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Biomedical subjects

J Zheng

Publications and source records attributed to J Zheng.

At least 235 records · Page 13Linked to original sources
PubMed1999

[Tan Yunxian, a woman physician of Ming dynasty, and her Nu yi za yan (Random talks of a woman physician)].

Inherited from the heritage of her family, Tan Yunxian read of medical works day and night. After practicing, it was said that "she always get wonderful therapeutic effects in treating those females who refuse to see a male physician". As one of the few women physicians in history, she left down Nu yi za yan which altogether 31 case reports are included, dealing with habitual abortion, menstrual disorders, postpartum diseases, and abdominal lumps, with records on moxibustion therapy applied skillfully. From a clinical view point, though only a little number of case records are included, all the treatment are successful with rich experience worth while for reference to later ages.

China↗
PubMed1999

[Clinical and experimental study on the treatment of myelodysplastic syndromes with fuzheng quxie tablet].

OBJECTIVE: To study the mechanism of Fuzheng Quxie Tablet (FZQXT) in treating myelodysplastic syndromes (MDS). METHODS: Thirty-three cases of MDS were treated with FZQXT, changes of peripheral blood cells, morphology of bone marrow smear, bone marrow pathology and immunology were monitored before and after treatment. The effects of FZQXT on hematopoietic stem cells of mice were also observed. RESULTS: The remission rate and total effective rate were 24.24% and 72.72% respectively. FZQXT had obvious effect in promoting the proliferation of colony forming unit-erythrocytes (CFU-E). CONCLUSION: The beneficial effect of FZQXT in treating refractory anemia might be reached through promoting the proliferation of erythrocytic hematopoietic stem cells.

Adolescent↗
PubMed1999

[Clinical observation on treatment of endometriosis by tonifying qi and promoting blood circulation to remove stasis and purgation principle].

OBJECTIVE: To explore the therapeutic mechanism of endometriosis by the treatment of Yiqi Huoxue Huayu Tongfu Principle (YQHXHYTFP, tonifying Qi and promoting blood circulation to remove stasis and purgation therapy) with Neiyi 1+ pill. METHODS: Forty-five cases with endometriosis were divided randomly into TCM group (30 cases, treated with Neiyi 1+ pill) and the control group (15 cases, treated with tamoxifen). The activity of natural killer cell of peripheral blood, T lymphocyte subsets, secreting interleukin-II and the EmAb were determined. RESULTS: Endometriosis is associated with immunologic disturbance. After treatment, the activity of NK cells and the count of T-suppressor cells were significantly increased (P < 0.01), the count of T-helper cells, the secretion of interleukin-II and TH/TS ratio were significantly reduced (P < 0.01). Over 50% of the cases whose EmAb was positive eventually turned to negative. The effective rate of this therapy was 90%. There was no significant difference between TCM group and the control group. CONCLUSION: YQHXHYTF therapy could modulate the immunologic disturbance in women with endometriosis, improve the clinical symptoms and signs obviously. It is a safe and effective therapy.

Adjuvants, Immunologic↗
PubMed1999

[Apoptosis increased by bcl-2 antisense ODN in MCF-7 cell line].

OBJECTIVE: To study the effects of bcl-2 antisense oligonucleotides (ODN) on the expression of bcl-2 and apoptosis in MCF-7 cell line. METHODS: Artificially synthesized bcl-2 specific antisense OND was introduced into MCF-7 cells by gene pulser electroprotocol. Fluorescence microscope, electron microscope, immunocytochemical staining assay, and FCM were used to detect the effects on cell apoptosis, expressional level of bcl-2 protein and cell cycle after transfecting bcl-2 antisense ODN. RESULTS: The number of apoptotic cells was increased and expression of bcl-2 protein was declined (P < 0.05). FCM showed that the number of G(0)-G(1) phase cells was decreased and the number of G(2)-M, S phase cells were increased. CONCLUSIONS: The effective transient expression of bcl-2 antisense ODN can be obtained by gene pulser electroprotocol in MCF-7 cell, the function of bcl-2 gene was declined and the number of apoptotic cells was increased after transfecting bcl-2 antisense ODN. bcl-2 may be used as an useful target in tumor research.

Apoptosis↗
PubMed1999

[Study on therapeutic effect of carnitine on hepatic steatosis caused by total parenteral nutrition in rats].

