Search PubMed⌕ Search

Biomedical subjects

J Zhou

Publications and source records attributed to J Zhou.

At least 469 records · Page 26Linked to original sources
PubMed1999

[Clinical observation of side effects of Tripterygium preparation].

OBJECTIVE: To observe the side effects of Tripterygium preparation (TP) in the course of treatment. METHODS: Two hundred and seventy-one cases of patients with rheumatoid arthritis (RA) were treated using various TP to observe the occurrence of adverse reaction. RESULTS: The influence on reproductive system with TP extracted from the leaf ester tab were less than that from the root (TP tab and TP multiglycoside tab, TPMG). In group of TPMG the rate of side effect of 60 mg daily dosage was more than 30 mg daily (P < 0.01). The main influence on digestive tract and irregular menstruation was before age of 50 years and on renal function was after age of 50 years. Those adverse reactions mainly occur within first ten years in long therapy period. The side effects of TPMG produced in the Dampness-Heat type of RA were lower than Yin-Deficiency of Liver and Kidney type of RA (P < 0.05). CONCLUSION: TP ester tab is available for young woman and TPMG tab is suitable for the Dampness-Heat type of RA with lower dosis maintenance therapy.

Adult↗
PubMed1999

[Hepatic resection with removal of tumor thrombi for hepatocellular carcinoma with tumor thrombi in portal vein and curative analysis].

OBJECTIVE: To study the therapeutic effects of surgical treatment for hepatocellular carcinoma (HCC) with tumor thrombi in the main trunk or the first branch of the portal vein (PVTT) and factors affecting prognosis. METHODS: 111 HCC patients with PVTT underwent hepatic resection with removal of tumor thrombi in the first left or the right branch of the portal vein or removal of tumor thrombi by direct opening of the main trunk of the portal vein. Hepatic artery infusion and/or portal vein infusion were performed after hepatic resection with removal of tumor thrombi for HCC with PVTT in 22 of the patients. Among 111 patients, 32 received postoperative transhepatic arterial chemoembolization and/or portal vein chemotherapy. Conservative treatment and surgical exploration or hepatic artery ligation (HAL) and infusion (HAI) and/or portal vein infusion (PVI) were performed in other 14 and 20 HCC patients with PVTT, respectively. RESULTS: The 1-, 2-, 3-, 4- and 5- year survival rates were 61.7%, 36.2%, 32.3%, 24.4% and 22.4% in the resected group, respectively, whereas 14 HCC patients with PVTT treated conservatively died in three months and the 1-, 2- year survival rates were 6.0% and 0 in the 20 patients with surgical exploration or HAL and HAI and/or PVI respectively. CONCLUSIONS: Hepatic resection with removal of tumor thrombi for HCC with PVTT should be encouraged for the prolongation of life span and quality of life.

Adult↗
PubMed1999

[Plasma thyroid hormones during cardiopulmonary bypass].

OBJECTIVE: To study the changes and clinical significance of plasma thyroid hormones were during cardiopulmonary bypass (CPB). METHODS: Plasma thyroid hormones was studied in 36 patients during open heart surgery under CPB. Blood samples were taken preoperatively, and 1, 2, 3, 4, 5 days postoperatively. Doppler echocardiography was performed in 16 patients before and after surgery for evaluating heart function. RESULTS: Before CPB, the levels of thyroid hormones were within normal range. At early stage after CPB, the levels of FT(3), FT(4), TT(3) and TT(4) were significantly decreased and increased. Both TSH and rT(3) were increased significantly after CPB (P < 0.05) and reached their highest levels at the first postoperative day. TSH and rT(3) were gradually dropped subsequently, but TSH returned to the basic level and the level of rT(3) was still high. A positive relation was seen between plasma thyroid hormones and cardiac function. CONCLUSIONS: The changes of plasma thyroid hormones are closely related to cardiopulmonary bypass and could result in postoperative cardiac disfunction.

Adult↗
PubMed1999

[A study on relationship between blood lead level and physical growth and development of babies and young children in Shanghai].

