Biomedical subjects

J Zhao

Publications and source records attributed to J Zhao.

At least 289 records · Page 16Linked to original sources

PubMed2000

[Application of intermittent-feeding of growth-limiting nutrients in suspension culture of insect cells(Sf21)].

On the basis of the growth and metabolism behavior inherent in suspended Sf21 insect cells, the intermittent feeding of growth-limiting nutrients(glucose and protein hydrolysates) was employed in the regulation of cellular growth during the cultivation by using the residual glucose concentration as a reference point. It was shown that as compared with the batch cultivation, the intermittent-feeding of growth-limiting nutrients effectively prolonged the growth and stationary phase for Sf21 insect cells grown in two representative culture medium(TC-100 and IPL-41). The maximum cell density was increased from 3.0 x 10(6) cells/ml to 6.5 x 10(6) cells/mL in TC-100 medium, and in IPL-41 medium, the maximum cell density was increased from 7.05 x 10(6) cells/mL to 9.0 x 10(6) cells/mL. As the defined nutrient solution was used for feeding in lieu of the complicated and expensive base medium, the technique would find prospects in large scale high-density cultivation of insect cells.

Animals↗

PubMed2000

[Chromosome localization of the dentinogenesis imperfecta type II locus].

OBJECTIVE: To scrutinize the linkage between dentinogenesis imperfecta type II and chromosome 4q21 in a Tianjin-Tanggu family. METHODS: Blood samples were collected from 13 members of the family. DNA was analyzed with 4 short tandem repeat polymorphisms markers UGATA62A11, DSP(P), SPP1 and D4S1563 Y using fluorescence-based PCR. The linkage between four markers on chromosome 4q21 and dentinogenesis imperfecta type II was tested by Lod score analysis. RESULTS: GATA62A11 and DSP(P) suggested linkage and yielded a Lod score of 1.63 at theta =0, and 1.68 at theta =0 by means of the MLINK software, respectively. Genotype and haplotype were acquired. CONCLUSION: The disease gene of the dentinogenesis imperfecta type II family is located on chromosome 4q. The result will be helpful for the further identification of the dentinogenesis imperfecta type II gene.

Chromosome Mapping↗

PubMed2000

[The study on hereditary polymorphism of thiopurine S-methyltransferasein Chinese Han population of Shanghai area].

OBJECTIVE: To gain an insight into the hereditary polymorphism of thiopurine methyltransferase(TPMT) activity in Chinese Han population of Shanghai. METHODS: The present authors measured the erythrocyte TPMT activity in 320 healthy Chinese volunteers and 51 children with acute lymphoblastic leukemia(ALL) by means of radiochemical assay. RESULTS: The TPMT activity levels ranged from 4. 32 to 32.33 U/ml pRBCs, with a mean value of (16.64+/-4.50) U/ml pRBCs, male (16.78+/-4.96) U/ml pRBCs, female (16.52+/-4.44) U/ml pRBCs. Eight point one percent of the sample had low activity. The TPMT activity levels for subjects who were <12 years, 13-18 years, 19-45 years and >45 years old were (16.52+/-4.31) U/ml pRBCs, (16. 71+/-4.24) U/ml pRBCs, (16.28+/-5.21) U/ml pRBCs and (17.11+/-3.98) U/ml pRBCs, respectively; the TPMT activity levels for healthy volunteers and patients with ALL were (16.65+/-4.72) U/ml pRBCs and (16.52+/-4.47) U/ml pRBCs, respectively. CONCLUSION: There were no differences of TPMT activity in gender, age, and between healthy volunteers and patients with ALL.

Adolescent↗

PubMed2000

[The expression of transforming growth factor-beta receptor I and its mRNA in liver tissues of chronic hepatitis B and the clinical significance].