OBJECTIVE: To study the hepatic steatosis-minimizing effects of carnitine supplemented total parenteral nutrition (TPN). METHODS: Eighteen normal Wistar rats and nineteen cirrhotic Wistar rats were randomly divided into three groups, respectively: group A (12), chow and libitum; group B (13), TPN for one week group C (12), TPN with carnitine. Liver functions were tested at the seventh day, and also, the liver was resected for histological studies and lipid content determination. RESULTS: Hepatic lipid content was significantly elevated and hepatic steatosis was noted in group B. Hepatic lipid content and hepatic lipid deposit were significantly lowered when carnitine was added to the TPN solution. CONCLUSIONS: Carnitine can minimize hepatic steatosis caused by TPN.

Animals↗
PubMed1999

[A case-control study on cerebral palsy in children].

OBJECTIVE: To identify the risk factors for cerebral palsy (CP) in children. METHODS: A population-based 1:2 matched case-control study was conducted during May to June 1997 in southern Jiangsu Province of China. RESULTS: Risk factors for CP in children could be summarized into four categories, i.e.; (1) fetal growth retardation, preterm delivery and low birth weight; (2) asphyxia at birth, intracranial hemorrhage, ischemic and hypoxic encephalopathy and hyperbilirubinemic encephalopathy; (3) delivery with vaccum suction and traumatic brain injury; and (4) other prenatal factors. CONCLUSION: The first and second categories of risk factors correlated strongly and reproducibly with CP in children. It is emphasized that antenatal care be enforced to prevent occurrence of risk factors, such as low birth weight, etc., and the newborns with asphyxia be vigorously rescued and put under close supervision. For risk factors in the third category, the key measures should be obstetric practice and health education for the parents. But, little about the knowledge of the risk factors in the fourth category has been understood, which could be associated with those in the first two categories.

Asphyxia Neonatorum↗
PubMed1999

[A case-control study on Guillain-Barre syndrome in children of North China].

OBJECTIVE: This study aimed at exploring the risk factors for Guillain-Barre syndrome (GBS). METHODS: A case-control study design was used with 51 cases of GBS, diagnosed based on their symptoms, signs and electrophysiological examinations and exclusion of poliomyelitis and other acute flaccid paralysis, and 51 controls matched on age, sex and resident village. Serum IgG antibodies specific for Campylobacter jejuni were determined for all the subjects by enzyme-linked immunosorbent assay (ELISA) with a preparation of surface antigen of C. jejuni C(1) strain isolated from the patients and prevalent in north China. Each case and control were interviewed with his/her parents or guardians by a trained interviewer using an ad hoc questionnaire, including his/her demographic information, socioeconomic status, onset of the illness, and potential risk factors in their environment and personal hygiene. Data were analyzed with SAS software release 6.04 in a microcomputer. RESULTS: GBS was associated with a few factors, such as residential areas (45 of the 51 cases living in the rural areas, accounting for 88.2% of the total), polio and hepatitis B vaccine immunization before onset of the illness (with ORs of 7.27 and 3.14, respectively), no hand washing after defecation and before meals (with an OR of 6.15) and getting cold and going to the river or lake site before onset of the illness (with ORs of 13.75 and 12.20, respectively). Infection with Campylobacter jejuni associated strongly with the illness (with an OR of 9.5, P < 0.001). Thirty-five of the 51 cases had precursor symptoms before onset of the illness (68.6%). CONCLUSION: It suggests that occurrence of GBS may correlate to infection with Campylobacter jejuni and poor personal hygiene in children.

Antibodies, Bacterial↗
PubMed1999

[A clinico-pathological study of neuroendocrine tumors in stomach].

OBJECTIVE: To study the pathological classification of gastric neuroendocrine tumors and its clinico-pathological significance. METHODS: Paraffin sections of totally 52 gastric neuroendocrine tumors including 42 carcinoid tumors were studied with immunohistochemical technique, which involved 9 endocrine markers of hormones antibodies and electronic microscopy for investigating the endocrine cells and the contiguous gastric mucosa of the neuroendocrine tumors. RESULTS: The 52 gastric neuroendocrine tumors were divided into three types: Type I, carcinoid, associated with atrophic gastritis, altogether 26 cases. Tumor extension was limited in the mucosa or submucosa, accompanying with hypergastrinemia and G cell hyperplasia. This type is consistently preceded by and associated with generalized proliferation of endocrine cells in the mucosa at fundus. Type II, carcinoid of sporadic type, totally 16 cases, not associating with hypergastrinemia, was more aggressive. Type III, Neuroendocrine carcinomas (10 cases), were highly aggressive tumors. CONCLUSION: A correct identification of different types of gastric endocrine tumors is important and implicit for the treatment and prognosis of neuroendocrine tumors.

