Biomedical subjects

Jie Li

Publications and source records attributed to Jie Li.

324 records · Page 18Linked to original sources

PubMed2002

Multiple organ dysfunction syndrome due to ingestion of fish gall bladder.

OBJECTIVE: To investigate changes in renal function, urine N-acetyl-beta-D-glucosaminidase enzyme (N-AG),liver function, myocardial enzymes, the pathology of renal damage and the mechanism of acute renal failure (ARF) associated with fish gall bladder poisoning. METHODS: Eleven patients with acute fish gall bladder poisoning were consecutively admitted to our hospital from September 1997 to October 1999. Renal function, urine N-AG enzyme, liver function, and myocardial enzymes were assayed before and after treatment. One patient consented to a kidney biopsy and the pathology of renal damage was observed under light and electron microscopes. RESULTS: All patients had multiple organ dysfunction syndrome (MODS) and 11 patients suffered from ARF. Ten patients had liver dysfunction, ten patients had poisonous myocarditis, and 8 patients had gastrointestinal dysfunction. Renal function, urine N-AG enzyme, liver function, and myocardial enzymes were significantly improved after treatment compared with those of before treatment (P < 0.05). Kidney biopsy showed that the main damage site was the proximal renal tubule. All eleven patients recovered and were discharged from the hospital. CONCLUSIONS: Ingestion of fish gall bladder leads to kidney damage, as well as liver, heart and gastrointestinal tract injury. The mechanism of acute renal function failure is the serious tubular damage, confirming the location of kidney damage. Necrosis of the proximal tubules plays an important role in the development of ARF. Immediate hemodialysis is the most effective treatment.

Adult↗

PubMed2002

[The clinical efficacy of epristeride in the treatment of benign prostatic hyperplasia].

OBJECTIVES: To estimate the clinical safety, efficacy of Epristeride in the treatment of benign prostatic hyperplasia (BPH). METHODS: One hundred and fourty-one BPH patients, aged from 50 to 83 years old [(66.18 +/- 6.41) yr], were given Epristeride tablets 5 mg p.o., twice daily for 120 days. To synthetically estimate the efficacy by the changing rate of international prostate symptom score (IPSS), maximum flow rate (Qmax), the volume of prostate (V) and the residual urine (Ru). RESULTS: The total efficacy was 81.29%. The adverse reactions include 1 case (0.72%) of exanthem, 1 case (0.72%) of nausea and vomiting, 1 case (0.72%) of insomnia, 1 case (0.72%) of tinnitus and 3 cases (2.16%) of erectile dysfunction. Two patients discontinued the treatment due to the side effects (1.44%). There was no clinical significantly abnormal laboratory index during the treatment. CONCLUSIONS: Epristeride tablets was safe and effective in the treatment of BPH.

Aged↗

PubMed2002

[Synergic effect of heparan sulfate and cytokines on in vitro growth of human umbilical cord blood hematopoietic cells].

OBJECTIVE: To explore the synergic effect of heparan sulfate (HS) and cytokines on the growth of human umbilical cord blood (UCB) hematopoietic cells. METHODS: Hematopoietic cells from human UCB were cultured with (1) cytokines (rhIL-3, rhIL-1beta, rhIL-6 and SCF) or (2) SN (supernatant from cultured human marrow stromal cells) and cytokines, or (3) SN, or (4) HS and cytokines. Cellular proliferation, CFU-GM yields and changes of cell immunophenotype were observed. RESULTS: Hematopoietic cell proliferation reached peak at the 14th day. The number of total nucleated cells increased 134.5-, 171.3-, 81.5- and 167.2-fold in (1), (2), (3) and (4) groups, respectively, at the 21th day, the (3) group significantly decreased. CD(34)(+) cells increased at the 7th day and reached peak, at the 14th day with a percentage of 68.4%, 82.5%, 69.8% and 79.3%, and at the 21th day 56.2%, 71.7.%, 12.3% and 73.3%, respectively. CD(33)(+) cells reached peak at the 14th day and increased by 80.2%, 68.6%, 81.6% and 70.3%, respectively, and remained these levels at the 21th day. CD(38)(+) cells increased by 66.6%, 73.8%, 70.4% and 71.9% at the 7th day and remained this level at the 14th and the 21th day. From the first week of culture, the percentage of CFU-GM increased in all of the four groups, at the second week of culture, it increased by 250%, 279%, 217% and 273%, and at the third week still increased by 151%, 240%, 145% and 231%, respectively. CONCLUSION: The combination of SN and cytokines have a synergic effect in promoting proliferation of hematopoietic cell from human UCB. The synergic effect remained the same when SN was replaced by heparan sulfate.