OBJECTIVE: To study the expression of transforming growth factor-beta receptor I (TGF-beta RI) and its mRNA in liver tissues of chronic hepatitis B (CHB) and the relationship between its expression and the pathological injury of hepatic tissues. METHODS: Forty-eight liver specimens from patients with CHB were observed by techniques of immunohistochemistry and in situ nucleic acid hybridization. RESULTS: TGF-beta RI was expressed in the hepatocyte, sinusoid endothelial cell and bile duct epithelial cell, with predominance in the hepatocyte and distribution in small granule or cell membrane. Increased expressing of TGF-beta RI was parallel to the degree of inflammatory activity and fibrosis (r=0.7095, P<0.05; r=0.6915, P<0.05; respectively). The number and the distribution of TGF-beta RI mRNA in the positive cells were coincident to those of its protein. The expression of TGF-beta RI was closely correlated with Tbil (r= 0.3396, P<0.05), PA (r= -0.3430, P<0.05) and ALB (r= -0.4173, P<0.01). CONCLUSION: The TGF-beta RI may involve in the pathological injury of hepatic tissues of CHB.

Activin Receptors, Type I↗

PubMed2000

Biomechanical and morphometric properties of the arterial wall referenced to the zero-stress state in experimental diabetes.

Morphometric and passive biomechanical properties were studied in isolated segments of the thoracic and abdominal aorta, left common carotid artery, left femoral artery and the left pulmonary artery in 20 non-diabetic and 28 streptozotocin (STZ)-induced diabetic rats. The diabetic and non-diabetic rats were divided into groups living 1, 4, 8, and 12 weeks after the induction of diabetes (n = 7 for each diabetic group) or sham injection (n = 5 for each group). The mechanical test was performed as a distension experiment where the proximal end of the arterial segment was connected via a tube to the container used for applying pressures to the segment and the distal end was left free. The vessel diameter and length were obtained from digitized images of the arterial segments at pre-selected pressures and at no-load and zero-stress states. Circumferential and longitudinal stresses (force per area) and strains (deformation) were computed from the length, diameter and pressure data and from the zero-stress state data. The zero-stress state was obtained by cutting vessel rings radially causing the rings to open up into a sector. Diabetes was associated with pronounced morphometric changes, e.g., wall thickness. With respect to the biomechanical data, the opening angle increased and reached a plateau in 4 weeks after which it decreased again (p < 0.05). The opening angle was smallest in the thoracic aorta and largest in the pulmonary artery. Furthermore, it was found that the circumferential stiffness of the arteries studied increased with the duration of diabetes. In the longitudinal direction significant differences were found 8 weeks after injection of STZ in all arteries except the pulmonary artery. In the 12 weeks group, the femoral artery was stiffest in the circumferential direction whereas the thoracic aorta was stiffest in the longitudinal direction. The accumulated serum glucose level correlated with the arterial wall thickness and elastic modulus (correlation coefficient between 0.56 and 0.81).

Animals↗

PubMed2000

The research and development of noncontact 3-D laser dental model measuring and analyzing system.

OBJECTIVE: To research and develop a new laser scanning 3-dimensional digitization system for collecting, showing, and measuring the data of dental casts. MATERIAL AND METHODS: According to the 3-dimensional measuring method, by using 2 pulsate motors to make the synthetic movement of the dental cast, a special semiconductor laser, and 2 special line array CCD, the 3-D data could be found anywhere on the surface of the dental cast. The program, developed with Visual C++ language under the Windows (Windows tm) system, can control the scanner, rebuild the 3-D graphics and measure the data of the dental cast. RESULTS: The system offers 70 x 70 x 70 mm measurement scope, 0.01 mm resoling power, < 0.1 mm mean deviation, and 25 mins for single cast scanning. CONCLUSION: The advantages of this system are its precision, simplicity, high efficiency, and wide range of measurements with complete direct view. This system also supplied new information that cannot be found with traditional measurement methods.

Computer Graphics↗

PubMed2000

[Study on relationship between the apoptosis of hepatocytes and liver fibrosis of chronic viral hepatitis].

