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Biomedical subjects

J del Pino

Publications and source records attributed to J del Pino.

6 recordsLinked to original sources
PubMed1995

Heterogeneous decrease of bone mineral density in the vertebral column of ovariectomized rats.

The long-term effect of ovariectomy on the loss of bone mineral density (BMD) was evaluated in rats with and without estrogen treatment; BMD was studied in the lumbar and caudal vertebrae, measured by DXA, to find how the losses of BMD occur in the axial skeleton. Seventy female Wistar rats of 3 months of age were divided into four groups as follows: group 1: control animals; group 2: ovariectomized animals; group 3: ovariectomized animals undergoing treatment with estrogen (0.25 mg/kg per week of 17-beta estradiol); group 4: ovariectomized rats undergoing estrogen treatment only during the last 3 months of the experimental period. No significant differences were found among the groups in regard to the BMD values of the caudal vertebrae at either 3 or 6 months. Likewise, in the lumbar vertebrae there were no significant differences among the groups after 3 months. However, at 6 months, a decrease in the BMDs of the ovariectomized animals with respect to the remaining groups was found: 226 +/- 11 mg/cm2 in the ovariectomized group; 262 +/- 14 mg/cm2 in the controls; 255 +/- 4 mg/cm2 in the rats receiving estrogen treatment for 6 months; and 259 +/- 5 mg/cm2 in the animals receiving estrogen for 3 months. The study also reveals the absence of differences in the bone mineral density between the ovariectomized and control rats when the former received estrogen treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

Absorptiometry, Photon↗
PubMed1991

Potentiation of cocaine hepatotoxicity by ethanol in human hepatocytes.

The hepatotoxic effects of cocaine on the human liver and the effect of ethanol on cocaine-induced hepatotoxicity have been examined in adult human hepatocytes cultured in chemically defined conditions. Cultures were exposed to concentrations of cocaine ranging from 10(-2) to 10(-5) M. Cytotoxicity was evaluated after 24 hr of continuous exposure to cocaine by measuring the leakage of intracellular LDH and the ability of cells to reduce MTT. According to these end-point parameters, half-maximal cytotoxic concentrations of cocaine for human hepatocytes (IC50) were 6.8 and 7.8 mM, respectively. Lower concentrations of cocaine, however, impaired basic metabolic functions of human hepatocytes. Exposure of cells to 2 mM cocaine for 24 hr resulted in a 50% decrease in hepatic glycogen, a 40% decrease in cellular glutathione content, and a 40% decrease in urea synthesis with respect to control values. For most of the metabolic parameters assayed, significant alterations were observed at 0.5 mM cocaine. Glycogen reloading of hepatocytes began to be inhibited in the presence of 0.60 mM cocaine (IC10). Ethanol greatly potentiated cocaine-induced hepatotoxicity. After a 48 hr pretreatment of human hepatocytes with 50 mM ethanol, low concentration of cocaine (0.25 mM) that had no effects on hepatocyte metabolism in the absence of ethanol caused a 20% inhibition of the urea synthesis rate, a 40% degradation of glycogen stores, and a 30% reduction in glutathione content. The results of our work show that ethanol increases the effects of cocaine on human hepatocytes by a factor of 10.

Cells, Cultured↗
PubMed1990

Influence of sex, age, and menopause in serum osteocalcin (BGP) levels.

Osteocalcin (bone Gla protein) is a promising marker of bone turnover useful in the diagnosis and follow-up of high turnover osteoporosis. Conflicting results have been reported about its physiological variations according to sex, age, and menopause. Several, but not all, authors have found increased levels in males, with aging, and after menopause. We measured serum osteocalcin in 126 healthy subjects, 57 males and 69 females, aged between 45 and 88 years. Osteocalcin was higher (P less than 0.01) in males (6.24 +/- 0.36) than in females (4.32 +/- 0.34). This sexual difference was significant, too, in subjects younger and older than 60 years. Osteocalcin increased with age, linearly in males (P less than 0.05), and exponentially in females (P less than 0.05). Although there was a difference in age (P less than 0.05), no difference in osteocalcin levels between premenopausal women and women in their first two postmenopausal years was detected, while osteocalcin was significantly increased in women more than two years into menopause. We conclude that osteocalcin in healthy subjects is higher in males than in females and increases with age after 45 years in both sexes. Osteocalcin levels increase in women more than two years beyond menopause, but not only as an effect on aging.

Age Factors↗
PubMedDate not supplied

Influence of clinical and immunological parameters on the health-related quality of life of patients with primary Sjögren's syndrome.

OBJECTIVE: To evaluate health-related quality of life (HR-QoL) in patients with primary SS patients using the SF-36 questionnaire and to analyse the association between the main clinical features and the SF-36 scales. METHODS: We studied 110 patients (105 women and 5 men, mean age of 56 years) with primary SS seen consecutively in the outpatient clinic of our Department. We used the population-based reference values for the Spanish version of the SF-36 health survey as control values for a healthy population. RESULTS: Comparison between patients with primary SS and the control population showed lower scores in all SF-36 scales (p < 0.001). Analysis of the SF-36 scales by gender showed a significant correlation between age and the values for physical functioning (p = 0.013) and bodily pain (p = 0.016) scores. No significant differences in SF-36 scores were found when comparing patients according to the presence or absence of sicca features. Women with vaginal dryness had lower scores for social functioning (61.9 vs. 74.4) and general health (37.2 vs. 44.7) than those without, although the differences were not statistically significant (p > 0.05). Patients with extraglandular involvement had lower scores for the vitality scale (40.8 vs. 54.5 p = 0.007), social functioning (67.0 vs. 79.8, p = 0.010), bodily pain (49.5 vs. 62.5, p = 0.018) and general health (38.6 vs. 49.4 p = 0.001) than those without. CONCLUSION: Patients with primary SS had clearly lower HR-QoL scores than the healthy population; with significantly lower scores in all SF-36 scales and in both summary measures. We identified several epidemiological and clinical SS features related to these lower SF-36 scores. Age at protocol correlated with physical functioning and bodily pain. Vaginal dryness was the sicca feature that most affected the HR-QoL of female SS patients, and a poor HR-QoL was also observed in those patients with a systemic expression of the disease, with pulmonary involvement being the extraglandular manifestation that most contributed to a poor HR-QoL. Our results highlight the importance of earlier diagnostic and therapeutic management of patients with primary SS, which, together with a close follow-up, may contribute to a significant improvement in their HR-QoL.

Female↗