Search PubMed⌕ Search

Biomedical subjects

Jie Chen

Publications and source records attributed to Jie Chen.

At least 163 records · Page 9Linked to original sources
PubMed2005

Water-soluble antioxidants improve the antioxidant and anticancer activity of low concentrations of curcumin in human leukemia cells.

Curcumin (Cur) is a promising antioxidant and anticancer drug, but several recent studies indicate that Cur exerts its anticancer activity through promoting reactive oxygen species (ROS) generation. In the present study, concentration-dependent regulation of Cur on cell proliferation, viability and ROS generation, and effect of water-soluble antioxidants ascorbic acid (ASA), N-acetyl-cysteine (NAC) and reduced glutathione (GSH) on the antioxidant and anticancer activity of Cur were investigated in human myeloid leukemia cells (HL-60 cells). We found that although Cur concentration- and time-dependently decreased the proliferation and viability of cells, its effect on ROS generation (as indicated by the level of malondialdehyde, MDA) varied with its concentrations. I.e., low concentrations of Cur diminished the ROS generation, while high Cur promoted it. Combined with the opposite effect of 50 microM H2O2 on low or high Cur-induced MDA alteration, cell proliferation arrest and cell death, these results proved that low Cur exerted its anticancer activity through diminishing ROS generation in HL-60 cells, while high Cur through promoting ROS generation. Further studies showed that all water-soluble antioxidants ASA, NAC and GSH significantly enhanced both the antioxidant and the anticancer activity of low Cur. Considering that the extra accumulation of ROS is harmful to normal cells, the data presented here indicate that instead of using high doses, combining low doses of Cur with water-soluble antioxidants is a better strategy for us to improve the anticancer activity of Cur.

Acetylcysteine↗
PubMed2005

[Analysis of gene expression information in the fat and muscle tissues of pig].

An in silico study was developed to detect the gene expression profile of pig fat and muscle tissues by using the porcine EST resources and human gene sequences,and thus provided candidate information for basic genetic analysis in meat quality improvement of pig. In this study,a BLAST search was performed to identify homologies between the cDNA sequences of human genes and the ESTs sequences of pig,and the high homologous records were screened out. Four Java programs were developed to retrieve and collect sequences, and analyze the BLAST alignment results. By statistical analysis, it was found that there were at least 2002 genes expressed in the fat and muscle tissues of pig, and 1087 in the fat tissue, 1205 in the muscle respectively (290 genes co-expressed in the two tissues). The top-ranking records were screened out,and meantime, 114 basic active genes (BAGs) were found to express in the two tissues,80 in the fat tissue and 34 in the muscle tissue respectively. The top 10 records were described in the paper. This study was summarized in relation to current meat quality improvement of pig at molecular level.

Adipose Tissue↗
PubMed2005

[Antitumor effect of epidermal growth factor receptor gene silencing].

OBJECTIVE: To investigate whether chemically synthesized double-stranded RNA (dsRNA) targeting epidermal growth factor receptor (EGFR) could induce gene silencing in non-small-cell lung cancer (NSCLC) cells, and to assess the degree of EGFR gene silencing and its effect on functional outcome. METHODS: NSCLC cell line SPC-A-1 was transfected with target sequence-specific dsRNA formulated with Lipofectamine 2000. Fluorescent microscopy and flow cytometry were used to measure the reduction in the production of the EGFR protein. Real-time PCR was used to detect the silencing of the EGFR gene level. Colony assay was adopted to measure the cellular proliferation and colony formation. A tumor burdened athymic nude mouse model was established to calculate the tumor growth inhibition rate. RESULTS: The dsRNA-EGFR was shown to be effective with a 71.3% down-regulation of EGFR protein production and 50.0% of silencing of EGFR gene. The dsRNA-EGFR significantly reduced colony numbers by 66.8% in vitro and inhibited the tumor growth in vivo. The tumor growth inhibition rate was 75.0%. CONCLUSION: The sequence specific dsRNA showed a blockbuster effect in downregulation of EGFR gene level and protein production, inhibition of the cellular proliferation and tumor growth.

Animals↗
PubMed2005

[Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with different metastasis potentials].

OBJECTIVES: To compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence. METHODS: Using two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials. RESULTS: 10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I. CONCLUSION: The changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.

Carcinoma, Hepatocellular↗
PubMed2005

[Dexamethasone pharmacokinetics in perilymph of guinea pig after different topical administrations].

