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J Wojcierowski

Publications and source records attributed to J Wojcierowski.

33 records · Page 2Linked to original sources
PubMed1995

[Fibronectin gene expression in preneoplastic states and female genital neoplasms].

In this paper the fibronectin gene expression level (Northern blotting) in 33 endometrial samples (residua post abortum, endometrium proliferativum, secretivum and involutivum, hyperplasia glandularis endometrii, adenocarcinoma endometrii) was measured and compared. Higher level of fibronectin gene expression level in endometrial hyperplasia and carcinoma than in other endometrial samples was found. Strong correlation between high fibronectin gene expression level in endometrial hyperplasia and carcinoma was found.

Blotting, Northern↗
PubMed1989

Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.

The monoclonal antibody-defined, tumor-associated antigen GA733 was purified from the SW948 human colorectal carcinoma cell line and its partial amino acid sequence was determined. By using a synthetic oligonucleotide probe, two recombinants were isolated from a total human genomic library. We prove the existence of a family of GA733 genes. One of the genomic isolates is demonstrated to be an intronless gene, which is transcribed in pancreatic carcinoma cell lines and in placenta. The GA733 proteins were observed to contain sequences homologous to a repeat unit occurring 10 times in thyroglobulin and once in the HLA-DR-associated invariant chain. A more evolutionarily distant relationship was found with the alpha chain of the interleukin 2 growth factor receptor.

Amino Acid Sequence↗
PubMed1987

Transcription expressed by isolated cell nuclei in relation to the cell cycle.

In vitro transcription of stimulated T lymphocyte nuclei was studied using a previously described method for evaluating the accumulation of double labelled transcription products. Whereas RNA elongation was inhibited during the G0 and S phases of the cell cycle, the synthesis of short-living products (probably products of abortive initiation) still proceed in vitro. Release of the elongation block with concommitant decrease of short-product synthesis was observed during the G1 phase.

Cell Cycle↗
PubMed1986

Method of double labelling applied to studies of transcription in isolated rat liver nuclei.

Nonlinear transcription in isolated cell nuclei was observed and elucidated. The method of double labelling of acid insoluble products was applied. Labelled precursors of RNA 14C-CTP and 3H-CTP were introduced separately into incubation media for different times. The parameters of reaction (time of the average template transcription, reinitiation frequency and rate of depolymerization) were optimized by computer. It is suggested that two different types of product are formed, namely 'long' for which the time of synthesis was sometimes longer than 90 min, and 'short' which were sized in less than 5 min. These products were different in respect of both their size and turnover.

Animals↗
PubMed1985

Interindividual phenotypic variations in CLL B lymphocyte maturation indication of different maturation blocks.

Peripheral blood lymphocytes from 8 patients with B-derived chronic lymphocytic leukaemia were stimulated by Staphylococcus aureus bacteria strain Cowan 1 with T cell mitogens PHA or PWM in 5-7 day suspension cultures. For the first group of patients proliferation and maturation tests were performed on T cell enriched and T cell depleted subpopulations, obtained from harvested lymphocytes at the end of cultures by the sheep red cell rosette technique. To re-examine mutual influences of cultivated T and B cells in the second group of experiments, lymphocytes from CLL patients and from 5 healthy individuals were investigated by the use of transmembrane cocultivation system after Feldman and Basten. The proliferative responses to lectin and to bacteria were assessed by 3HTdR-blastic and mitotic indices. The maturation process of B lymphocytes was examined by cytoplasmic Ig, studied by FITC-conjugated antisera. Results obtained with cocultivation system support the view that T cell replacing factor(s) were required for inducing prolonged growth and development of maturation of more numerous B lymphocytes in response to Staphylococcus aureus, a T cell independent, B specific polyclonal stimulator. Results analysed in different patients indicate various degrees of maturation of B cells including their differentiation towards a plasmacytoid cell, accompanied by various proliferative capacities of B and T lymphocytes. This functional analysis reflects the heterogeneity of B-CLL patients group.

Adult↗
PubMed1976

RNA synthesis in nuclei of rat liver and of human lymphocytes.