OBJECTIVE: To study the relationship between apoptosis of hepatocytes and liver fibrosis of chronic viral hepatitis (CH). METHODS: DNA fragmentation, one of the characteristic features of apoptosis, was detected by in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and the expression of Fas, transforming growth factor beta 1 (TGF-beta 1) and Pre-collagen III peptide (P III P) in liver tissues were detected by immunohistochemistry. Soluble Fas (sFas) and TGF-beta 1 in serum were detected by ELISA. RESULTS: The damage of DNA in hepatocytes of CH correlated closely with the expression of Fas and TGF-beta 1 in liver tissue and serum (r = 0.6129, 0.5368, 0.5564, 0.5996; P <0.05-0.01). In severe type CH and liver cirrhosis, the degree of liver fibrosis and the expression of P III P and TGF-beta 1 were higher than that in mild type and moderate type CH. CONCLUSIONS: The sFas in serum may be one of the factors associated with the apoptosis. The apoptosis of hepatocytes correlated with liver fibrosis of CH and TGF-V beta 1 may play an important role between apoptosis and liver fibrosis.

Adolescent↗

PubMed2000

[Viral etiology study of acute conjunctivitis].

OBJECTIVE: To study the viral etiology of acute conjunctivitis broke out in Shenzhen in 1997. METHODS: Isolated the virus from eye and pharynx swabs of acute conjunctivitis in acute stage, and detected virus nucleic acid by polymerase chain reaction (PCR). DNA of viral strains were subjected to restriction enzyme analysis using several endonucleases. Also, neutralizing antibody of paired sera were detected. RESULTS: The positive virus isolation rates of 14 pharynx and eye swabs were 71.43% and 64.29%, respectively; and that of the virus DNA by PCR were 78.57% and 71.43%, respectively. All of the strains isolated were adenovirus type 3. Three paired viral strains chosen and subjected to restriction enzyme analysis proved belonging to Ad3a2 gene type. The neutralization test of paired sera of 24 cases showed that the antibody titers had 4 fold rising or above. Neutralization test of isolated viruses with paired sera of itself was carried out, the level of neutralizing antibody in convalescent stage was above 4 times as compared with that of acute stage. CONCLUSIONS: The results showed that the outbreak of acute conjunctivitis occurred in 1997 in Shenzhen was caused by adenovirus type 3.

Acute Disease↗

PubMed2000

[Comparison analysis on physical and value assessment methods for ecosystems services].

Physical assessment method (PAM) and value assessment method (VAM) for ecosystems services assessment were compared, and their strengths and weaknesses were analyzed. The comparison and analysis demonstrated that in some cases, different, even opposite results will be drawn with these two methods for the ecosystems services assessment of one ecosystem. The functions or effects of PAM and VAM are different for different assessment purposes or different spatial scales, but they will promote and complement each other to some extent.

Ecosystem↗

PubMed2000

[Forest ecosystem services and their ecological valuation--a case study of tropical forest in Jianfengling of Hainan Island].

This paper attempts to present forest ecosystem services and their indirect economic value of Jianfengling tropical forest in Hainan Island. The results show that average annual integrated ecosystem service value of Jianfengling tropical forest, which covers 44667.00 hm2, adds up to 664.38 million yuan(Chinese RMB), of which, about 71.64 million yuan is of the output of standing trees and other forest products, about 394.29 million yuan of water-holding, about 2.47 million yuan of soil conservation against erosion, about 13.16 million yuan of carbon fixation for reducing green house effect, about 4.29 million yuan of nutrient retention for N, P, K, Ca and Mg, about 178.53 million yuan of air purification.

Air↗

PubMed2000

Mannitolum infusion on cirrhotic patients with tense ascites treated by paracentesis.

OBJECTIVE: To investigate whether albumin can be substituted by mannitolum in cirrhotic patients with tense ascites treated by paracentesis. METHODS: Sixty-eight patients admitted to this therapeutic procedure were randomly assigned to receive intravenous albumin (36 patients) and mannitolum (32 patients) infusion. In repeated large-volume paracentesis (3-6 L/day), intravenous albumin 20 g or intravenous 20% mannitolum 250 ml were added. RESULTS: In 24 and 48 hours after paracentesis the mean value of electrolytes, liver and renal functions and various indicators of systemic circulation either in Group 1 or in Group 2 cases were found without changes (P > 0.05). As compared with that before paracentesis, the diameter of spleen vein was increased significantly (P < 0.05). The complications occurring after paracentesis were similar in both groups. CONCLUSIONS: It was suggested that paracentesis with intravenous infusion of mannitolum is an effective and safe method in treating cirrhotic patients with tense ascites.