OBJECTIVE: To determine pharmacokinetics profiles of dexamethasone (DEX) in perilymph of guinea pig with different intratympanic administrations, and to provide experimental evidence for clinical choice about administration. METHOD: Two different topical administrations placing a granule gelfoam soaked with DEX (10 g/L) in the niche of round window of guinea pig and infusing tympanic bulb with DEX solution (10 g/L) were conducted. Perilymph of the scala tympani was sampled 1-6 h after administration. DEX in the samples was assayed with high-performance liquid chromatography. RESULT: The concentrations of DEX in perilymph after placing a granule gel-foam soaked with DEX in the niche of round window were significantly higher than that after intratympanic infusing. The half-life time of DEX after placing a granule gelfoam was longer. CONCLUSION: DEX could effectively penetrate round window membrane into perilymph after different intratympanic administrations, and the method of placing a granule gelfoam soaked with DEX in the niche of round window is more effective than that of intratympanic infusing.

Administration, Topical↗
PubMed2005

[Effects of microsatellite DNA markers on meat quality traits in pig chromosome 13].

In this reseanch, 7 microsatellite DNA loci linked with PPAR gene were selected from the published genetic map of chromosome 13 in pig,and polymorphisms of these microsatellites in 100 samples from Sutai pigs (Duroc x Erhualian) populations were detected. Results revealed that the number of alleles were 6-9, heterozygosity 0.59 - 0.81, polymorphism information content 0.51 - 0.76. Effects of S0021, SW1937, SW482, S0222, S0293, S0281 and SWR2054 on meat quality traits were analyzed with PROC GLM of SAS. Results showed that the effects of S0021 on pH value and SW937 on water-holding capacity reached a significant level at P < 0.01 respectively. The effect of S0293 on tenderness and SW482 on BFT were also significant (P < 0.05). S0222, S0281 and SWR2054 had no significant effect on the 7 selected meat qualitytraits (P>0.05).

Alleles↗
PubMed2005

[CD4+ and CD8+ T cells in gastric mucosa in children infected with Helicobacter pylori].

OBJECTIVE: To study the changes of gastric mucosal CD4(+) and CD8(+) T cells in Helicobacter pylori (Hp) infected children. METHODS: Seventy nine patients with digestive tract symptoms were assessed by endoscopy, rapid urease test and histology. Forty four patients had Hp positive chronic superficial gastritis (Hp(+)CSG) and 35 patients had Hp negative chronic superficial gastritis (Hp(-)CSG). Gastric biopsy specimens were obtained from each patient. Peripheral blood samples were obtained from 33 patients (12 with Hp(+)CSG, 21 with Hp(-)CSG). Hp infection was identified by rapid urease test and histology. Hp infection was confirmed when a patient was positive for both of these tests. Four pieces of gastric antrum mucosal specimens were placed in Hank's balanced salt solution containing 1 mmol/L dithiothreitol (DTT) and 1 mmol/L ethylenediamine tetraacetic acid (EDTA). The specimens were treated with collagenase type I (120 U/ml) for three hours at 37 degrees C with agitation. The mononuclear cells were collected by removing undigested material and washed three times with RPMI 1640. Isolated gastic mononuclear cells were stained with CD3-FITC (fluorescein isothiocyanate), CD4-PE (R-phycoerthrin), CD8-PerCP (Peridinin-chlorophyll-alpha-protein) and measured by flow cytometry. Mucosal T lymphocytes were gated for the expression of CD3. Peripheral blood lymphocyte subsets were analysed by direct immunofluorescence. RESULTS: The percentage of isolated gastric mononuclear cells within the CD3 gate were 3.26 +/- 1.98 in Hp(-)CSG, 4.37 +/- 1.97 in Hp(+)CSG. Relative CD4(+)(%), CD8(+)(%) and CD4(+)/CD8(+) of the CD3(+) cells respectively were 23.74 +/- 10.37, 47.04 +/- 12.00, 0.52 +/- 0.23 in Hp(-)CSG group, 40.28 +/- 11.35, 27.91 +/- 8.84, 1.55 +/- 0.52 in Hp(+)CSG group. CD4(+)(%), CD4(+)/CD8(+) in Hp(+)CSG group were significantly higher than those of Hp(-)CSG group and CD8(+)(%) was lower than those of Hp(-)CSG group (P < 0.01). There were no significant differences in peripheral blood T lymphocyte subsets between the two groups. CONCLUSION: The difference of gastric T lymphocyte response between Hp(+)CSG and Hp(-)CSG in children indicated that the local cellular immune reaction may play a critical role in the pathogenesis of Hp infection.

Biopsy↗
PubMed2005

[The control rate of irbesartan/hydrochlorothiazide combination regimen in the treatment of Chinese patients with mild to moderate hypertension].