OBJECTIVE: To study the effects of low level lead exposure on physical growth and development in young children. METHODS: Blood lead level and indicators for physical growth and development, such as body height and weight, and head and chest circumferences were measured in randomly sampled 1,969 children aged 1 - 6 years in Shanghai. Z scores for these indicators were calculated for each of them based on their age and sex-specific norms for children in urban and suburban Shanghai. And, regression analysis of blood lead level was conducted on them. RESULTS: Geometric mean of blood lead level was 0.400 micromol/L in young children of Shanghai, and 744 (37.8%) of 1,969 children were equal to or higher than 0.483 micromol/L. Geometric mean of blood lead level correlated in reverse to the Z scores for their body height, weight and head circumference, even if adjusted for the potential confounding factors. CONCLUSION: Low level lead exposure could cause adverse effects on their physical growth and development in young children of Shanghai.

Body Height↗
PubMed1999

[Hemorrheological status in patients with silicosis and its clinical significance].

OBJECTIVE: To study relationship between silicosis and hemorrheology and its clinical significance. METHODS: Varied indices for hemorrheology were measured in 85 male patients with silicosis, 45 male patients with chronic bronchitis and 100 healthy male subjects. RESULTS: Compared with the control group and the chronic bronchitis patient group, average hematocrit (Hct), whole blood apparent viscosity (eta(a)) at 4 s(-1) - 200 s(-1) shear rates, plasma viscosity (eta(p)), erythrocyte electrophoresis time (EET) and platelet electrophoresis time (PET) all significantly increased in patients with silicosis (P < 0.001). Hemorrheological indices in patients with silicosis increased with severity of disease, increased with decrease in their pulmonary function, and were all significantly higher than those in the control group (P < 0.01 approximately equal 0.001). Stepwise regression analysis showed that severity of disease correlated closely to Hct, eta(a) at 4 s(-1) - 200 s(-1), EET and PET, and that pulmonary function status in patients with silicosis and their courses correlated closely to Hct, eta(a) at 4 s(-1) - 200 s(-1), eta(p), EET and PET. CONCLUSIONS: Blood of patients with silicosis was in highly viscose status, which was easy to be complicated with multiple diseases.

Aged↗
PubMed1999

[Molecular typing of staphylococcus aureus with randomly amplified polymorphic DNA].

The randomly amplified polymorphic DNA(RAPD) was used to type the 26 clinically isolated strains of staphylococcus aureus. The results were that all three primers of this method, ERIC-2, Ap1, and Ap7 on molecular typing of staphylococcus aureus and identified 9, 6, and 10 genotypes, respectively; the primer Ap7 produced better results than the other primers. The present study suggests that RAPD is a simple and rapid method and has a practical value in the molecular typing of staphylococcus aureus.

Polymerase Chain Reaction↗
PubMed1999

[Relationship between smoking and drinking and herniation of intervertebral disc].

To study whether there is relationship between smoking and drinking and herniation of intervertebral disc, we investigated 214 patients who had been proved with herniation of intervertebral disk during 1986-1996. The results were that in the younger (< or = 35 year old), no matter whether they drank or not, the number of smoker in the patients with herniation of intervertebral disc was more than that in the control group; no matter whether they smoked or not, the number of drinker in the patients with herniation of intervertebral disc was more than that in the control group.

Adolescent↗
PubMed1999

[Hip ambulatory abduction in the treatment of Legg-Calve-Perthes disease].

Forty-eight cases of Legg-Calve-Perthes disease were treated with hip ambulatory abduction brace. The children were able to walk, sit, climb, or squat with the brace. Thirty-nine cases were followed up more than three years. The total excellent rate was 82%. The result of Catterall Grade I-II cases was all excellent. The excellent rate was 80% below five-year-old cases of Catterall III-IV during the start of the treatment and 66.6% above five. With the active hip abduction and internal rotation, the femoral head was held well. The results show that the method is simple and with no operation and with satisfactory effect of the treatment.

Braces↗
PubMed1999

[Effect of acrylonitrile on the activity of Ca(2+)-ATPase and phosphorylase A of liver in rats].

The activity of Ca(2+)-ATPase and phosphorylase A(P-A) of liver in rats was determined to study the effect of acrylonitrile (ACN) on calcium homeostasis and to clarify the toxicological mechanism of ACN. Adult male Sprague-Dawley rats were administered ACN daily per os for 42 days, at the dosage of 0, 10, 30 and 50 mg.kg-1. The activities of Ca(2+)-ATPase and phosphorylase A was determined at 14, 28, 42 days after ACN administration. The results indicated that ACN could significantly inhibit the activity of Ca(2+)-ATPase and increase the activity of P-A (P < 0.01), especially in the high dose group and exposed for 42 days, and there was significantly dose- and time-respond relationship (P < 0.01). ACN contamination could result in the disharmony of calcium homeostasis of liver in rats.

