Biomedical subjects

Jingbo Cai

Publications and source records attributed to Jingbo Cai.

6 recordsLinked to original sources

PubMed2005

Stratified waveguide grating coupler for normal fiber incidence.

We propose a new stratified waveguide grating coupler (SWGC) to couple light from a fiber at normal incidence into a planar waveguide. SWGCs are designed to operate in the strong coupling regime without intermediate optics between the fiber and the waveguide. Two-dimensional finite-difference time-domain simulation in conjunction with microgenetic algorithm optimization shows that approximately 72% coupling efficiency is possible for fiber (core size of 8.3 microm and delta=0.36%) to slab waveguide (1.2-microm core and delta=3.1%) coupling. We show that the phase-matching and Bragg conditions are simultaneously satisfied through the fundamental leaky mode.

Journal Article↗

PubMed2004

Three-dimensional analysis of a hybrid photonic crystal-conventional waveguide 90 degree bend.

We present a three-dimensional (3D) analysis of a hybrid photonic crystal-conventional waveguide 90 degree bend proposed previously [Opt. Express 10, 1334 (2002)] as an ultracompact component for large-scale planar lightwave circuit integration. Both rigorous 3D finite-difference time-domain modeling and a simple perfect mirror model analysis were carried out for different Si post heights in the photonic crystal region. Results show that the bend efficiency increases rapidly with Si post height. For a post height of 6.5 microm, this structure yields a bend efficiency of 97.3% at a wavelength of 1.55 microm for 90 degree bends in 2 microm x 2 microm square channel conventional waveguides with a refractive index contrast of 3.55%, which is very close to the bend efficiency of 98.2% for the corresponding two-dimensional problem. Our 3D analysis permits the examination of issues such as out-of-plane scattering loss and the effects of finite Si post height that are not considered in two dimensions.

Journal Article↗

PubMed2003

Parallel microgenetic algorithm design for photonic crystal and waveguide structures.

We have developed a powerful parallel genetic algorithm design tool for photonic crystal and waveguide structures. The tool employs a small-population-size genetic algorithm (microgenetic algorithm) for global optimization and a two-dimensional finite-difference time-domain method to rigorously design and optimize the performance of photonic devices. We discuss the implementation and performance of this design tool. We demonstrate its application to two photonic devices, a defect taper coupler to connect conventional waveguides and photonic crystal waveguides, and a sharp 90 degrees waveguide bend for low index contrast waveguides.

Journal Article↗

PubMed2003

Ultracompact high-efficiency polarizing beam splitter with a hybrid photonic crystal and conventional waveguide structure.

We propose an ultracompact high-efficiency polarizing beam splitter that operates over a wide wavelength range and is based on a hybrid photonic crystal and a conventional waveguide structure. Within a small area (15 microm x 10 microm), this polarizing beam splitter separates TM- and TE-polarized modes into orthogonal output waveguides. Results of simulations with the two-dimensional finite-difference time-domain method show that 99.3% of TM-polarized light is deflected by the photonic crystal structure (with a 28.0-dB extinction ratio), whereas 99.0% of TE-polarized light propagates through the structure (with a 32.2-dB extinction ratio). Wave vector diagrams are employed to explain the operation of a polarizing beam splitter. Tolerance analysis reveals a large tolerance to fabrication errors.

Journal Article↗

PubMed2002

Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes.

1. In the present experiments, we investigated the effects of methylecgonidine (MEG) on nitric oxide (NO) production in cultured neonatal rat cardiomyocytes. Incubation of cultured cardiomyocytes with carbachol or MEG for 48 h significantly enhanced NO production. No release was increased from 1.48+/-0.13 microM (mg protein)(-1) for control to 5.73+/-0.19 microM (mg protein)(-1) for 1 microM carbachol treated cells (P<0.001). In addition, incubation with 1 microM MEG enhanced NO production to 5.55+/-0.28 microM (mg protein)(-1). The effects of MEG on NO production were concentration-dependent. The muscarinic antagonist atropine prevented the enhancement of NO production induced by carbachol or MEG. Compared to MEG-induced NO production, cocaine was much less potent. 2. The enhancement of NO production by carbachol or MEG was even greater in cultured cardiomyocytes transfected with the M(2) cDNA. After 48-h incubation with 1 microM carbachol or 1 microM MEG, NO production was increased by 6.5 and 6.7 fold, respectively, in cardiomyocytes overexpressing M(2) receptors. Coincubation with atropine or N(G)-nitro-L-arginine methyl ester abolished the enhancement of NO production. In contrast, NO production enhanced by carbachol or MEG in M(1)- or M(3)-transfected cardiomyocytes was similar to the level in non-transfected cells. 3. Western blot analysis showed that the protein levels of M(1), M(2), and M(3) were significantly increased in cardiomyocytes transfected with the receptor cDNAs, but MEG had no effect on the expressions. It is interesting that both carbachol and MEG caused a significant increase in constitutive endothelial NO synthase (eNOS) only in M(2)-transfected cardiomyocytes, not in non-transfected, M(1)- or M(3)-transfected cells. Again, atropine blocked the MEG-produced induction of eNOS. 4. Our data demonstrate that MEG significantly enhanced NO production in cultured cardiomyocytes and that the enhancement of NO production may result from MEG stimulation of muscarinic M(2) receptors.

Animals↗

PubMed2002

VEGF enhances functional improvement of postinfarcted hearts by transplantation of ESC-differentiated cells.

Despite considerable advances in medicine, the incidence of heart failure remains high in patients after myocardial infarction (MI). This study investigated the effects of engrafted early-differentiated cells (EDCs) from mouse embryonic stem cells, with or without transfection of vascular endothelial growth factor (VEGF) cDNA (phVEGF(165)), on cardiac function in postinfarcted mice. EDCs were transfected with green fluorescent protein (GFP) cDNA and transplanted into infarcted myocardium. Compared with the MI mice receiving cell-free medium, cardiac function was significantly improved in the MI mice 6 wk after transplantation of EDCs. Moreover, improvement of heart function was significantly greater in the mice implanted with EDCs overexpressing VEGF (EDCs-VEGF) than with EDCs alone. Frozen sections of infarcted myocardium with EDCs or EDCs-VEGF transplantation showed GFP-positive tissue. The area with positive immunostaining for cardiac troponin I and alpha-myosin heavy chain was larger in injured myocardium with EDCs or EDCs-VEGF transplantation than with medium injection. Transplantation of EDCs or EDCs-VEGF significantly increased the number of blood vessels in the MI area. However, the density of capillaries was significantly higher in the EDCs-VEGF animals than in the EDC mice. Double staining for GFP and connexin-43 was positive in injured myocardium with EDC transplantation. Our data demonstrate that engrafted EDCs or EDCs-VEGF regenerated cardiac tissue and significantly improved cardiac function in postinfarcted hearts. The novel EDCs-VEGF synergistic approach may have an important impact on future cell therapy for patients experiencing MI or heart failure.

Animals↗