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Biomedical subjects

J Yoshida

Publications and source records attributed to J Yoshida.

At least 487 records · Page 27Linked to original sources
PubMed1980

Electrophoretic analysis of the protein in palatine saliva.

The protein in palatine saliva was investigated and compared with that in parotid saliva by means of SDS electrophoretic method. It was found that palatine saliva contains a large amount of high molecular weight protein (higher than 300,000) which is identified as mucin-rich glycoprotein and sialoglycoprotein, while no such protein is present in parotid saliva. The protein composition of palatine saliva has qualitatively smaller individual difference when compared to that of parotid saliva. We suggest that the high molecular weight protein which is specific to palatine saliva probably contributes to the rentention of complete maxillary dentures.

Adult↗
PubMed1980

Studies on a new aminoglycoside antibiotic, dactimicin. I. Producing organism and fermentation.

The producing organism of a new antibiotic, dactimicin, is described. The presence of finger-shaped sporangia bearing motile spores, the absence of aerial mycelium and its cell wall of type II, ascribe this organism to the genus Dactylosporangium. The cultural and physiological features distinguish this organism from all the described Dactylosporangium species. Therefore, it is considered to be a new species for which the name Dactylosporangium matsuzakiense sp. nov. is proposed. Fermentation of dactimicin is also described.

Aminoglycosides↗
PubMed1980

In vitro transformation of fetal brain cells from CDF rats exposed in utero to N-ethyl-N-nitrosourea: morphologic and immunologic studies.

Inbred CDF rats received 250 mg N-ethyl-N-nitrosourea (ENU)/kg body weight in monosodium phosphate buffer (pH 5.5) during late pregnancy (18-21 days). Control rats received only buffer. One hour after ENU or buffer administration, the fetuses were delivered and fetal brain cells (FBC) were put in long-term tissue culture. Cell growth consisted of monolayers of glial, fibroblastic, and epithelial-like cells. FBC from buffer-treated rats could not be subcultured for more than 13-15 passages; only one culture was maintained up to the 26th passage. FBC from ENU-treated rats showed in vitro malignant transformation beginning at the 8th passage (100 days). Injection of these cells sc into irradiated or neonatally thymectomized syngeneic rats induced small palpable tumors that regressed completely by 15-28 days. Small nonprogressively growing subcutaneous tumors in normal rats, however, were obtained only after the 19th in vitro passage (200 days). These transformed FBC appeared highly antigenic. Tumor-associated antibody was demonmmune adherence assays. Rats immunized with irradiated malignant FBC showed rising tumor antibody titers. The antibody was absorbed by ENU-exposed FBC but not by normal fetal or adult syngeneic brain cells. We concluded that ENU-exposed cells become malignant when grown in vitro for at least 8 passages (100 days) but grow in vivo in X-irradiated or neonatally thymectomized rats only. These antigenic and tumorigenic clones may be selected in vitro, where they grow readily in the absence of immune resistance.

Animals↗
PubMed1979

[Reproduction study of 4-ethoxy-2-methyl-5-morpholino-3(2H)-pyridazinone (M73101) in rabbits. Administration of M73101 during the period of major organogenesis (author's transl)].

M73101 and aspirin were evaluated for the effects on prenatal development of progeny in JW-NIBS strain rabbits. Pregnant females were given orally M73101 (0, 50, 120 and 300mg/kg/day) or aspirin (200mg/kg/day) on days 6 to 18 of gestation. They were sacrificed on days 29 of pregnancy and their fetuses were examined for abnormalities. The results were summarized as follows: 1. During the dosing period, females received 300mg/kg of M73101 showed pronounced body weight depression and decrease in food and water intake. But after the dosing period, these influences were not seen. Females received aspirin did not show such phenomena. 2. There were no adverse effects on number of implants, litter size, fetal mortality or fetal weight, and no malformed fetuses attributable to the drugs were seen in each group.

Abnormalities, Drug-Induced↗
PubMed1979

[Reproduction study of 4-ethoxy-2-methyl-5-morpholino-3(2H)-pyridazinone (M73101) in rats (II). Administration of M73101 during the period of major organogenesis (author's transl)].

Pregnant female rats were given orally M73101 (0,100,250 and 600 mg/kg) or aspirin (225 mg/kg) on gestational days 7 to 17. About two-thirds of treated females in each group were sacrificed at day 21 of pregnancy and their fetuses were examined for abnormalities. The remaining females were allowed to deliver spontaneously in order to observe postnatal development of their offspring. The results were summarized as follows: 1. No significant effects of M73101 on number of the corpora lutea, litter size, fetal mortality, fetal weight or sex ratio were observed, but aspirin exhibited the intrauterine deaths and growth retardation in fetuses. 2. No external, internal or skeletal malformations attributable to M73101 were seen in fetuses, although aspirin caused malformations of various organ systems. 3. No apparent effects of M73101 on F1 generation were observed on postnatal development including emotionality, learning ability and reproductive performance. There were no adverse influences of M73101 on postnatal development of F2 generation. Aspirin, however, showed adverse effects on postnatal survival, growth, emotionality and mating performance of F1 generation.

Abnormalities, Drug-Induced↗
PubMed1979

Studies on Actinomycetales producing antibiotics only on agar culture. I. Screening, taxonomy and morphology-productivity relationship of Streptomyces halstedii, strain SF-1993.

Soil Actinomycetales that produce antibiotics during growth on agar but not in submerged culture were searched for and about 25 strains were obtained. One of these, Streptomyces halstedii, strain SF-1993, which produces an antibiotic designated SF-1993 was studied taxonomically and morphologically. The antibiotic-productivity of strain SF-1993 was correlated with mycelial morphology. The vegetative mycelium was filamentous in antibiotic-producing agar cultures, but fragmented in non-producing submerged cultures. By maintaining submerged cells filamentous, production of antibiotic in the submerged fermentations was accomplished. Filamentation was maintained by the use of diluted media or non-fragmented mutant substrains.

Agar↗
PubMed1978

Enhancement of 5-hydroxytryptamine-induced contraction of the guinea pig ileum by a new sulfur compound, tripropylsulfonium bromide.

In the presence of tripropylsulfonium bromide (TPS) (1 X 10(-4) g/ml), a new compound, the phasic contraction of the isolated guinea pig ileum to 5-hydroxytryptamine (5-HT) (5 X 10(-7) g/ml) was consistently enhanced ("TPS effect"). TPS alone increased moderatley the spontaneous movement of the ileum. When the contraction height was calculated as the percentage of that to 5-HT alone, such was observed in the "TPS effect" to be 167.1 +/- 3.6% (mean +/- S.E., n = 80). TPS did not enhance the contraction due to acetylcholine or histamine. The "TPS effect" remained unaffected in the presence of dibenzyline (1 X 10(-7) g/ml), was abolished by morphine(1 X 10(-6) g/ml), tetrodotoxin (2 X 10(-8) g/ml) adenosine (3 X 10(-6) g/ml) and atropine (1 X 10(-7) g/ml) and was not observed under anoxic conditions. Eserine (1 X 10(-8) g/ml) strengthened the "TPS effect" markedly. It is concluded that this effect may be the result of the potentiating effect of TPS on the action of 5-HT through the M receptors, possibly by the facilitation of the acetylcholine-liberation from the nervous tissue.

Acetylcholine↗