Neural mechanisms mediating the hyperthermia elicited by prostaglandin E2 injected into the preoptic-anterior hypothalamus.
The neuromechanism mediating the hyperthermia induced by injection of PGE2 into the preoptic/anterior hypothalamus (PO/AH) was investigated in the rat. Pretreatment of rats with intraperitoneal injection of atropine sulfate blocked, whereas pretreatment with atropine methyl bromide had no significant effect on the hyperthermia. In a second series of experiments, atropine sulfate was microinjected into different regions of the hypothalamus and the thalamus in an attempt to locate the central cholinergic synapses involved in the PGE2-induced hyperthermia. The hyperthermia was blocked by atropine injection into the dorsal/dorsomedial hypothalamic area (DH), but was not significantly affected by injection into the PO/AH, ventromedial hypothalamus, or the thalamic area above the DH. Moreover, microinjection of the cholinergic agonist carbachol (0.5 microgram) into the DH could also elicit hyperthermia. Thus, our data suggest that the hyperthermia induced by PGE2 administration into the PO/AH is mediated by a cholinergic mechanism in the DH.