Adult↗
PubMed1999

[Isolation and characterization of human prostate cancer cell subclones with different metastatic potential].

OBJECTIVE: To isolate and characterize cancer cell subclones with different metastatic potential from human metastatic prostate cancer cell line (PC-3M). METHODS: Using limited dilution, in vitro growth, Matrigel invasion assay, soft agar cloning and in vivo tumorigenicity and spontaneous metastasis assay in nude mice, were isolated and characterized four subclones with different metastatic potential. RESULTS: Four subclones derived from PC-3M were 1E8, 2E7, 2B6, 2B4. Each subclone exhibited a different metastatic potential when inoculated into nude mice. Among these subclones, 1E8 expressed as the highly metastatic phenotype, with 100% metastasis frequency 5 weeks after subcutaneous inoculation into nude mice, whereas 2B4 was not metastatic. In vitro 1E8 was found to be the most highly invasive cell line in Matrigel invasive assay (98 +/- 24) and had the most clones in soft agar cloning assay (265 +/- 39) while 2B4 was found to have the least invasive abilities (12 +/- 4) and the least clones (137 +/- 14). CONCLUSION: Successful establishment of these subclones with different metastatic potential may be valuable for further study on the molecular mechanisms of cancer metastasis and cloning of cancer metastasis-related genes.

Animals↗
PubMed1999

[A clinicopathologic study on eight cases of cystic and solid tumors of pancreas].

OBJECTIVE: To study the clinicopathologic characteristics, differentiation patterns and histogenesis of cystic and solid tumors of the pancreas (CSTP). METHODS: 8 cases of CSTP were studied using histologic (HE and PAS), immunohistochemical (S-P method) and electron microscopic techniques. RESULTS: All the patients were adolescent and young adult females, 14-33 years in age (mean 25.3 years) without recurrence after tumor resection. The mean diameter of tumors was 9.6 cm, all encapsulated. Histological examination showed presence of solid sheets, pseudopapillary, in all of the cases. Hemorrhage, foam cells, and cholesterol crystals were often found. Immunohistochemically, 8 cases were positive for alpha(1)-AT and lysozyme; 6 cases expressed vimentin, 2 cases expressed actin, and CgA-positive cells found in the tumor cell nests in one case. All of the cases showed PR, and 4 cases showed ER positive immunoreactivity in the majority of tumor cells, but negative for CK AE1, CK AE3, EMA, Synaptophysin, ACTH, gastrin, somatostatin, insulin, and glucogan in all the cases. Electron microscopy of 3 cases showed evidences of polymorphism in differentiation of the tumor cells, including the transitional appearance into ducts, acinus, and endocrine cells. Weibel-Palade body found in tumor cells in one out of 8 cases. CONCLUSIONS: (1) CSTP is a distinct clinicopathologic entity in young female patients with a benign clinical course. (2) CSTP develops from primitive pancreatic cells, with the potentiality of developing into ducts, acinus, and endocrine cells.

Actins↗
PubMed1999

[Chemical constituents of Pistia stratiotes L].

OBJECTIVE: To investigate the chemical constituents of Pistia stratiotes, a kind of duckweed. METHOD: Compounds were separated by chromatography on sillica gel, and their structures were identified by spectral analysis and chemical evidence. RESULT: Four compounds were obtained and identified as stigmasta-4,22-dien-3-one, stigmasterol, stigmasteryl stearate and palmitic acid. CONCLUSION: All the four compounds were isolated from this plant for the first time.

Araceae↗
PubMed1999

[A pharmacognostical study on herba Spirodelae and its adulterants].

OBJECTIVE: To Study the characteristics of Herba Spirodelae(Spirodela polyrrhiza) and its adulterants S. oligorrhiza and Lemna minor for identification. METHOD: The characteristics were compared and identified morphologically and histologically. RESULT: The morphological and histological characteristics of S. polyrrhiza, S. oligorrhiza and L. minor are different from each other. Several tables, keys and drawings are given. CONCLUSION: The main macroscopical and microscopical characteristics can be used fo differentiate S. polyrrhiza from its adultrants.

