Biomedical subjects

Jie Pu

Publications and source records attributed to Jie Pu.

3 recordsLinked to original sources

PubMed2026

Hex-MASP for mapping the whole-tissue spatial proteome and the intrabrain distribution of monoclonal antibodies.

Whole-tissue level spatial proteomics provides critical insights into region-specific biological regulations but remains challenging. Previously, we introduced the micro-scaffold assisted spatial proteomics (MASP) concept for whole-tissue mapping. However, this prototype required substantial development in spatial resolution, practicality, and throughput for practical application. Here we present a next-generation MASP technique (hex-MASP) featuring i) a new design of hexagonal-micro-wells fabricated with optimized projection micro-stereolithography 3D-printing, achieving high spatial resolution, sampling robustness, and mechanical strength for reproducibly compartmentalizing even tough tissues; ii) enhanced throughput/effectiveness in sample preparation and LC-MS analysis with high quantitative quality. Applied to mouse brain, hex-MASP achieved in-depth, whole-tissue mapping for >6,000 proteins in mouse brains, with high spatial accuracy and excellent data quality. The substantially improved resolution revealed critical regional details across the entire brain, that were not previously captured, enabling precise depiction of protein distribution heterogeneity. This technique enabled the identification of many unreported regionally enriched proteins across brain structures. We further applied hex-MASP to investigate the intrabrain distribution of intracerebroventricularly dosed antibody therapeutics and related proteins, which enabled whole-tissue mapping of protein drugs revealed insights into antibody brain penetration and distribution. Hex-MASP represents a robust, scalable platform for whole-tissue spatial proteomics.

Animals↗

PubMed2005

Up- and down-regulating facial disgust: affective, vagal, sympathetic, and respiratory consequences.

Relative to watching in a natural manner, people asked to suppress or exaggerate their facial response to a negative emotional stimulus experience greater activation of the sympathetic nervous system but report a similar subjective emotional experience. The present research extends prior research on response modulation in two important ways. First, discrete indicators of cardiac vagal and sympathetic control (respiratory sinus arrhythmia (RSA) and pre-ejection period (PEP), respectively) were included as dependent measures along with interbeat interval (IBI) and skin conductance (EDR). Second, to help generalize results across response-focused modulation techniques, participants suppressed, exaggerated, or exerted no control over their responses while watching a disgust-eliciting film (for control purposes, a fourth group was asked to watch a neutral film naturally). Response modulation was associated with significantly decreased PEP (increased cardiac sympathetic control) relative to those in the natural-watch conditions. All participants evidenced increased EDR while watching the disgusting clip, but facial modulation did not produce EDR reactivity beyond that of watching the disgusting film naturally. Exaggerators experienced decreased IBI during modulation (perhaps due to increased muscle contraction) whereas all other groups showed increased IBI (i.e., the orienting response). Neither emotional experience nor facial modulation reliably impacted RSA, respiration rate, or inspiratory depth.

Adult↗

PubMed2005

The recognition potential: semantic processing or the detection of differences between stimuli?

The recognition potential is traditionally described as an electrical index elicited when subjects view a recognizable stimulus. Recent studies further show that it may be influenced by semantic processing. In this study, we investigated whether this observed influence is really produced by differences in semantic processing or whether it might be caused by the detection of differences between sequentially presented stimuli. In two different experiments, we systematically altered the type of background images presented while keeping the recognizable word constant. Analyses revealed that the same recognizable words elicited an RP with different amplitudes and latencies when viewed under different background conditions. Control stimuli, which were identical to background stimuli, did not elicit the RP. Hence, we postulate that when using the rapid stream stimulation paradigm, RP might also be influenced by the detection of differences between sequentially input stimuli. It is necessary to clarify whether RP changes are caused by the processing of the stimuli or by the detection of difference between successively input stimuli before any conclusion could be made.

Adult↗