Search PubMed⌕ Search

Biomedical subjects

J Zhao

Publications and source records attributed to J Zhao.

At least 631 records · Page 35Linked to original sources
PubMed1995

[Compositive treatment of acute deep vein thrombosis of lower extremity].

Fourteen patients with acute deep vein thrombosis of lower extremity (DVT) were treated with composite methods including thrombectomy (iliofemoral venous thrombectomy and ascending venous thrombectomy), thrombolytic and anticoagulant therapy. Follow-up for 2-27 months (average 11.5 months) showed excellent and good results in 78.6%, and the morbidity of post-thrombotic syndrome only 21.4%. The remarkable results were due to opening of occlusive vein of the lower extremities by thrombectomy, which could make thrombolytics effective and reduce the dose of thrombolytics to prevent bleeding complication. Ascending venous thrombectomy could not only remove the venous thrombi of the lower extremities but also protect the deep venous valves and reduce the morbidity of postthrombotic syndrome. Additionally, to reach a satisfied therapy, lesion in iliac vein, such as anatomical compression, stenosis, occlusion, diaphragm and so on, shall be managed as well. Otherwise, DVT shall be recurrent.

Adult↗
PubMed1995

[The role of sympathetic mechanism in the effect of electroacupuncture on immunoregulation].

1. In normal control mice (only injected SRBC) after EA the lymphocyte transformation test (LTT, P < 0.05) and interlukin-2 (IL-2) activity (P < 0.05) were significantly increased, but the serum lysozyme (IZM, P < 0.01) was apparantly decreased. 2. After injection of 6-OHDA in mice, the spleen weight, spleen index, thymus weight, thymusindex, spleen lymphocyte population and serum IZM, igG contents were markedly decreased, whereas LTT, IL-2 and plaque forming cell (PFC) were umchanged, In contrast, the thymus lymphocyte population was markedly increased. 3. After EA of mice axotomized sympathetic nerve with 6-OHDA the above mentioned immune parameters deceased by 6-OHDA could be returned to normal or nearing normal levels. However, the unchanged LTT, IL-2 and the increased thymus lymphocyte population could be enhanced further, especially IL-2. These results suggest that peripheral sympathetic nerve may be participation in the EA-mediating immunological responses.

Animals↗
PubMed1995

Brain activity of a rat reflects apparently the stimulation of acupuncture. A radioautography using 2-deoxyglucose.

To confirm a relationship between the central nervous system and the acupuncture, the response was examined in the rat brain using radioautography with tritiated 2-deoxyglucose, after stimulation of some acupoints. Eight groups, of a total of 27 rats were submitted to acupuncture at 6 different acupoints (Zusanli, Renzhong, Tianshu, Shenmen, Neiguan, Yongquan) and control, with or without electric pulses or with anesthesia respectively, prior to the injection with isotopic deoxyglucose. A hundred twenty cryosections were cut from a freshly frozen brain and exposed on single-coated X-ray films. The results showed that the acupuncture on the four acupoints of Zusanli, Tianshu, Shenmen and Neiguan enhanced the activity of the neocortex, the limbic cortex and the thalamic nuclei in comparison with the control group. The acupoints on Renzhong and Yongquan depressed the activity on the thalamic nuclei and the midbrain. The anesthesia with pentobarbital concealed the most activity on the whole area of the brain, which hardly responded to any stimulation of acupuncture. These results lead to the suggestion that the brain may reflect the signals by the stimulation of acupuncture and change the activity depending upon each acupoint.

Acupuncture Therapy↗
PubMed1995

Rapid diagnosis and treatment of chlamydial conjunctivitis.

During December 1989 to December 1992, conjunctival specimens from 63 patients with severe trachoma and 30 with acute follicular conjunctivitis at the eye clinic of Tong Ren Hospital in Beijing, were tested by using direct immunofluorescent technique and amplified enzyme-linked immunoassay (Micro Trak and IDEIA) for chlamydial antigenicity. Trachoma accounted for 97.6% while acute follicular conjunctivitis accounted for 2.4% of the positive cases. Micro Trak and IDEIA tests proved advantageous over the culture method for the detection of Chlamydia trachomatis. In our study, Ofloxacin eye ointment has been proved to be an alternative drug for severe trachoma with better curative effectiveness.