Acrylonitrile↗
PubMed1999

[Schistosoma japonicum: protective immunity induced in mice immunized with SjGST-Sj32].

Schistosoma japonicum glutathione-s-transferase (SjGST), Schistosoma japonicum 32,000 protein (Sj32) and SjGST - Sj32 were expressed and purified to study on protective immunity against Schistosoma japonicum. The result showed that compared with the adjuvant control group, the worm burden was decreased significantly in mice immunized with recombinant SjGST - Sj32, Sj32 and Sj32 + SjGST (P < 0.05). At the same time, the liver egg per gram(EPG) was decreased in mice immunized with SjGST - Sj32, Sj32, Sj32 + SjGST and SjGST. It is concluded that Sj32 significantly induces both protective immunity and anti-fecundity immunity, but SjGST only has an anti-fecundity effect.

Animals↗
PubMed1999

[Clinical analysis of 12 cases of long bone ossifying fibromas].

Twelve cases of ossifying fibroma of long bones, the lesions in 11 cases were resected with their sclerotic margin. Inactivation of the tumor cells within bone cavity was applied 50% Zinc Chloride, and autogenous bone grafts were applied with or without a few man-made bones. One case was treated by en bloc resection of the lesion. Follow-up for 3-14 years revealed that 2 cases recurred, and secondary operation was performed. After that follow-up for 5-14 years revealed no-recurrence. Recurrence rate of these cases was low and the effect of treatment was satisfactory. This kind of lesion showed the features of its aggressive growth and proneness to recur after operation. The authors consider that resection of the lesion including its sclerotic margin was effective to prevent recurrence.

Adolescent↗
PubMed1999

[Apoptosis of chondrocytes in experimental osteoarthritis].

The right knees of rabbits were immobilized in full extension for up to eight weeks using plaster cast. Specimens of the articular cartilage obtained from tibial plateau were studied by histopathologic and TDT-mediated fluorescein-dUTP nick-end labelling(TUNEL) techniques. The results showed that TUNEL-positive chondrocytes with apoptosis specific morphology were detected on superficial and middle layer of the articular cartilage from one to two weeks after immobilization, and these changes progressed until 4 weeks after immobilization. Six weeks after immobilization, TUNEL-positive chondrocytes were seen through the entire thickness of the articular cartilage. Our findings indicate that apoptosis of chondrocytes could be induced by immobilization and might be responsible for articular cartilage degeneration, and which is one of the pathways involved in the pathophysiological mechanism of osteoarthritis.

Animals↗
PubMed1999

[Effects of apoptosis of synovial lining cells on pathogenesis of osteoarthropathy].

The author counted synovial lining cells(SLCs) of apoptosis in the joints of osteoarthropathy(OA, 12 cases) and control(10 cases) patients. The counts of apoptotic SLC were investigated by gel electrophoretic analysis of DNA extracted from synovium. The apoptotic index(AI) of SLCs in OA(2.04%) was obviously higher than that in controls(0.60%); electrophorectogram of DNA revealed a characteristic ladder pattern of DNA fragments at 200 bp in the OA group. We conclude that apoptosis can be found in synovial lining layer, and the AI of SLCs in the OA group is much higher than that in the control group, implying the high turn-over rate of SLCs in OA; the increasing apoptosis of SLCs may increase lesion factors retained in joints and cause the injury of articular cartilage.

Apoptosis↗
PubMed1999

[Changes in p50 and oxygen metabolism during mild hypothermia cardiopulmonary bypass].

OBJECTIVE: To study the changes of p50 and oxygen metabolism during mild hypothermia cardiopulmonary bypass, arterial blood lactate(ABL) were measured, arterial and venous blood gas were analysed in 21 patients undergoing cardiac valve replacement. RESULTS: p50, oxygen consumption(VO2), and oxygen extract rate(ERO2) were decreased during hypothermia and increased during rewarming, respectively (P < 0.05 or P < 0.01), while the change of SvO2 was adversed. PvO2 was decreased significantly during rewarming(P < 0.01), and ABL was continuously increased. CONCLUSION: Both reduction in oxygen utilization during hypothermia and relative deficiency of oxygen supply during rewarming cause tissue hypoxia.

Adult↗
PubMed1999

[Experimental studies on hyperthermic distilled water combining with saline and dextran for preventing intraperitoneal cancer recurrence due to neoplasm seeding].

