Biomedical subjects

Joanna Puławska

Publications and source records attributed to Joanna Puławska.

4 recordsLinked to original sources

PubMed2005

Rapid and specific identification of four Agrobacterium species and biovars using multiplex PCR.

On the basis of 23S rRNA gene sequences, 1 universal forward and 4 taxon (species/biovar)-specific reverse primers were designed for multiplex PCR to aid in identification and differentiation of Agrobacterium rubi, Agrobacterium vitis and Agrobacterium biovars 1 and 2. In reactions with DNA of 119 bacterial strains belonging to: Agrobacterium, Allorhizobium, Mesorhizobium, Rhizobium, Sinorhizobium and Phyllobacterium, as well as phytopathogenic bacteria representing various genera, the primers developed for identification of A. vitis, A. rubi or Agrobacterium biovar 1 amplified only DNA of strains belonging to these taxa, producing fragments of the expected sizes: 478, 1006 and 184bp, respectively. However, in the case of the primer developed for identification of Agrobacterium biovar 2, the characteristic 1066bp PCR product was obtained not only with DNA of this biovar, but also with DNA of 3 atypical biovar 1 strains and some rhizobial strains. Differentiation between Agrobacterium biovar 2 and the other strains was possible using the restriction analysis of this product with endonuclease Alw26I. The method developed is an excellent tool for rapid classification of these 4 taxa of Agrobacterium.

Bacterial Typing Techniques↗

PubMed2004

Distant consequences of traumatic injuries to deciduous teeth--a case report.

The ever increasing number of injuries to deciduous teeth in children constitutes a serious problem in paedodontics. Among many factors that facilitate tooth injuries are malocclusions, and most specifically protrusions of upper incisors of Class II/1 according to Angle. This is a case report of a 6-year-old girl with irregularities of upper permanent incisors due to traumatic injuries to deciduous teeth. In view of the ongoing child development processes, medical check-ups following injuries to deciduous teeth should be undertaken once every two or three months. Since such injuries have an impact on the development of the masticatory organ, children should undergo medical inspection and observation immediately upon the completion of relevant post-trauma treatment.

Child↗

PubMed2004

The methods of treating deciduous dentition after mechanical traumas.

For the sake of very young patients and the processes of growth and development of the masticatory system they undergo, mechanical traumas of deciduous teeth require immediate treatment. The choice of treatment method of the deciduous dentition traumas depends on a type of sustained injury, the developmental stage of deciduous teeth and first of all on a child's ability to cooperate. The primary objective of the therapeutic management is to retain the physiological function of an injured tooth and to protect the regular development of a permanent tooth bud. Considering the impact of a suffered injury on the developing stomatognathic system, children should be given medical check-ups and be under the observation, even after the completed treatment of deciduous tooth post-traumatic state. It is also necessary to raise among parents pro-healthcare awareness of the necessity to report a child to immediate medical treatment. This would reduce the number of subsequent complications occurring in the secondary dentition after the injuries suffered in childhood.

Child↗

PubMed2003

Primary teeth trauma.

The goal of this study was to analyze the impact of different kinds of injuries, time elapsed before treatment and child's age on the choice of management method. 59 children with the face trauma aged from six months to five years and a half were treated between 1999 and 2002 in the Department of Paedodontics of Lublin. 76 primary teeth were injured. The most common kind of trauma was lateral luxation. The choice of treatment approach depended of age, time elapsed before trauma management and a kind of injury. The conclusions after this study analyze were: 1. The trauma management method choice of primary dentition depends on a child's age, type of trauma and time elapsed before treatment. 2. The most common kind of trauma was lateral luxation. 3. Late management after injury does not always allows for the biological treatment methods.

Child, Preschool↗