ADP-ribosyl Cyclase↗

PubMed2002

[Determination and stability tests of triclosan in disinfectants].

A method has been developed for the determination of triclosan in antibacterial detergent by RP-HPLC equipped with a diode-array detector. A Kromasil C18 column (200 mm x 4.6 mm i.d., 7 microns) was used with a mobile phase of methanol-acetonitrile-water (40:40:20, volume ratio) containing 0.02 mol/L KH2PO4 at pH 2.7 and UV detection at 280 nm. The samples were dissolved with the mobile phase by ultrasonic method. The recoveries of triclosan were from 94.2% to 102.2% and the RSDs were from 2.2% to 3.0%. The method is simple, rapid, accurate and reproducible. The concentrations of standards and samples did not change evidently after storage at 54 degrees C for 14 d and at 37 degrees C for 90 d. The results showed that triclosan has a good stability.

Anti-Infective Agents, Local↗

PubMed2002

[Relationship between trophoblast cell's human leukocyte antigen G expression and early embryo development].

OBJECTIVE: To study the expression of soluble human leukocyte antigen G (sHLA-G) in trophoblast cells of spontaneous abortion after in vitro fertilization and embryo transfer (IVF-ET) and choriocarcinoma, and explore the relationship between sHLA-G expression in trophoblast cells and the early embryo development. METHODS: 14 cases of trophoblast cells of spontaneous abortion after IVF-ET and 6 cases of choriocarcinoma tissue has been detected, and which were compared with the 10 cases of trophoblast cells of normal villus. RESULTS: (1) One case (1/14) has positive sHLA-G expression in IVF-ET group, and other cases in this group are negative or faint sHLA-G expression; ALL cases in choriocarcinoma group are strong positive sHLA-G expression; All cases in normal villous group are positive sHLA-G expression. (2) sHLA-G is expressed on the cytotrophoblast cells, especially on the extravillus cytotrophablast. The expression level of HLA-G is obviously decreased in the spontaneous abortion group compared with normal group. For single cells, the staining on the choriocarinoma cells are stronger than that of the normal villus cells. CONCLUSION: High expression of sHLA-G in choriocarcinoma trophoblast cells and in trophoblast cells of normal villus and low expression in spontaneous abortion trophoblast cells indicating sHLA-G in trophoblast cells has some relationship with the early embryo development.

Abortion, Spontaneous↗

PubMed2002

[Clinical study on treatment of HIV infected persons based on viral load detection and CD4+ T lymphocyte counting].

OBJECTIVE: To study the usage of viral load and CD4+ cell count methods in evaluating the effects of clinical treatment in HIV infected people. METHODS: Totally 73 cases of HIV infected persons were studied with clinical observation and laboratory examinations, six cases were treated with cocktail therapy, 36 cases with chinese herbs and the other 31 cases were untreated. RESULTS: The results indicated that the viral load levels in cocktail therapy group were significantly lower than the other two groups within 3 months and then kept at very low level. The CD4+ T lymphocyte levels showed a significant increase after 3 months and kept rising up. The chinese herb group, however, had no significant difference in viral load level and CD4+ T lymphocyte level as compared with untreated group. But in clinical observation chinese herbs showed the effect of improving clinical status in some cases. CONCLUSIONS: The authors conclude that the viral load and CD4+ count are efficient methods in clinical evaluation of HIV treatment, especially in routine anti-HIV therapy. As for the chinese herbs treatment, these two monitors did not reflect changes in clinical status.

Anti-HIV Agents↗

PubMed2002

[Preparation and application of monoclonal antibodies to recombinant human IFN alpha].

OBJECTIVE: To prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha). METHODS: Five cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique. RESULTS: All the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%. CONCLUSIONS: The highest purification efficiency was obtained by using 4G10 column.