OBJECTIVES: To analyse the control rate of irbesartan/hydrochlorothiazide (HCTZ) combination tablets (COAPROVEL) in the treatment of patients with mild to moderate primary hypertension. METHODS: In this multi-center, open, single therapy trial, the enrolled patients aged 18-75 were treated with irbesartan/HCTZ combination tablets for 8 weeks. The initial dose comprised one tablet of irbesartan (150 mg)/HCTZ (12.5 mg) once a day during the first 2 weeks. If diastolic blood pressure was greater than 85 mm Hg at the end of the second or fourth weeks, irbesartan (300 mg)/HCTZ (12.5 mg) once a day or irbesartan (300 mg)/HCTZ (25 mg) once a day were added respectively. RESULTS: In 968 patients with mild to moderate hypertension enrolled, 920 patients were followed up for 8 weeks. (1) After 1 week of treatment, irbesartan/HCTZ combination tablets lowered systolic blood pressure by 11.87 mm Hg and diastolic blood pressure by 8.54 mm Hg (P < 0.01). After 8 weeks of treatment, the corresponding decreases were 21.97 mm Hg and 16.08 mm Hg, respectively (P < 0.01). (2) After 2, 4 and 8 weeks of treatment, 526, 703 and 769 patients reached blood pressure target (diastolic blood pressure less than 85 mm Hg). The control rates were 57.17%, 76.41% and 83.59%, respectively. (3) Among the 920 patients who completed the trial, 637 patients took irbesartan (150 mg)/HCTZ (12.5mg) once a day (69.24%), 211 patients took irbesartan (300 mg)/HCTZ (12.5 mg) once a day (22.93%), and 72 patients took irbesartan (300 mg)/HCTZ (25 mg) once a day (7.82%). (4) In the intention-to-treat analysis, no adverse reaction was observed in 903 patients (93.29% of the patients enrolled). CONCLUSIONS: When irbesartan/HCTZ combination regimen are used in the treatment of patients with mild to moderate primary hypertension, the proportion of patients reaching blood pressure target is high and adverse reactions are rare.

Adolescent↗
PubMed2005

[Study on patient satisfaction with two type of Twin-block].

OBJECTIVE: To compare patient satisfaction with two type of Twin-block by clinic complain questionnaire, treatment effect satisfaction questionnaire and total satisfaction questionnaire, and to analyse their related factors with patient satisfaction. METHODS: 56 patients were randomly classified into fixed-Twin-block (TB1) and unfixed-Twin-block (TB2). The complaint and satisfaction of patients was evaluated by clinic complain questionnaire, treatment effect satisfaction questionnaire and total satisfaction questionnaire after 10 days and 6 months of treatment. RESULTS: (1) There was significant difference between the complaint of two groups in the sixth month, but there was no difference in the tenth day. (2) There was significant difference between the total satisfactory degree of two groups. (3) There was significant difference between the tooth-jaw effective index and the mandible effective index of the two groups. CONCLUSION: The satisfactory degree of fixed-Twin-block is superior to that of unfixed-Twin-block. The adorn fashion, treatment stage and functional position has influence on patient satisfaction.

Female↗
PubMed2005

[Influence of different porcelain surface treatment method on the bonding of metal brackets to porcelain].

OBJECTIVE: To investigate the influence of different porcelain surface treatment methods on the shear bond strength of metal brackets bonded to porcelain. METHODS: 80 porcelain facets were divided randomly into two groups according to different adhesive material that was used to bond metal brackets. Adhesive material were Jing-Jin enamel adhesive and light-cured composite resin. Each group was further divided into 4 subgroups according to different surface treatment methods, which were acid etching with 37% phosphoric acid (H3PO4), acid etching with 9.6% hydrofluoric acid (HF), deglazing by grinding and silanating the porcelain surface. All specimens were stored in 37 degrees C water for 24 hours and then the shear bond strength and the porcelain fracture after debonding was determined. The porcelain surfaces after HF etching, H3PO4 etching and deglazing by grinding were examined by scanning electron microscopy respectively. RESULTS: The shear bond strengths in the HF etching groups, the deglazing groups and the silanating groups were much greater than that in the phosphoric etching groups (P < 0.01). Adequate orthodontic bonding strength was achieved both when bonded with light-cured composite resin after deglazing by grinding and when bonded with either of these adhesives after HF etching or surface silanating. There were no differences in the rates of porcelain fractures among groups (P > 0.05). CONCLUSION: HF etching, deglazing by grinding and silanating can all increase the shear bond strength between metal bracket and porcelain. Surface silanating of porcelain is a better surface treatment when metal brackets bonded to porcelain.

Composite Resins↗
PubMed2005

[Olfactory acuity and improvement of olfaction after total laryngectomy].