Drug Contamination↗
PubMed1999

[Isolation and identification of two flavonoids from Taraxacum mongolicum Hand.-Mazz].

OBJECTIVE: To study the chemical constituents of Taraxacum mongolicum. METHOD: Compounds were separated by column chromatography on silica gel and polyamide, and their structures were determined by spectral analysis and chemical evidence. RESULT: Two flavonoid compounds were separated and elucidated as isoquercitrin and hyperin. CONCLUSION: These two compounds were isolated from the plant for the first time.

Flavonoids↗
PubMed1999

[Determination of lignan glycosides in the roots of Gaultheria yunnanensis Rehd. by HPLC].

OBJECTIVE: To determine the contents of four lignan glycosides in the root of Gaultheria yunnanensis, namely, D1: gaultheroside A, D2:(-)-8'-methoxyisolariciresinol-2a-O-beta-D-xylopyranoside, D3:(-)-isolariciresinol-2a-O-beta-D-xylopyranside, D4:(+)-lyoniresinol-2a-O-beta-D-glucoxylopyranoside. METHOD: Rp-HPLC was used to separate and analyze the lignan glycosides in the root of the title plant. RESULT: The method for sample preparation was set up; the gradient program for the mobile phase was established; and the retention times of four lignan glycosides were determined. CONCLUSION: The method has been proved to be accurate, reproducible and of higher recovery rate.

Chromatography, High Pressure Liquid↗
PubMed1999

[Studies on human nasal cycle with acoustic rhinometry].

OBJECTIVE: The aim of this article was to study the classifications and characteristics of nasal cycle of adult and children and the influence of pathological state of nasal mucosa on nasal cycle with acoustic rhinometry. METHOD: We examined the nasal cycle of 16 healthy adults, 11 healthy children and 14 patients with bilateral nasal obstruction. Acoustic rhinometry was performed every 30 minutes (20 minutes in children) over 4-8.5 hours to determine minimum cross-sectional area of nasal cavity (MCA) and nasal cavity volume (NV) which were used as indices of nasal patency. RESULT: Nasal cycles were observed in all subjects. Classical nasal cycle was most commonly observed in healthy adults and patients with nasal diseases. In healthy children, in concert nasal cycle was the most common type. The distribution of the healthy adults and patients with nasal diseases between the cycle categories was not statistically significant different (P > 0.05), the mean periodicity of the cycle was no significantly different between these two groups (P > 0.05). The mean alteration amplitude of nasal cycle of healthy adults was significantly longer than that of patients with nasal diseases (P < 0.01). The distribution of the healthy children and the healthy adults between the cycle categories was significantly different (P < 0.01), the mean periodicity of the cycle of healthy children was distinctively shorter than that of healthy adults (P < 0.01). CONCLUSION: The above results illustrated that nasal cycle exists as central control and local modulation. In healthy children, in concert nasal cycle was most commonly observed and this may be related with the immature condition of their autonomic nervous system.

Adult↗
PubMed1999

[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection].

A rapid, isocratic high performance liquid chromatographic(HPLC) method with fluorescence detection was used for the determination of total homocysteine (Hcy) levels in human plasma. Prior to reversed-phase HPLC analysis, the plasma thiols were derivatized with ABDF(7-fluoroben30-2-oxa-1,3-diazole-4-sulfonamide), a thiol specific fluorogenic probe. A mobile phase consisted of 0.1 mol/L potassium dihydrogen phosphate (pH 2.1, containing 10% of acetonitrile) ensured base line separation of plasma Hcy. The mean recovery of this method was 97.75%. The intraday and interday coefficient of variations were 4.49% and 9.79%, respectively. The method is simple, sensitive, reproducible and very suitable for routine determination of plasma Hcy in human.

Adult↗
PubMed1999

[Prospects for antisense techniques].

Antisense technique, developed on the principle of base complementation, is a method using short strand oligonucleotide fragments complementary to target DNAs or target RANs for inhibition of gene expression. As a theoretically perfect and specific method to block the expression of gene, it has been rapidly developed over the last twenty years. This review expatiates on its techniques, its problem and possible way of problem-solving, and its recent application in medicine. Also this review discusses the trends of its further development.

Animals↗