Animals↗

PubMed2002

[A study on the adduct characteristics of styrene and DNA].

OBJECTIVE: To explore the adduct characteristics of styrene and DNA. METHODS: The adduct reactions between styrene, urinary mandalic acid(MA), phenylglyoxalic acid(PGA), mercapturic acid of styrene (UMA) and DNA were studied by ultraviolet spectral analysis. The SO-DNA adducts by 32P-post labeled method, the chemical structures of SO-DNA adducts by GC-MS and NMR were also studied. RESULTS: SO combined with DNA at O6, N2 positions of dGMP to form six adducts, but styrene, urinary mandalic acid, phenylglyoxalic acid and mercapturic acid of styrene did not react with DNA to form adduct. CONCLUSIONS: Styrene formed adduct with DNA through its active center metabolite--SO after entering the body. SO combined with DNA at O6, N2 positions of dGMP to form adducts. If these DNA adducts are not repaired or are mis-repaired before cell duplication, the gene mutation and chemical damage would happen. No adduct reactions are seen among other metabolites of styrene.

Acetylcysteine↗

PubMed2001

The herbicide paraquat causes up-regulation and aggregation of alpha-synuclein in mice: paraquat and alpha-synuclein.

alpha-Synuclein-containing aggregates represent a feature of a variety of neurodegenerative disorders, including Parkinson's disease (PD). However, mechanisms that promote intraneuronal alpha-synuclein assembly remain poorly understood. Because pesticides, particularly the herbicide paraquat, have been suggested to play a role as PD risk factors, the hypothesis that interactions between alpha-synuclein and these environmental agents may contribute to aggregate formation was tested in this study. Paraquat markedly accelerated the in vitro rate of alpha-synuclein fibril formation in a dose-dependent fashion. When mice were exposed to the herbicide, brain levels of alpha-synuclein were significantly increased. This up-regulation followed a consistent pattern, with higher alpha-synuclein at 2 days after each of three weekly paraquat injections and with protein levels returning to control values by day 7 post-treatment. Paraquat exposure was also accompanied by aggregate formation. Thioflavine S-positive structures accumulated within neurons of the substantia nigra pars compacta, and dual labeling and confocal imaging confirmed that these aggregates contained alpha-synuclein. The results suggest that up-regulation of alpha-synuclein as a consequence of toxicant insult and direct interactions between the protein and environmental agents are potential mechanisms leading to alpha-synuclein pathology in neurodegenerative disorders.

Animals↗

PubMed1997

Influence of Substrate and Ribozyme Sequences on the Functions of Ribozyme.

The mono-component ribozyme RZ1, RZ3 and RZ4 were ligated in different orders into two tri-component ribozymes RZ134 and RZ413.Both tri-component ribozymes could cleave individual specific substrate sequences from different regions of the tobacco mosaic virus (TMV) RNA. The cleavage efficiency and specificity of each unit-ribozymes in the tri-component ribozymes to its substrate was similar to its mono-ribozyme counterpart. When the three substrates were mixed and used as substrates for the tri-component ribozymes, only the activity of the unit-ribozymes RZ4 was clearly observed, the activities of the other two unit-ribozymes were greatly reduced. The reduction was mainly due to the fact that the two substrates BT2(+) and BT2(-) for RZ1 and RZ3 were complementary in sequence to each other and therefore formed duplex in the target region, thus must have strongly prevented the two ribozymes from forming the correct structures required for cleavage activity. In addition to its activity on the substrate BT2(+), the ribozyme RZ1 was found unexpectedly to be able to cleave BT4(-) specifically into various unique products.

Journal Article↗

PubMedDate not supplied

A prototype two-detector confocal microscope for in vivo corneal imaging.

