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J Z Liu

Publications and source records attributed to J Z Liu.

63 records · Page 4Linked to original sources
PubMed1986

Beta zero-thalassemia in association with a gamma-globin gene quadruplication.

We have studied the hematology, hemoglobin composition, and globin gene arrangements in one young Turkish boy with a beta zero-thalassemia homozygosity and in 11 of his relatives. Evidence is presented that the chromosome with the beta zero-thalassemia determinant carries a gamma-globin gene quadruplication, perhaps in a -G gamma-G gamma-G gamma-A gamma-gene arrangement. The eight gamma-globin genes in this patient produced G gamma and A gamma chains in a 95 to 5 ratio, and nearly 99% of the patient's hemoglobin was of the fetal type. The clinical condition resembled that of a thalassemia intermedia. HbF levels in eight beta-thalassemia heterozygotes varied between 0.5 and 4.2% and the percentages of G gamma in this HbF averaged at 87% or 95%; this level is to some extent related to the haplotype of the normal chromosome. All subjects carried four alpha-globin genes; a new BglII polymorphism was observed within the psi alpha-globin gene.

Consanguinity↗
PubMed1982

Studies on the mechanism of indirubin action in the treatment of chronic granulocytic leukemia. V. Binding between indirubin and DNA and identification of the type of binding.

The binding between indirubin and calf thymus DNA in vitro has been verified by means of the isotope labelling method, spectrophotometric method and thermal denaturation measurements. The lambda max 207 nm of indirubin shifted toward longer wave length with decrease of absorbance after the incubation of indirubin with DNA. The escalation of Tm value of DNA induced by indirubin was about 2.4 degrees C and it was reproducible. The binding force between them was rather weak, as indirubin molecules were easily released during the precipitation with alcohol or the gel filtration. The binding was not affected by sodium chloride even at high concentration but greatly decreased (to 20-30% of the control) in the presence of 8 M urea. These results showed that the binding between indirubin and DNA might be of hydrogen bond rather than ionic. The amount of bound 3H-indirubin was directly proportional to the concentration of indirubin. However, it increased abruptly when the concentration of indirubin reached 1.5 x 10(-4) M. This suggested another binding force in the latter instance. By using spectrophotometric analysis and Scatchard plot it was found that calf thymus DNA bound 46 indirubin molecules/1000 nucleotides. The association constant (K) was 5.7 x 10(6).

Animals↗
PubMedDate not supplied

Direct reverse transcription--polymerase chain reaction from whole blood without RNA extraction.

A simple, fast, efficient method of direct reverse transcription-polymerase chain reaction (RT-PCR) from whole blood without RNA extraction is described. After the erythrocytes in a few microliters of fresh blood were destroyed and removed, the total RNA was released from the leukocytes by means of water treated with diethyl pyrocarbonate. The solution was used as RNA template and directly introduced in the RT-PCR. Following this method, which is called direct RT-PCR, three different cDNA fragments were successfully amplified with three different pairs of primers, respectively. Meanwhile, the direct RT-PCR was compared with the RT-PCR from the extracted total RNA template. The results showed no obvious difference.

DNA, Single-Stranded↗