Physico-chemical properties and RNA synthesis in the rat liver and human lymphocytes have been compared in a nuclear system in vitro. Human lymphocytes were isolated from blood of healthy donors and of chronic lymphocytic leukaemia patients. The isolated nuclei served as the source of polymerase and template DNA. 3H-CTP was incorporated into the acid insoluble fraction linearly for 60 min. The nuclei of lymphocytes contained small amounts of RNA and protein, and the isolation procedure was complicated. Rat liver nuclei seem to be less prone to clumping at high pH values and may incorporate much more 3H-CTP. The nuclear synthesis was compared with incorporation of 3H-rU and 32P-orthophosphate into nuclear RNA of intact lymphocytes. Normal cells easily incorporated 32-P, and in contrast leukaemic cells incorporated 3H-rU to a greater extent.

Animals↗
PubMedDate not supplied

Detection of CFTR gene mutations in patients suffering from chronic bronchitis.

BACKGROUND: The purpose of the study was to examine cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in patients suffering from chronic bronchitis. METHODS: Thirty-two patients admitted to the Department of Pulmonology, Lublin School of Medicine, Lublin, Poland between 1995 and 1996 due to chronic bronchitis were included in the study. Patients were analyzed for the eight most common mutations of the CFTR gene (DeltaF508, G542X, N1303K, 1717-1(GoA)), W1282X, G551D, R553X, and DeltaI507 by the reverse-hybridization method. RESULTS: CFTR gene mutations were found in five of 32 (16%) patients, all within the DeltaF508 region of the CFTR gene. All positive samples were obtained from patients heterozygous for the DeltaF508 mutation. The presence of the DeltaF508 mutation was considered statistically significant when our study group was compared to the study of Poland's general population (p <0.05 Fisher's exact test). CONCLUSION: Our results suggest there is an increased presence of the DeltaF508 point mutation of the CFTR gene in Polish patients suffering from chronic bronchitis.

Adolescent↗
PubMedDate not supplied

Detection of human papillomavirus types 16 and 18 in human neoplastic endometrium: lack of correlation with established prognostic factors.

HPV (types 16 and 18) DNA sequences are present in the majority of precancerous and cancerous lesions of the human uterine cervix. However, data concerning the involvement of HPVs infection in the pathogenesis of endometrial cancer are controversial. In the current study we investigated the frequency of the HPV types 16 and 18, detected by PCR amplification using the type 16- and 18-specific primers within the E7 Open Reading Frame (ORF) sequence, in 54 human endometrial carcinomas obtained from women of Polish origin. Moreover, we assessed the possible association of the HPV with the clinicopathological features of the cancer, patients' outcome as well as with the K-ras codon 12 gene point mutations. HPV type 16 was present in eleven out of 54 (20%) endometrial tumors, while HPV type 18 was detected only in three out of 54 (4%) neoplasms analyzed. HPV infection was not related either to the patients' age (r=0.11; p=0.428, Spearman correlation test) or to the clinicopathological parameters and patients' prognosis. A higher incidence of HPV 16/18 was detected in well (G1) differentiated than in moderately (G2) and poorly (G3) differentiated endometrial adenocarcinomas, but the difference was not statistically significant. Moreover, none of HPV-positive endometrial carcinomas harbored K-ras codon 12 gene point mutations. Our results suggest that some of the endometrial carcinomas are associated with HPV infection but the presence of the human papillamovirus types 16/18 is not related to the clinicopathological or prognostical features of the neoplasm.

Adenocarcinoma↗
PubMedDate not supplied

[Neurofibromatosis type 1 --Recklinghausen's disease; pathogenesis and clinical symptoms].

Neurofibromatosis type 1 (NF1) or von Recklinghausen neurofibromatosis is a common autosomal dominant disorder affecting 1 in 3000 individuals. The gene for NF1 is localized on chromosome 17q11.2. The gene mutations or the inactivation its protein product--neurofibromin are responsible for the manifestation of the disease. NF1 demonstrates a wide variability of clinical symptoms classified by NIH Consensus Conference in 1987.

Chromosomes, Human, Pair 17↗
PubMedDate not supplied

[Use of polymerase chain reaction for pediatric diagnosis].

Basic principles of main molecular biology techniques: polymerase chain reaction (PCR) and its variants; asymmetric PCR, ARMS, nested PCR, multiplex PCR and competitive PCR are discussed. Nucleic acids techniques are making increasing progress in molecular diagnostics. Sequence analysis of amplified DNA allows better identification of the pathogen, detection of mutant genes and more accurate prognosis and therapy of certain diseases.

Child↗