. The activation of at least parts of the redox signaling system is potentially related to the cytotoxicity and the stimulation of the cell death machinery induced by toxic bile acids.
Base Sequence↗
The experience of parents of children with primary lymphoedema.
This article presents the findings of a qualitative study looking at the experiences of parents of children with primary lymphoedema. Six parents participated in the study, which was conducted in Glasgow, and verbatim transcripts of the interviews were analysed for recurring themes. The findings showed that these parents endured a difficult time before receiving a diagnosis and appropriate treatment, caused by a lack of professional awareness of the condition. Their day-to-day management of the illness was hampered by the psychological effects on the children. Parents also found a reluctance by professionals to seek information on their behalf. Professional awareness of this condition needs to be raised and improved information and support made available for those involved in the care of children with lymphoedema. The article discusses how this may be achieved.
Adaptation, Psychological↗