Biomedical subjects

J Y Yang

Publications and source records attributed to J Y Yang.

At least 109 records · Page 6Linked to original sources

PubMed1991

Free flap transfer in burn reconstruction.

Wound healing in burn patients with exposure of vital tissues has been greatly facilitated by the use of free flap transfers for reconstruction with good functional as well as aesthetic results. Preoperative angiography is not an indispensable way to localize recipient vessels. Knowledge of the changes that occur in vessels at the zone of injury is crucial in the selection and preparation for microsurgical anastomosis. One team approach is advocated because the plan for the procedure may be changed when no appropriate recipient vessel is located during the dissection. We present our study of ten cases followed up in the last six years. Nine cases were successful, and severe wound infection resulted in one flap failure. Proper recipient vessel selection and adequate wound debridement are important factors for successful free flap transfer in burn reconstruction.

Adult↗

PubMed1990

Cloning and sequence analysis of the cDNA for human placental NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase.

NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase catalyzes the oxidation of many prostaglandins at C-15, resulting in a subsequent reduction in their biological activity. We report the isolation of the cDNA for this enzyme. A human placental lambda gt11 cDNA library was screened using polyclonal antibodies prepared against the human placental enzyme. A 2.5-kilobase cDNA containing the entire coding region for the enzyme was isolated. The cDNA encodes for a protein of 266 amino acids with a calculated Mr of 28,975. Identification of the cDNA as that coding for 15-hydroxyprostaglandin dehydrogenase was based on the comparison of the deduced amino acid sequence with the amino acid sequence of two peptides, one from the rabbit lung enzyme and the other from the human placental enzyme. This cDNA hybridizes with two species of poly(A+) RNA isolated from human placenta: one of 3.4 kilobases and the other of 2.0 kilobases. Isolation of the cDNA for 15-hydroxyprostaglandin dehydrogenase should facilitate studies on the structure, function, and regulation of this enzyme.

Amino Acid Sequence↗

PubMed1990

Effect of wet decomposition methods on the determination of cobalt, copper, selenium, and zinc in biological samples using electrophoresis.

A method combining radiotracer techniques with paper electrophoresis to investigate the optimal decomposition conditions for cobalt, copper, selenium, and zinc in rat liver samples is described. After oral and intraperitoneal administration of the respective nuclides of 60Co, 64Cu, 75Se, and 65Zn solutions, the livers of the tested rats are removed and decomposed with a nitric and sulfuric acid mixture. The completeness of decomposition is investigated by measuring the respective radionuclide-containing species in the decomposed samples by electrophoretic analysis. The results indicate that the Co- and Se-containing liver samples are more easily decomposed than those containing Cu and Zn under the specific conditions. The same decomposition effects are observed for ingested radioisotopes as for intraperitoneally injected ones. The possible connection of decomposition with the analytical error is discussed.

Animals↗

PubMed1990

Clinical application of collagen sheet, YCWM, as a burn wound dressing.

In the Linkou Burn Unit, a recently developed new wound dressing derived from porcine skin was evaluated. This newly designed porcine dressing, called young collagenous wettable membrane (YCWM), was developed by the staff of the Department of Cellular and Molecular Biology of Chang Gung Memorial Hospital. Many specific characteristics, such as negligible antigenicity, semitransparency, sterilizability, good pain relief and low costs, have been associated with it. The results of a clinical trial on 59 wounds on 50 burn patients proved encouraging. Some disadvantages such as maceration and delayed eschar separation were noted. We conclude that YCWM is suitable for the treatment of the clean, dry donor sites and superficial partial skin loss burn wounds of non-infected, non-immunocompromised patients.

Biological Dressings↗

PubMed1990

Lyme disease presenting as urinary retention.

We report a case of Lyme disease that presented with urinary retention. The individual then experienced lower extremity paralysis. Paralysis and urinary retention resolved with intravenous ceftriaxone antibiotic. To our knowledge this is the first report of a urological manifestation as the initial clinical presentation of Lyme disease. Recognition of clinical symptoms and urological complications of Lyme disease also are discussed.

Adult↗

PubMed1990

[Combined pharmacokinetic--pharmacodynamic model analysis of N-acetyl procainamids following intravenous infusion in rabbits].

The pharmacokinetic and pharmacodynamic profiles of N-acetyl procainamide were analyzed by integrated PK-PD model following intravenous infusion to rabbits. No significant differences between the PK parameters estimated from iv administration and intravenous infusion were found. However, two of the PD parameters were shown to be significantly different. The values of Emax, Keo, S, EC50 were found to be 120 +/- 13.2 ms, 0.0182 +/- 0.007 min-1, 2.26 +/- 0.93, 6.31 +/- 0.71 microgram/ml respectively following intravenous infusion; the corresponding values following iv administration were 53.6 +/- 2.5 ms, 0.061 +/- 0.017 min-1, 2.19 +/- 0.39, 6.21 +/- 1.74 micrograms/ml respectively.

Acecainide↗

PubMed1989

Pediatric burns--2 year survey of cases admitted to the Chang Gung Memorial Hospital.

A total of 722 patients were admitted to the Burn Unit of Chang Gung Memorial Hospital over a 2-year period, of these 285 (39.5%) were under 15 years of age. The major cause of pediatric burns was scalding, 236 (82.8%). Most of the patients under age of 15 years had total burn surface under 20% (199 patients, 70%), and were discharged from the hospital within one month (263 patients, 91%). Treatments included debridement done in 98 patients (41.5%) and skin grafting in 81 patients (28%). Oxacillin-Resistant Staphylococcus Aureus infections were the most common complication (5.3%). The mortality rate was 5.3%.

Adolescent↗

PubMed1989

Experience of reverse medial arm flaps in the reconstruction of burned elbow scar contractures.

There are many methods to correct scar contractures in the elbow region after burn injury, including Z-plasty, Y-V or rectangular flaps, local or distant fasciocutaneous flaps, muscle or myocutaneous flaps, free flaps, tissue expanders and non-surgical orthotics. Among these, the reverse medial arm island flap, based on the recurrent ulnar artery, has proved to be a convenient local fasciocutaneous flap for elbow scar reconstruction. In the past 2 years, 12 reverse medial arm flaps were used in 11 patients. The results are satisfactory. Two complications, one partial flap necrosis, another ulnar nerve compression, were noted. Our clinical experience with this flap encourages us to use it for burn elbow reconstruction in the future. The advantages and disadvantages are discussed.

Adult↗

PubMed1989

Clinical comparison of commercially available Biobrane preparations.

This prospective clinical comparison of the three commercially available Biobrane preparations indicated that: (1) All three products of Biobrane are excellent skin substitutes. (2) Regular Biobrane has satisfactory wound adherence, however, its non-porous structure allows limited wound drainage. Because of this, it has the highest incidence of haematoma and fluid accumulation (13.3 per cent) and delayed epithelialization (18.8 per cent) of the three products. (3) Thin Porous Biobrane has poor adherence which limits patient activity and provides poor pain relief. The infection rate of 10 per cent was the highest of the three products. (4) Regular Porous Biobrane demonstrated superiority to the other two Biobrane products in this study. It provided good wound adherence while maintaining wound drainage because of its porous structure. The incidence of blood or fluid accumulation (7.1 per cent) was the lowest in the three products. (5) Most of the complications such as infection, delayed epithelialization, pain and activity impairment were related to fluid or blood accumulation. Adherence was found to be more important and reliable than pore structure. Operative haemostasis thus should be emphasized when using porous Biobrane, as with all skin substitutes.

Adolescent↗

PubMed1989