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Biomedical subjects

J Yano

Publications and source records attributed to J Yano.

121 records · Page 7Linked to original sources
PubMed1976

The EEG response to repetitive photic stimulation in various regions of the chicken brain.

The EEG response in the chicken to repetitive photic stimulation was studied by frequency analysis and by the averaged response. Evoked responses were observable not only in stations along the visual pathway but also in broad areas apart from the visual pathway. The electrical activity in the archistriatum showed a marked response to flickering stimuli, indicating that this area is involved in the visual function in the chicken. In other telencephalic areas, photically evoked potentials could not be clearly demonstrated in the EEG records. In the hypothalamus and the nucleus rotundus of the diencephalon and in the nucleus reticularis superior of the mesencephalon, sinusoidal waves appeared during stimulation at 8-13/sec. No rhythmic after-discharge was observed following termination of photic stimulation. These finding are indicative of the difference of the visual response in the chicken from previously reported responses in other species.

Animals↗
PubMed1974

Seven-membered cyclic phosphate: synthesis and properties of S-cycloadenosine 2',5'-cyclic phosphate and cordycepin 2',5'-cyclic phosphate. Studies of nucleosides and nucleotides. LXIV. 1). Purine cyclonucleosides 25.

8,3'-Anhydro-8-mercapto-9-beta-D-xylofuranosyladenine (8,3'-s-cycloadenosine) was phosphorylated with cyanoethyl phosphate and DCC to 5'-phosphate. After 6-amino group was benzoylated, the monophosphate was treated with DCC to give a cyclic phosphate (II). The structure of compound II was elucidated as 8,3'-s-cycloadenosine 2',5'-cyclic phosphate by UV, NMR and CD spectra, as well as enzymatic hydrolyses. When compound II was desulfurized with Raney nickel, cordycepin 2',5'-cyclic phosphate (III) was obtained. Although compound III could be obtained from cordycepin 5'-phosphate with DCC, the yield was extremely low.

Benzyl Compounds↗
PubMedDate not supplied

Introducing antisense oligodeoxynucleotides into Paramecium via electroporation.

A method utilizing electroporation to deliver antisense oligodeoxynucleotides into Paramecium tetraurelia has been developed. For these studies antisense oligonucleotides directed to different regions of the calmodulin mRNA were used. It was found that a pulse delivered at 150-250 V (375-625 V/cm field strength) for 3.9-4.2 ms using a 275 microF capacitor with resistance set at 13 Ohms was sufficient to achieve measurable incorporation of fluorescently-labeled oligodeoxynucleotides in up to 95% of the cells treated. Optimal parameters included using oligodeoxynucleotides of at least 12 bases in length with a 3' blocking group at a dose of around 10 microM. In addition, multiple oligodeoxynucleotides directed to the same target mRNA resulted in at least a 10-fold reduction in the dose of oligodeoxynucleotide required to achieve the desired effects. Taken together, these results indicate that the use of antisense oligodeoxynucleotides can be an easy and useful method for linking genes to specific functions in Paramecium tetraurelia. Finally, this report discusses how different 3' blocking groups and the use of combinations of oligodeoxynucleotides directed to different regions of the same target mRNA can help address concerns about specificity.

Animals↗