Confocal microscopy through-focusing (CMTF) of the cornea produces a three-dimensional (3-D) display of corneal structure and intensity profiles that allow objective measurements of corneal sublayer thickness and relative assessment backscattering of light. In this study, a prototype confocal instrument was evaluated in which a photon counting photomultiplier tube (PMT) detector was added to provide faster and more quantitative measurements, while still maintaining the imaging capability of the microscope. To acquire images and measure backscattered light simultaneously, an uncoated pellicle beam splitter was incorporated into the light path of the confocal microscope. This beam splitter reflects 8% of the confocal signal to the PMT. The CMTF scans were performed on four rabbits using the prototype instrument. Corneal images and 3-D reconstructions acquired with and without the beam splitter in the light path appeared identical. Both the camera and PMT CMTF curves had easily identifiable peaks corresponding to the epithelium, basal lamina, and endothelium. No significant differences were found between PMT and camera CMTF measurements of epithelial, stromal, or corneal thickness (n = 12 scans). Furthermore, a high correlation was found between camera and PMT measurements (linear regression analysis, y = 0.999 x -0.4, r = 0.99, p < 0.001). The data suggest that by adding a pellicle beam splitter, CMTF intensity data can be acquired using a PMT. The PMT has a faster sampling rate and greater dynamic range than the camera and provides a count of the photons detected. Thus, the instrument has the potential for improving corneal pachymetry and back-scattering measurements while still providing high-resolution corneal images.

Animals↗

PubMedDate not supplied

RASSF1A promoter methylation and Kras2 mutations in non small cell lung cancer.

In the present studies, we investigated the correlation between RASSF1A promoter methylation status and Kras2 mutations in 65 primary non small cell lung cancer (NSCLC) including 33 adenocarcinomas, 12 large cell carcinomas, and 20 squamous cell carcinomas. Mutational analysis of Kras2 showed: 30% (10 of 33) of adenocarcinomas, 25% (3 of 12) of large cell carcinomas, and only 5% (1 of 20) of squamous cell carcinomas contained activated Kras2 mutation at codon 12 or 13. RASSF1A promoter region CpG island methylation was detected in adenocarcinomas (55%), large cell carcinomas (25%), and squamous cell carcinomas (25%). Interestingly, combined RASSF1A methylation and Kras2 mutation data show that only - 7% adenocarcinomas/large cell carcinomas exhibited both KRASSF1A promoter methylation and Kras2 mutation, whereas 24% adenocarcinomas, 50% large cell carcinomas, and 70% squamous cell carcinomas showed neither Kras2 mutation nor RASSF1A promoter methylation. These results showed that the majority of the primary NSCLCs with Kras2 mutations lack RASSF1A inactivation, and both RASSF1A inactivation and Kras2 mutation events occur frequently in adenocarcinomas and large cell carcinomas. Our results indicate a trend of inverse relationship between Kras2 activation and RASSF1A promoter methylation in the majority of human lung adenocarcinomas and large cell carcinomas.

Adenocarcinoma↗

PubMedDate not supplied

Antisense and dominant-negative AKT2 cDNA inhibits glioma cell invasion.

The aim of this study was to explore the potential role of AKT2 in glioma cell invasion. Therefore, dominant-negative (DN-AKT2) and antisense AKT2 constructs (AS-AKT2) were transfected into rat C6 glioma cells with elevated endogenous AKT2 expression. In situ hybridization and Western blot analysis were used to identify AKT2 expression. Spheroid culturing was used to assess cell migration and invasion in Matrigel from spheroids. Cell motility and invasion were also evaluated by scratch and Transwell invasion assays, respectively. The secretion of matrix metalloproteinases (MMPs), MMP2 and MMP9, was determined by gelatin zymography. AKT2 expression was inhibited in C6 cells transfected with AS-AKT2 but did not significantly change in cells transfected with DN-AKT2. The cell migration distance from spheroids or the number of cells migrating into the acellular space created by scratching was reduced in cells transfected with DN-AKT2 or AS-AKT2 compared to the control cells. The invasive distance of cells from the spheroids in Matrigel sandwich and the number of invading cells through the Matrigel were also decreased in the DN-AKT2- and AS-AKT2-transfected cells. Gelatin zymography showed that the production of MMP2 and MMP9 was inhibited in transfected cells. In conclusion, AKT2 plays an important role in glioma cell motility and invasion. Therapy based on AKT inhibition may complement currently available treatment to control glioma cell invasion.

Animals↗

PubMedDate not supplied

Effects of rare earth compounds on growth and apoptosis of leukemic cell lines.