Adolescent↗
PubMed1995

Experiment study on the effect of Sheng-Mai injection on the contractivity of diaphragm and its mechanism.

In this study the effect of Sheng-Mai Injection i.e. Red Ginseng-Ophiopogon Root Injection (one kind of traditional Chinese medicines) on the contractivity of diaphragm was observed. The results confirmed that Sheng-Mai Injection increased Pdi of the fatigued diaphragm in rabbits and reduced the time needed for the recovery of Pdi of fatigued diaphragm to the normal value. These results suggest that Sheng-Mai Injection can increase the contractive force and promote the recovery of the fatigued diaphragm. The effect of Sheng-Mai Injection on the contractivity of the isolated diaphragmatic bundle of rats was also observed and the results confirmed that Sheng-Mai Injection increased the diaphragmatic contractive force directly. This effect of increasing the contractive force of diaphragm was attenuated by adding calcium channel blocker isoptin and disappeared when there was no calcium in the extracellular fluid. It is deduced, therefore, that the mechanism of the effect of Sheng-mai Injection is related to the increased influx of calcium from extracellular fluid into the cells.

Animals↗
PubMed1995

[Isolation and identification of enteric adenovirus in China].

Enteric adenovirus type 40/41 is considered to be the second major cause of gastroenteritis in young children. In this study fecal specimens 86-123 from diarrhea patients were isolated and examined in Grahm 293 cells. This induced cytopathic effect (CPE) at this cells. Viral particles were also found in fecal specimens and by electron microscopy. Examination of the isolate with Cambridge Biotech Adenoclone-Type 40/41 test kit indicated that it contained EAd. On the basis of the above studies, one strain of EAd was the first isolated virus in China.

Adenoviruses, Human↗
PubMed1995

[Postoperative residual intracranial aneurysms].

From January 1991 to 1994, 99 aneurysms in 91 patients were clipped in the Neurosurgy Department of Beijing Tiantan Hospital. Six residual aneurysms were found by postoperative angiography. We analyzed these patients and concluded that the occurrence of residual aneurysms depends mainly on the location, size, type, neck width of the aneurysms, and their rupture during operation. According to this, we give opinions on how to prevent residual aneurysms and how to deal with them.

Adult↗
PubMed1995

[Multimodality therapy of late stage lung cancer].

A prospective randomized study of 80 cases of stage III lung cancer treated with different multimodality therapy was carried out. Life table and Kaplan-Meir curve were employed to calculate survival rate. Log rank-multivariate analysis and time t test were used to evaluate statistical values of the 80 cases, 40 SCLC were randomly treated with either chemotherapy (CT)-surgery-CT or CT-radiotherapy (RT)-CT. The year survival rates were better in the group treated with CT-surgery-CT, a statistical difference was observed in 2yr survival rate (P < 0.05). Thus, surgical resection for SCLC was better than RT after CT. The remaining 40 cases of NSCLC were randomly treated with either CT-RT-CT or RT-CT. Multivariate analysis showed a better statistical meaning in the 20 cases treated with CT-RT-CT than the other group, their 1, 2 year survival rates were 27%, 40% and 22%, 15%, respectively. Thus preradiative CT was beneficial for survival.

Adult↗
PubMed1995

[Investigation of the application of electrical impedance principle for the diagnosis of diaphragm fatigue].

We designed and performed the chest and abdomen impedance respirograph (IRG), which included the one dimentional IRG and the two dimentional IRG, by applying the principle of bioelectrical impedance. Using IRG, we measured the non-synchronized chest and abdomen respiratory motions occurring in the diaphragmatic fatigue. The results showed that all 203 normal control subjects showed synchronized pattern of chest and abdomen respiratory motions. In 189 COPD patients 117 (61.9%) showed non-synchronized respiratory motions which could be further divided into three types: type I showed complete contradirectional movements of respiration, M > 24% and alpha angle > 120 degrees; type II showed staggered peak of the chest and abdomen motion curves, 13% < M < 24%, 50 degrees < alpha angle < 120 degrees; type III showed double peaks in the one dimentional IRG and 8-shaped double circles in the two dimentional IRG, M < 13%, alpha angle > 40 degrees. When compared with Pdi and diaphragm myoelectricity frequency spectrum the rates of accordance were 81.8% and 90%, respectivity, suggesting that IRG could be reliably used to diagnose diaphragmatic fatigue. This technique is simple, easy, cheap and non-invasive. It is, therefore, worth to be widely recommanded for clinical investigation.