OBJECTIVE: To test and evaluate the olfactory function of patient after total laryngectomy, seek to a practical method to ameliorate olfactory function and rise the qualitative character of these patients. METHODS: Using the T&T olfactory examination to evaluate the olfactory function of 60 cases. Four cases olfactory mucosae were observed by electron microscope. Observing relation among the remains olfaction, the time after operation and whether or not undergone the voice reconstruction. And analyse the reasons of the above hyposomnia. Using the closing-mouth and nasal out-word airflow maneuver (CNOAM) as the intervention in the patients of tracheoesophageal fistula voice reconstruction after total laryngectomy to observe the amelioration after intervention. RESULTS: It shows various degree of hyposmia and anosmia in the cases after total laryngectomy with or without tracheoesophageal fistula voice reconstruction, with significant deference (P < 0.01) compared to the normal person respectively. There are precisely correlation among the time after operation and whether or not undergone the voice reconstruction. The longer time leads to less remaining olfaction. The patients after total laryngectomy without tracheoesophageal fistula voice reconstruction have lost their olfaction thoroughly within 5 years. But for the patients after total laryngectomy with tracheoesophageal fistula voice reconstruction, they have a middle hyposmia within 5 years, with significant deference (P < 0.01) between the patients in 5 years and after 5 years. There were significant differences (P < 0.01) between the values of patients with and without tracheoesophageal fistula voice reconstruction. The ultrastructure of 4 cases of olfactory epithelium shows the apoptosis change. After the treatment of CNOAM, the remaining olfaction of most patients were improved, with significant deference (P < 0.01) compared to those before the treatment of CNOAM. CONCLUSIONS: The proceed hypofunction of olfaction may be influenced by the reform of respiratory air, the extinction of air velocity bypass the nasal cavity and the apoptosis of epithelial cells in the patients after total laryngectomy. But if we give an early intervention study such as tracheoesophageal fistula voice reconstruction and CNOAM, the olfactory function may be maintenance. During the intervention, the ending of olfactory nerves may be get uninterrupt stimulation. This may help the patients keep a better existing quality than those fail to accept the interventions.

Adult↗
PubMed2005

[Studies on the triterpenoids of Cyclocarya paliurus (Batal.) Iljinsk].

Three triterpenes (I-II) were obtained from the leaves of Cyclocarya paliurus (Batal.) Iljinsk. By means of physicochemical and spectral methods, the structures of the three triterpenes were identified as oleanolic acid (I), ursolic acid (II) and epikatonic acid (III) respectively. All of the three triterpenes were isolated for the first time from the plant of Cyclocarya paliurus (Batal.) Iljinsk.

Juglandaceae↗
PubMed2005

[Effect of neferine on the chemotherapic sensitivity of STI 571 to K562/A02 cells].

OBJECTIVE: To explore the effect of neferine on the chemotherapic sensitivity of STI 571 to K562/A02 cells and to reveal its mechanism. METHODS: MTT method was used to observe the alteration of the proliferation of K562/A02 cell line treated with STI571 alone or combined with neferine. The transcription of mdrl gene was detected by semi-quantitative RT-PCR and the P-gp expression was determined by Western blot after STI 571 alone or combined with neferine treatment. RESULTS: The cytotoxic effect of STI 571 (1 micromol/L) combined with neferine (IC50 = 3.02 micromol/L) on K562/A02 cell line was significantly higher than that of STI 571 alone (IC50 = 0.689 micromol/L). After treating with STI571 combined with neferine, the synergistic interaction on K562/A02 cells increased 4.38 folds (P < 0.05); the mdrl mRNA expression by semi-quantitative RT-PCR was significantly reduced by (45.4 +/- 2.5) % (P < 0.01); and the P-gp expression by Western blot was deregulated by 40.58% (P < 0.05). CONCLUSION: Neferine significantly enhances the antineoplastic effect of STI 571 on K562/A02 cells by reducing mdrl mRNA transcription and blocking P-gp expression.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed2005

Quercetin and trichostatin A cooperatively kill human leukemia cells.

Quercetin (QU) and trichostatin A (TSA) are promising anticancer drugs. While QU mainly exerts its anticancer activity through scavenging reactive oxygen species (ROS), the anticancer activity of TSA was attributed to its inhibition on histone deacetylases (HDAC). In the present study it was investigated, whether the combination of QU and TSA could improve their anticancer activity against human leukemia cells (HL-60). The cytotoxicity of QU and TSA increased in a time and dose-dependent manner. QU (10, 20 and 40 microM) was able to diminish the ROS generation (indicated by the level of malondialdehyde, MDA) but showed no influence on the histone acetylation in HL-60 cells; on the contrary, TSA (20, 40, 80 and 160 nM) showed no inhibition on ROS generation but significantly increased the histone acetylation, indicating the possible role of both scavenging ROS and increasing histone acetylation in the induction of cell death in HL-60 cells. This conclusion was confirmed by the findings that the combinations of QU and TSA at different concentrations could not only diminish ROS generation, but also increase histone acetylation, and hence showed more significant cytotoxicity in HL-60 cells than either of its components. Collectively, the present data indicate that a combination of QU and TSA can cooperatively kill HL-60 cells through the combination of their activities of scavenging ROS and increasing histone acetylation.

Acetylation↗