Jing Gao — Page 4 | Search PubMed

PubMed2002

[Changes of thioredoxin mRNA level in neurons insulted by sodium azide].

Oxidative stress has been considered to be associated with many neurodegenerative diseases, while mitochondrial damage is one of the important reasons that aggravate oxidative stress. Sodium azide (NaN3) is a specific inhibitor of mitochondrial cytochrome c oxidase (COX), which can be used to mimic neuronal damage induced by mitochondrial deficiency. In this experiment, the neurotoxic effects of NaN3 on cultured primary neurons were detected by means of cell viability measurement (MTT assay) and morphological observation, and an in vitro model of neuronal injury induced by NaN3 were established. Further the changes of thioredoxin mRNA level in cultured neurons insulted by NaN3 were analyzed by semiquantitative RT-PCR in order to explore the role of Trx, an important redox regulatory protein, in the process of neuronal injury. It was found that cultured neurons could be damaged by NaN3 in a dose- and time-dependent manner and the expression of Trx decreased during certain dose (0-32mmol/L) and time (0-4hr) of NaN3 treatment. The above data suggest that decreased the expression of Trx may be associated with oxidative stress induced by neuronal respiratory chain injury, which could impair neuronal protective mechanism.

Animals↗

PubMedDate not supplied

Color duplex ultrasonography in detecting renal artery abnormalities in a patient with neurofibromatosis 1: a case report.

Neurofibromatosis type 1 (NF1) is a rare disease; it involves not only the nervous system, but also both large and small arteries. Common vascular complications of NF1 include arterial stenosis, aneurysms, and arteriovenous fistulas involving the abdominal aorta and its branches. A coexisting left renal artery stenosis and aneurysm in a pediatric patient with NF1 was initially detected by color duplex ultrasonography in our institute.

Aneurysm↗

PubMedDate not supplied

Transcranial color Doppler flow imaging in detecting severe stenosis of the intracranial vertebral artery: a prospective study.

OBJECTIVE: The aims of this prospective study were to investigate the manifestations of the hemodynamics and morphology of a normal intracranial vertebral artery (VA) and diffusely severe or localized critical vertebral artery stenosis (VAS) on transcranial color Doppler flow imaging (TCDFI) and to discuss the criteria of TCDFI in detecting severe VAS. METHODS: There were 30 patients suspected of having an intracranial VAS in a patient group and 30 healthy volunteers in a control group. All the patients underwent both TCDFI and digital subtraction angiography (DSA). The intracranial VA was imaged through the suboccipital window by TCDFI. The size and tortuousness of the vessels were observed. The hemodynamics of those vessels were measured by means of peak systolic velocity (PSV) and pulsatility index (PI). RESULTS: In the control group, the VA junction with basilar artery appeared as Y shaped and filled with well-distributed blue coded on color imaging, which showed flow below the baseline with fast early systolic acceleration, and normal PSV ranged from 0.42 to 0.80 m/s on pulse wave Doppler. In the patients with diffusely severe VAS (>70% lumen reduction, the length of plaque >1 cm or multiple plaques) or localized critical VAS (>90% lumen reduction) diagnosed by TCDFI and confirmed with DSA, a narrowed and tortuous VA was demonstrated with minimal flow on color Doppler imaging and decreased 50% of normal PSV and PSV <0.30 m/s accompanied by slow early systolic acceleration on Doppler spectrum. There was a significant difference (P<.05 or P<.01) in the hemodynamic parameters (PSV and PI) between patients with severe VAS and healthy subjects. CONCLUSION: TCDFI is a useful noninvasive tool in assessing the hemodynamics of intracranial arteries. The sensitivity was 90.9% and the specificity was 75.0% by using PSV <0.30 m/s and 50% reduction of normal PSV as low cutoff criteria in the diagnosis of diffusely severe or localized critical VAS with TCDFI.

Adult↗