Aged↗
PubMed1995

[Accumulation sites of kanamycin in cochlear basal membrane cells].

The dissection technique of cochlear basal membrane, the fractional technique of tissular homogenization, and the radiometry technique were used to study the toxicity of kanamycin on the hair cells. It was found that after the cochlea was perfused with a 0.25% perilymph solution of kanamycin, there were 3.14 fg KM in a nucleus, 7.61 fg KM in a mitochondria and 21.16 fg KM in a lysosome. It showed that after entering the cell, KM mainly accumulated with in the mitochondria. Simultaneously the lysosome also phagocytized a lot of kanamycin. The germicidal mechanism of kanamycin is to inhibit the synthesis of protein in bacteria and the mitochondria has the same way of protein synthesis, the mitochondria can combine with kanamycin specifically; the drug in lysosome may be related to its phagocytic function.

Animals↗
PubMed1994

Identity of a peptide domain of human C9 that is bound by the cell-surface complement inhibitor, CD59.

The CD59 antigen is a plasma membrane glycoprotein that serves as an inhibitor of the C5b-9 complex of complement. This inhibitory activity appears related to the capacity of CD59 to bind with high affinity to sites that are nascently exposed in the alpha-chain subunit of human C8, as well as within the C9b domain (amino acid residues 245-538) of human C9, during assembly of the C5b-9 complex on the target membrane (Ninomiya, H., and Sims, P. J. (1992) J. Biol. Chem. 267, 13675-13680). The CD59 binding site in C9 was first investigated by N-terminal sequencing of CD59-binding peptides generated by limited digest of the isolated C9b domain. These experiments revealed a 17-kDa fragment (starting at C9 residue Thr-320) that retained affinity for CD59, suggesting the possibility for localizing the CD59 binding site by mapping with small C9-derived peptides. Peptides spanning the entire C9b sequence were expressed in Escherichia coli and then probed with CD59. CD59 bound specifically to all peptides starting N-terminal to C9 residue 359 with C termini extending beyond residue 411. Little to no CD59 binding was observed for various C9-derived peptides that started C-terminal to residue 359 or that were truncated N-terminal to residue 411. Affinity-purified antibody against C9 residues 320-411 inhibited CD59 binding to C9 by > 50% and completely inhibited its binding to the isolated C9b domain. Little to no specific binding of CD59 was detected for peptides restricted to the putative hinge domain within C9b (residues 245-271). These results indicate that a CD59 binding site is located between residues 320 and 411 of the C9 polypeptide and suggest that the affinity of this site is principally determined by residues 359-411.

Amino Acid Sequence↗
PubMed1994

1H and 15N NMR assignments of PsaE, a photosystem I subunit from the cyanobacterium Synechococcus sp. strain PCC 7002.

PsaE is a highly conserved, water-soluble protein of the photosystem I reaction center complexes of cyanobacteria, algae, and green plants. Along with the PsaC and PsaD proteins, the PsaE protein binds to the stromal surface of photosystem I and is required for cyclic electron transport in Synechococcus sp. strain PCC 7002 [Yu, L., Zhao, J., Mühlenhoff, U., Bryant, D.A., & Golbeck, J.H. (1993) Plant Physiol. 103, 171-180]. The psaE gene from this cyanobacterium encodes a mature protein of 69 amino acid residues and has recently been overexpressed in Escherichia coli [Zhao, J., Snyder, W.B., Mühlenhoff, U., Rhiel, E., Warren, P. V., Golbeck, J. H., & Bryant, D. A. (1993) Mol. Microbiol. 9, 183-194]. By using both unlabeled and uniformly 15N-labeled protein in a series of two- and three-dimensional NMR experiments, complete 1H and 15N amide resonance assignments were made. The major secondary structural element of PsaE is a five-stranded antiparallel beta-sheet. The five strands extend as follows: beta A, residues 7-10; beta B, residues 21-26; beta C, residues 36-39; beta D, residues 57-60; and beta E, residues 65-68. The topology is represented by (+1, +1, +1, -4x); it brings the first and last strands, and consequently the N- and C-termini, together. The protein has an extensive hydrophobic core organized around a conserved phenylalanine residue (Phe-40); another of its distinctive features is a segment extending from residue 42 to residue 56 devoid of dipolar contacts with the beta-sheet. The pK1/2 of the sole histidine residue (His-63) was determined to be 5.4.