Adolescent↗

PubMed2000

Effect of hormone replacement therapy on heart rate variability in postmenopausal women.

OBJECTIVE: To assess the characteristics of autonomic control of heart rate in postmenopausal women before and after hormone replacement therapy (HRT). METHODS: Time domain and spectral domain heart rate variability (HRV) were performed in 58 postmenopausal women before and after HRT. RESULTS: Compared with examinations at baseline, values of all HRV measurements in subjects given HRT were increased at the fourth month (P < 0.05), while they remained unchanged in the control group (P > 0.05). Results showed an inverse correlation between all measures of HRV and mean R-R interval in the treatment group. CONCLUSION: HRT reduces sympathetic drive in postmenopausal symptomatic women. HRV may be an index of imbalance of autonomic control in perimenopausal women.

Autonomic Nervous System↗

PubMed2000

Design and clinical application of cervical hollow threaded fixator.

OBJECTIVE: Cervical hollow threaded fixator (CHTF) was designed and applied to stabilize the cervical spine following anterior decompression due to cervical spondylosis or acute cervical disc herniation. METHODS: Medical use titanium alloy with no magnetism was made into a hollow threaded structure with holes on the wall of the fixator. Forty-two patients with cervical spondylosis and acute cervical disc herniation were stabilized using the CHTF technique after anterior decompression. X-ray films and CT scans were taken after the operation to observe the stability and fusion rate of the segments. RESULTS: Patients were allowed to walk one day after the operation and were followed up for 6 to 36 months. The operated segments were stable and solid fusion was observed 3-6 months after surgery. CONCLUSIONS: Immediate stability of the operated segments can be obtained using the CHTF technique. Patients were easy to manage after the operation and returned to their jobs in a short time. The CHTF technique is safe and simple to perform and eliminates the complications of an autograft. It may be an alternative to traditional cervical interbody fusion with bone graft.

Adolescent↗

PubMed2000

[Effect of kidney-replenishing herbs on ovarian function of dexamethasone-induced osteoporosis in rats].

OBJECTIVE: To investigate the effect of Kidney-replenishing herbs (KRH) on ovarian function of experimental rats with dexamethasone-induced osteoporosis (OP). METHODS: Radioimmunoassay was used to analyse blood level of growth hormone (GH), luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2), progesterone (P), testosterone (T) and prolactin (PRL) of animals and data of three groups, the normal control group, the pathologic control group (non-treated OP model) and the treated group (model rats treated with KRH) were compared. Weight and histomorphological features of ovary and uterus of the rats were also observed. RESULTS: As compared with the normal control group, levels of GH, LH, FSH, E2 and P in the pathologic control group were significantly lowered (P < 0.05), and the weight of ovary and uterus decreased (P < 0.05). In comparison of the treated group and the pathologic group, GH, LH, FSH, E2 and P levels were significantly higher and weight of ovary and uterus heavier in the treated group (P < 0.05). No obvious changes occurred in T and PRL levels. KRH showed markedly improving effect on the ovarian and uterine morphological construction of rats. CONCLUSIONS: KRH could elevate the level of GH, LH, FSH, E2 and P, increase the weight and improve the histomorphologic features of ovary and uterus in OP rats, it demonstrated that KRH has therapeutic effect in treating dexamethasone-induced OP rats.

Animals↗

PubMed2000

[Intracranial hemangiopericytoma 32 cases reports].