To explore a new agent for inhibiting leukemic cells, we investigated the effects of rare earth compounds (lanthanum chloride and cerium chloride) on the growth and apoptosis of HL-60 and NB4 cells. The growth of HL-60 and NB4 cells was tested by 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) colorimetric assay. The apoptosis was measured by light microscopy, flow cytometry, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) method. The effect of LaCl(3) on normal bone marrow hematopoietic progenitor cells was evaluated by colony-forming unit-granulocyte-macrophage (CFU-GM) assay. Under our experimental conditions, MTT assay showed that 48-h treatment with 1, 2, and 3 mM LaCl(3) or 48- and 72-h treatments with 1 mM LaCl(3) could significantly inhibit the growth of HL-60 cells. Treatment with 2 and 4 mM CeCl(3) for 72 h could significantly inhibit the growth of NB4 cells. Apoptosis could be detected on treatment with 2 mM LaCl(3) for 24 h in HL-60 cells by light microscopic morphology examination, flow cytometric analysis, and TUNEL method. Apoptosis could be also detected on treatment with 2 mM CeCl(3) for 72 h in NB4 cells. Treatment with 1 mM LaCl(3) could arrest the transitions from G0/G1 to S phase. The granulocyte-macrophage colony formation of normal bone marrow cells was not significantly inhibited at lower concentrations of LaCl(3) (0.5 to 2 mM). Our results indicate that at certain concentrations, the rare earth compounds may inhibit the growth of leukemic cells, induce them to apoptosis, and have no significant inhibitory effects on normal bone marrow hematopoietic progenitor cells (CFU-GM). The mechanism needs to be further investigated.

Apoptosis↗

PubMedDate not supplied

Dietary glycine inhibits angiogenesis during wound healing and tumor growth.

In this study we investigated the effects of glycine on angiogenesis during embryogenesis, wound healing and tumor growth. In chorioallantoic membrane (CAM) assay, glycine (100 microM) inhibited angiogenesis by more than 50%. We studied dietary glycine's effect on fibrin induced wound healing response in a novel (Fibrin Z-chamber) assay. Fibrin within the chamber triggers the healing cascade leading to formation of granulation tissue (GT) rich in blood vessels and stroma. GT was reduced by more than 30% (p < 0.0001) in dietary Glycine groups as compared to control. We found that microvessel density dropped significantly (15%, p < 0.0003) with dietary glycine whereas the other components of GT were unaffected. We evaluated tumor growth delay utilizing Tumor Z-Chamber (fibrin with R3230 mammary adenocarcinoma cells) since tumors take advantage of angiogenesis and matrix formation. We observed that tumor growth decreased by 15% (p < 0.03) and tumor microvessel density dropped by 20% (p < 0.03) with dietary glycine compared to controls. We found that iNOS protein levels were decreased significantly in both GT (24%-57%) and tumor tissue (19-75%). In conclusion, we found that dietary glycine is a potent anti-angiogenic agent that can reduce wound healing and tumor growth through reduction of iNOS expression.

Allantois↗

PubMedDate not supplied

Octreotide augments the effects of hepatic arterial occlusion in treating a rat liver tumor model.

BACKGROUND/AIMS: To observe if octreotide can augment the effects of hepatic arterial occlusion for transplanted cancer in rat's liver. METHODOLOGY: Walker 256 carcinosarcoma was transplanted into rat's liver to create the liver cancer model. Hepatic arterial ligation was used to block the hepatic arterial blood supply. Rats bearing tumor were divided into three groups: control group, HAL (hepatic arterial ligation) group, and HAL plus octreotide group. Change of tumor volume and tumor growth inhibiting rate after therapy were evaluated. Hoechst 33342 labeling assay was used to analyze the blood perfusion of tumor (the labeled cells' number presenting blood perfusion). Expression of vascular endothelial growth factor and matrix metalloproteinase-1 mRNA was detected by in situ hybridization, and the level of serum vascular endothelial growth factor was assayed by ELISA. RESULTS: Six days after hepatic arterial ligation, the mean tumor volume in control group, HAL group, and HAL plus octreotide group was 0.103 +/- 0.043 cm3, 0.030 +/- 0.018 cm3, and 0.016 +/- 0.005 cm3, respectively. The tumor volume in the two behind groups was smaller than that in the control group (P < 0.01), and the tumor growth-inhibiting rate was 70.8%, and 84.5%, respectively. Compared with the HAL group, the tumor volume in HAL plus octreotide group decreased significantly (P < 0.05). Hoechst 33342 labeled cells' number in control group, HAL group, and HAL plus octreotide group was 369.7 +/- 30.2, 344.1 +/- 26.0, and 323.2 +/- 40.4, respectively. The number in HAL combined with octreotide group decreased significantly compared with that in control group (P < 0.05), which suggested that the blood perfusion of tumor in HAL plus octreotide group decreased significantly. The expression of vascular endothelial growth factor and matrix metalloprotenase-1 mRNA decreased slightly, but not significantly in HAL plus octreotide group compared with that in HAL group (P > 0.05). CONCLUSIONS: The results suggest that octreotide can promote the effects of hepatic arterial occlusion therapy for transplanted cancer in rat's liver. Decreasing the blood perfusion of tumor after hepatic arterial blockage maybe one of its major mechanisms.