Amino Acid Sequence↗
PubMed1994

Three-dimensional solution structure of PsaE from the cyanobacterium Synechococcus sp. strain PCC 7002, a photosystem I protein that shows structural homology with SH3 domains.

PsaE is a 69 amino acid polypeptide from photosystem I present on the stromal side of the thylakoid membrane. The three-dimensional solution structure of this protein from the cyanobacterium Synechococcus sp. strain PCC 7002 was determined at pH 5.8 and room temperature using over 900 experimental restraints derived from two- and three-dimensional NMR experiments. The structure is comprised of a well-defined five-stranded beta-sheet with (+1, +1, +1, -4 alpha) topology. There is no helical region except for a single turn of 3(10) helix between the beta D and beta E strands. PsaE also exhibits a large unrestrained loop spanning residues 42-56. A comparison to known protein structures revealed similarity with the Src homology 3 (SH3) domain, a membrane-associated protein involved in signal transduction in eukaryotes. The match is remarkable as 47 of the alpha-carbons of PsaE can be superimposed onto those of the SH3 domain from chicken brain alpha-spectrin with a root-mean-square deviation of 2.3 A. Although the amino acid sequences have low identity and the loops are different in both proteins, the topology of the beta-sheet and the 3(10) turn is conserved. SH3 domains from other sources show a similar structural homology. The structure of PsaE was used to suggest approaches for elucidating its roles within photosystem I.

Amino Acid Sequence↗
PubMed1994

Experimental studies of the inhibitory effects of green tea catechin on mice large intestinal cancers induced by 1,2-dimethylhydrazine.

Three hundred Kunming mice were randomly divided into six groups (half males and half females in each group). Group 1 was the positive control group, Groups 2, 3, 4 and 5 were experimental groups and Group 6 was used as the solvent control group. Mice in Groups 1-4 were injected with 1,2-dimethylhydrazine (1,2-DMH) (20 mg/kg body wt.) solution subcutaneously once a week from the 2nd week to the 20th week. From the 1st week to the 23rd week, mice in Groups 2, 3 and 4 were given catechin (1 mg/mouse), catechin (2 mg/mouse) and EGCG (2 mg/mouse), respectively, five times a week. Mice in Group 5 received only catechin (3 mg/mouse) five times a week from the 1st to the 23rd week. Mice in Group 6 were injected with an equal volume of 1 mmol EDTA solution subcutaneously once a week from the 2nd to the 20th week. At the end of the 27th week, all the mice were killed by cervical dislocation (Zhu, Q.H. and Zhu, Q.F. (1991) Laboratory Animal Science, 1st edition. The Junior Educational Publisher, Guangdong). Pathological examinations indicated that the incidence of large intestinal cancers occurring in Group 1 was 80%, significantly higher than that in Groups 2, 3 and 4 (p < 0.001). No tumors were found in Groups 5 and 6. This might suggest that green tea has preventive effects on large intestinal cancer induction in spite of the different doses of catechin. Immunohistochemistry studies showed that green tea catechins could enhance the activity of superoxide dismutase (SOD) in tissues.

1,2-Dimethylhydrazine↗
PubMed1994

Differential expression and ligand-induced modulation of the human interleukin-6 receptor on interleukin-6-responsive cells.