In order to know the efficacy of hyperthermic distilled water combining with saline and dextran (DSD) to prevent intraperitoneal cancer from recurrence due to neoplasm seeding, we carried out the experimental studies, including an observation on inhibiting cancer cells from implantation in vivo by abdominal cavity lavation, cytotoxic test and attachment test in vitro. The results of the tests in vitro showed no viable tumor cell exposed to 45 degrees C distilled water for 20 minutes in the tissue culture wells, meanwhile, dextran-40 partially blocked attachment of B16-F10 cells to gelatinized wells. In vivo test, DSD effectively prevented neoplasm seeding of B16-F10 viable melanoma cells in C57BL/6j mouse abdominal cavity and had no obvious toxicity. The efficiency was 70%; when cisplatin was added, the efficiency was enhanced to 88.9% with slight toxicity. These data indicate that the local application of hyperthermic distilled water combined with saline and DSD may prevent intraperitoneal neoplasm from recurring.

Animals↗
PubMed1999

[Relationship between nitric oxide and oxygen free radicals in different duration of diabetes in rat kidney].

To study the relationship between nitric oxide(NO) and oxygen free radicals in rat kidney of diabetes mellitus(DM), the authors examined the changes of renal tissue NO level and nitric oxide synthase(NOS) activity, lipid peroxidation(LPO) level and superoxide dismutase(SOD), peroxidase(POD) and catalase (CAT) activities in streptozotocin(STZ)-induced diabetic rats and normal controls(NC) at the 2nd, 8th- and 16th-week. The results showed that renal-tissue NO level and NOS activity were increased at the 2nd week, but decreased at the 8th- and 16th-week; LPO levels were significantly elevated in all DM groups than those in NC groups. SOD, POD and CAT activities decreased significantly in the 8th and 16th week DM groups compared with those in NC groups. The results suggest that NO level in kidney of STZ-induced diabetic rats dynamically change with the disease course of development. This change may be related with the change of anti-oxidation in kidney which high glucose induces.

Animals↗
PubMed1999

[Purification of outer membrane proteins in Pseudomonas aeruginosa by high performance ion-exchange liquid chromatography].

Pseudomonas aeruginosa PAO1 was used in this study. Isolation of outer membrane was accomplished by treating the cell envelope with EDTA and lysozyme, followed by centrifugation. The outer membrane (10 mg of protein) was mixed with 34 mmol/L octyl beta-glucoside-5 mmol/L EDTA-10 mmol/L Tris-HCl (pH 8.0) and subjected to supersonic oscillation for 2 min. The centrifuged supernatant (100 kgf for 30 min at 20 degrees C) was applied onto a DEAE ion-exchange high performance liquid chromatographic column (TSK gel-DEAE-5PW column, 0.75 cm x 7.5 cm i.d.) that was equilibrated with a solution of 10 mmol/L Tris-HCl buffer (pH 8.0) containing 2.5 mmol/L beta-C12E8 and 1 mmol/L EDTA. The column was washed with the same solution and eluted with a linear gradient of 0-0.5 mol/L NaCl in the same solution and fractions A, B, C were collected. Proteins in these fractions were analyzed by SDS-polyacrylamide gel electrophoresis and quantified by the method of Lowry et al. Protein E(Mr 43,000), G(Mr 25,000) and H (Mr 19,000) flowed through the column without adsorption in fraction A. Protein C(Mr 70,000), D(Mr 46,000) and a small amount of F (Mr 34,000) were eluted in fraction B. Fraction A was concentrated with ultrafiltration and applied again onto a DEAE ion-exchange HPLC column equilibrated with 10 mmol/L Tris-HCl buffer, pH 8.0, containing 34 mmol/L beta-C12E8 and 1 mmol/L MgCl2. Fraction B was subjected to DEAE ion-exchange HPLC column in the presence of EDTA. This fraction was then applied onto a DEAE ion-exchange HPLC column equilibrated with 10 mmol/L Tris-HCl buffer, pH 8.0, containing 34 mmol/L octylglucoside and 1 mmol/L EDTA. By these procedures protein C, D and E were purified to apparent homogeneity as judged by SDS-PAGE. In this work, we purified the outer membrane proteins of Pseudomonas aeruginosa, and used a new technique selectively solubilizing the cytoplasmic membrane with sodium lauryl sarcosinate for isolating the outer membrane proteins of Pseudomonas aeruginosa because of its relative simplicity.

Adhesins, Bacterial↗