Animals↗

PubMedDate not supplied

Expression of p63 mRNA isoforms in normal human tissue.

This report describes the presence of different p63 isoforms in normal human tissue. The p63, expressed by basal cells of stratified epithelia and myoepithelial cells, is characterized by different mRNA isoforms formed by alternative splicing. Three isoforms were shown to act pro-apoptotically, while the remaining three isoforms are anti-apoptotic. Therefore a shift in a balance between different p63 isoforms might promote or inhibit normal and malignant growth. Our results show that in bone marrow, brain, colon, skeletal muscle, spleen and testis only pro-apoptotic p63 isoforms are expressed, whereas only anti-apoptotic isoforms are present in lung, placenta and salivary gland. In kidney, prostate, skin, thymus, trachea and uterus both variants are present. Presented results will allow in the future to compare p63 isoforms in various pathological processes with normal tissue pattern in order to evaluate the contribution of p63 isoform imbalance to the pathophysiology of various malignant diseases.

Alternative Splicing↗

PubMedDate not supplied

Transient increased expression of VEGF and MMP-1 in a rat liver tumor model after hepatic arterial occlusion.

BACKGROUND/AIMS: This study investigates the influence of hepatic arterial occlusion (HAO) on blood perfusion of transplanted cancer in rat's liver, and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP-1) and explores the mechanisms involved in transcatheter arterial embolization (TAE)-induced metastasis of liver cancer preliminarily. METHODOLOGY: Walker 256 carcinosarcoma was transplanted into rat's liver to create the liver cancer model. Hepatic arterial ligation (HAL) was used to block the hepatic arterial blood supply and simulate TAE. Rats bearing tumor were divided into three groups: control, laparotomy control, and HAL groups. Blood perfusion of tumor was analyzed by a Hoechst 33342 labeling assay. The level of serum VEGF was assayed by ELISA; and the expression of VEGF and MMP-1 mRNA was detected by in situ hybridization. RESULTS: Two days after HAL, the number of Hoechst 33342 labeled cells which represent the blood perfusion of the tumor directly and hypoxia of tumor indirectly in the HAL group decreased significantly compared with that in the control group (329.1+/-29.3 vs. 383.6+/-19.2, P<0.01). The level of serum VEGF in the HAL group increased significantly compared with that of the control group (92.5+/-43.9 pg/mL vs. 54.9+/-19.3 pg/mL, P<0.05). The expression of VEGF and MMP-1 mRNA in the tumor tissue of the HAL group increased significantly compared with that of the control and the laparotomy control groups (P<0.05). The blood perfusion data of the tumor, represented by number of Hoechst 33342 labeled cells, showed an inverse correlation with the expression of VEGF mRNA in tumor tissue (P<0.05). While 6 days after HAL, the blood perfusion of tumor in HAL group decreased and the expression of VEGF and MMP-1 increased only slightly, not significantly, compared with that in the control group. CONCLUSIONS: In conclusion, blockage of hepatic arterial blood supply results in transient decreased blood perfusion and increased expression of metastasis-associated genes VEGF and MMP-1 of transplanted liver cancer in rats. Decreased blood perfusion and hypoxia may be the major reason for up-regulated expression of VEGF. Better understanding of the mechanisms involved with TAE-induced metastasis may lead to the enhancement of the long-term effects of TAE for liver cancer.

Animals↗