The human interleukin-6 receptor (IL-6R) was differentially expressed on IL-6-dependent (U266 and SKO-007) and -independent (RPMI8226) myeloma cells as well as melanoma cells (A375-C6) that are growth-inhibited by IL-6. U266 and SKO-007 cells expressed four distinct IL-6R complexes (molecular masses of 100, 120, 145, and 165 kDa) as revealed by affinity cross-linking of iodinated IL-6. RPMI8226 and A375-C6 cells primarily expressed the 165-kDa complex relative to the others. Immunoprecipitation and antibody competition studies showed that the 100- and 120-kDa complexes contained the gp80 subunit, whereas the 145- and 165-kDa complexes contained the gp130 subunit of the IL-6R. Assaying solubilized U266 plasma membrane proteins by affinity cross-linking or ligand blotting revealed that only gp80 bound IL-6 specifically. Induction of an IL-6 response was associated with ligand-induced down-regulation of gp130 and was inhibited by neutralizing anti-IL-6 antibodies. Furthermore, the relative ratios of gp80 to gp130 determined the binding kinetics of the IL-6R, yielding high- and low-affinity binding sites by Scatchard plots. Our data imply that distinct IL-6 bioactivities are based upon the differential expression and regulation by IL-6 of its ligand-binding (gp80) and signal-transducing (gp130) receptor subunits.

Binding, Competitive↗
PubMed1994

Risk factors for esotropia and exotropia.

OBJECTIVE: To identify risk factors associated with the two major types of strabismus--esotropia and exotropia--in a cohort of children followed up from gestation to age 7 years. DESIGN: Pregnant women were enrolled in the Collaborative Project of the National Institute of Neurological Disorders and Stroke, Bethesda, Md, from 1959 to 1965 at 12 university centers. This large multidisciplinary study was designed to evaluate the developmental consequences of complications during pregnancy and the perinatal period. Data on maternal, socioeconomic, perinatal, and neonatal characteristics were collected from 39,227 children and their mothers by medical examination and interview. Examinations of the children were performed at birth, 4 months, 8 months, 1 year, and 7 years. OUTCOME MEASURES: The evaluation of the presence of strabismus was performed during follow-up examinations and confirmed at the 7-year follow-up visit. Potential risk factors for strabismus were evaluated from the maternal, socioeconomic, perinatal, and neonatal characteristics. RESULTS: Esotropia developed in 1187 children (3.0%), and exotropia developed in 490 children (1.2%). Esotropia was more common in whites (3.9% in whites vs 2.2% in blacks, P < .0001). The occurrence of exotropia was similar in the two races (1.2% in whites and 1.3% in blacks). Results of multivariable logistic regression models showed that the risk of strabismus increased with low birth weight (P < .0001). For infants weighing 1500 g at birth compared with those weighing 4000 g at birth, the odd ratios were 3.26 (95% confidence interval, 2.50 to 4.25) for esotropia and 4.01 (95% confidence interval, 2.77 to 5.80) for exotropia. Maternal cigarette smoking during pregnancy also increased the risk of each type of strabismus (P < .0001). For offspring of mothers who smoked more than two packs of cigarettes per day compared with those whose mothers did not smoke, the odds ratios were 1.83 (95% confidence interval, 1.51 to 2.22) for esotropia and 2.32 (95% confidence interval, 1.72 to 3.13) for exotropia. Maternal age was also a significant risk factor for esotropia (P = .0005). The risk of esotropia increased with increasing age until age 34 years. In particular, the odds ratio for mothers aged 30 to 34 years relative to that for mothers aged 20 to 24 years was 1.43 (95% confidence interval, 1.19 to 1.70). CONCLUSIONS: Esotropia was more common in whites than in blacks. The occurrence of exotropia was similar in the two races. Maternal cigarette smoking during pregnancy and low birth weight were independent and important risk factors for both esotropia and exotropia. There was an increased risk of esotropia with increasing maternal age.

Adult↗
PubMed1994

Expression, purification, and characterization of thymidylate synthase from Lactococcus lactis.

The thymidylate synthase (TS) gene from Lactococcus lactis has been highly expressed in Escherichia coli. The TS protein was purified by sequential chromatography on Q-Sepharose and phenyl-Sepharose. Six grams of cell pellet yielded 140 mg of homogeneous TS. TS is a highly conserved enzyme, and several of the conserved amino acid residues that have been implicated in catalytic function are altered in L. lactis TS. By use of a 3-dimensional homology model, we have predicted covariant changes that might compensate for these differences. With the large amounts of L. lactis TS now available, studies can be pursued to understand the structure-function relationships of this enzyme compared to other TSs and to confirm the presumed roles of the compensatory changes predicted in the homology model.

Amino Acid Sequence↗