OBJECTIVE: To further elucidate the clinical feature, pathogenesis, treatment and prognosis of intracranial hemangiopericytomas (HPC). METHODS: Thirty-two cases of HPC treated in our department were analyzed retropectively. RESULTS: Grade I surgery, complet tumor removal with excision of involved dural and bone (7 cases); Grade II surgery, complet tumor removal with aggressively cauterization of dural and/or bone (13 cases); Grade III surgery, complet tumor removal but no satisfactory attempt to deal with involved dural or bone (7 cases); Grade IV surgery, tumor residual (7 cases). 13 cases were recurrent. CONCLUSION: The prognosis of intracranial hemangiopericytomas is dismal. The recurrent rates of HPC are very high. Microneurosurgery and adjuvant radiotherapy are main treatment. It is very important that counterchecking at regular intervals.

Adolescent↗

PubMed2000

[Operative therapy for lesions in insula].

OBJECTIVE: To probe into the significance of treating lesions in insula trans-sylvian approach in reducing cerebral injury in peri-tumor regions and post-operative complications. METHODS: All patients were operated on through fronto-temporal approach. The key procedure was that arachnoid membrane in the sylvian should not only be splited sharply under microscope but also be splited from the superface till to tumors in the insula. RESULTS: Post-operative CT showed no signs of cerebral injury. None of all the 15 patients suffered aphasia or memory change. CONCLUSION: Trans-sylvian approach is an effective operative way in treating lesions in insula.

Adult↗

PubMed2000

[Adenosine in treatment of rats with spinal cord injury].

OBJECTIVE: To observe the changes of endogenous extracellular adenosine after spinal cord injury (SCI) to rats and the effect of exogenous adenosine on extracellular calcium after SCI and post-injury neurological function. METHODS: A ventral compression injury model of T13 spinal cord was used, and the extracellular fluids were collected consecutively every 20 minutes after injury by using microdialysis. Adenosine in the samples was analyzed using high-pressure liquid chromatography (HPLC) with u.v. detection. The rats received different doses of 2-chloroadenosine (2-CADO), a nonspecific agonist of adenosine receptors, by intrathecal injection 15 minutes before injury. The extracellular fluid was collected every 10 minutes immediately after injury and the calcium was measured using atomic absorption spectrophotometer. Neurological function score, inclined plane angle, and histology were observed 24 hours after injury. RESULTS: A significant increase of adenosine was found immediately after spinal cord injury. The concentration of adenosine peaked at one hour after injury and dropped down to the basal level. There was a positive relation between the increase of adenosine and the severity of SCI. High dose of 2-CADO can significantly significantly inhibit the decrease of extracellular calcium and improve the neurological function of injured rats. CONCLUSIONS: Adenosine could involve the pathological process of secondary spinal cord injury and might play a protective role in SCI.

2-Chloroadenosine↗

PubMed2000

[Surgical treatment of single level unstable degeneration with foraminal stenosis].

OBJECTIVES: Lumbar spine single level unstable degeneration with foraminal stenosis was treated with unilateral decompression, disc excision, posterolateral interbody fusion utilizing single BAK cage and count-lateral facet joint screw fixation. The short-term and mid-term clinical results were evaluated. METHODS: There were thirty patients (13 male, 17 female) with an average age of 46.5 years. From posterior approach, all patients underwent unilateral decompression, disc excision, interbody fusion with posterolateral inserted single BAK cage, and count-lateral facet joint screw fixation. RESULTS: All patients underwent surgery safely without severe complications occurred such as infection and neurological damages. The average follow-up time was 12 months (6 - 18 months) with complete relief of symptoms. Solid fusion was achieved in all but 2 patients at final follow-up. 90% of the patients obtained successful clinical outcome. CONCLUSIONS: Complete decompression of the neural foramen can relief the symptoms, and interbody fusion utilizing threaded cage can restore the height of disc space and withstand axial loading of the spine. Addition of the facet joint fixation increased the postoperative segment stability and enhances solid fusion. With less invasive and preservation of part of the posterior elements of the lumbar spine and the solid fusion achieved, we consider it a sound procedure for the treatment of single level unstable degeneration with foraminal stenosis of lumbar spine. Long-term follow-up results needs to be observed.

Adult↗