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Biomedical subjects

J Watson

Publications and source records attributed to J Watson.

At least 451 records · Page 25Linked to original sources
PubMedDate not supplied

Chromatographic investigation of the glycosylation pattern of alpha-1-acid glycoprotein secreted by the HepG2 cell line; a putative model for inflammation?

In certain pathophysiological conditions, such as rheumatoid arthritis, there are alterations in the glycosylation pattern of the acute phase protein, alpha-1-acid glycoprotein (AGP). These changes are likely to be functionally significant, however, verification of the latter role requires a system which reflects in vivo glycosylation changes in AGP and also produces sufficient quantities of the protein for further study. The human hepatoma cell line HepG2 is documented as displaying a shift in the glycosylation pattern of glycoproteins from normal state to acute phase after stimulation with inflammatory mediators. We have isolated AGP from the culture medium of HepG2 cells both before and after stimulation with a cytokine preparation and analysed the glycosylation pattern of each preparation, after enzymatic release, by high pH anion-exchange chromatography. Before stimulation, the glycosylated population was similar to a profile of AGP isolated from normal plasma; however, cytokine stimulation resulted in a shift to a profile which was consistent with that of AGP from a rheumatoid arthritis sufferer. Thus a HepG2 cell culture system is capable of being a crude model of the changes in glycosylation of acute phase proteins although it has a tendency to produce oligosaccharide chains which are not fully sialylated.

Animals↗
PubMedDate not supplied

Efficacy of out of hospital defibrillation by ambulance technicians using automated external defibrillators. The Heartstart Scotland Project.

During the Heartstart Scotland project all 407 ambulances in Scotland were equipped with automated external defibrillators (AEDs). All cases of chest pain or collapse aged over 10 years were monitored and multiple 3-s rhythm strips recorded in a solid state memory module. A shockable rhythm was defined as an organised rhythm of > or = 180 beats/min or a disorganised rhythm of > or = 100 beats/min and amplitude > 0.1 mV. We analysed all the stored rhythm strips in two patient populations to determine the ability of the AED and ambulance crews to detect a shockable rhythm and to initiate appropriate defibrillation. The first population comprised 493 patients, all of whom had received shocks. A total of 4741 rhythm strips were analysed, of which 1461 were true positives, 33 false positives, 3161 true negatives and 86 false negatives. Overall sensitivity of the AED was 94.4% and specificity 99.0%. The second population comprised a random sample of 200 shocked and 200 non-shocked arrests. The combined group contained 4154 rhythm strips of which 562 were true positives, 12 false positives, 3460 true negatives and 120 false negatives. Overall sensitivity of the system (AED+crew) was 82.4% and specificity 99.7%. However, only 66 of the 120 false negatives were attributable to the AED giving a sensitivity of 90.3% for the AED. The sensitivity of the AED is dependent on the prevalence of shockable rhythms, but will be within the range 90.3-94.4% for most emergency medical services. We conclude that early management of potentially lethal arrhythmias by ambulance technicians using AEDs is practical with acceptable sensitivity and specificity.

Ambulances↗
PubMedDate not supplied

An in vitro comparison of biofilm formation on various biliary stent materials.

Stent occlusion may occur as the result of bacterial adherence and biofilm formation. This occlusion may be prompted by the material or design of the stents. In this study, a bile perfusion model was used to compare the biofilm formation on various stent materials. The copolymer and the wire mesh stents demonstrated less biofilm formation than the traditional stent. The development of new polymers may prolong stent patency.

Bile Ducts↗
PubMedDate not supplied

Reduced Kamin blocking in non paranoid schizophrenia: associations with schizotypy.

Kamin blocking (KB) is an attentional phenomenon whereby prior learning about a stimulus (A) retards learning about a new stimulus (B) when later presented in compound (AB) with the original stimulus A. KB has been shown to be reduced in patients with schizophrenia. Using Oades' KB paradigm it has been suggested that drug treatment may influence the expression of KB abnormalities in patients. It is therefore unclear whether Reduced KB are due to drug treatment or to the illness itself. One experimental approach that circumvents drug treatment confounds is to study schizotypal traits in healthy volunteers. In the present study we investigated KB using the Oades paradigm in 27 healthy volunteers and 21 schizophrenic patients. We additionally investigated the relationship between KB performance and measures of schizotypal traits and a number of factors relevant to the experience of schizophrenia using the O-LIFE questionnaire. Our results indicate first a clear negative relationship between general schizotypy and more specifically, Unusual experiences (UNEX) and cognitive disorganisation (COGDIS) and KB performance. This relationship was qualitatively and quantitatively similar in both healthy volunteers and schizophrenic patients. Second we have independently replicated reduced KB in non-paranoid patients and no change in KB in paranoid patients using the Oades KB task. This study also confirms that reduced KB in non-paranoid patients is confined to early test trials (3-4) while the negative relationships with schizotypy scales UNEX and COGDIS that we have found are also confined to these early test trials confirming the psychological relevance of this specificity.

Adult↗
PubMedDate not supplied

New World cutaneous leishmaniasis in returned travellers: treatment failures using intravenous sodium stibogluconate.

Treatment outcome was determined among a cohort of travellers who returned to the UK between February 2000 and February 2001 with New World cutaneous leishmaniasis caused by species of the Leishmania (Viannia) subgenus. Among 18 patients who completed treatment with 20 mg/kg/d of i.v. sodium stibogluconate (NaSb) for 20 d, early relapse of disease occurred in 2 patients with regional dissemination in 1 and mucocutaneous involvement in the other. Drug susceptibility testing in vitro of the clinical isolate from 1 of these patients confirmed tolerance to high concentrations of NaSb.

Adult↗
PubMedDate not supplied

Missing annual external radiation dosimetry data among Hanford workers.

Epidemiological studies of workers employed at the Hanford Site have been underway for nearly 30 years. Although the external radiation dosimetry program at Hanford has been fairly comprehensive, some workers included in previous epidemiological analyses have periods of employment during which there are missing annual external radiation dosimetry records. In this report, employment history records and annual external dosimetry records have been used to investigate the extent of missing annual external dosimetry records for workers at the Hanford facility. A "nearby" procedure for estimating values for missing annual external dosimetry records was evaluated. Among the 33,459 workers who were employed at least 180 days and had at least one annual external dosimetry record, annual external dosimetry records were missing for 8% of the years of employment (32,323 missing annual external dosimetry records). Missing annual external dosimetry records were more common for female Hanford workers than for male workers, and for workers employed in the early years of Hanford's operation than for workers employed in later years of operation. The nearby procedure provided reliable estimates of values for missing annual external dosimetry records. Using this procedure, 18,937.5 mSv were estimated for missing annual external dosimetry records; this was 2% of the total recorded cumulative external radiation dose for these workers. Missing annual external dosimetry records should be considered as a potential source of bias and uncertainty in investigations of radiation-cancer associations among Hanford workers.

Air Pollution, Radioactive↗
PubMedDate not supplied

Evaluation of annual external radiation doses at values near minimum detection levels of dosimeters at the Hanford nuclear facility.

In epidemiological studies of workers exposed to ionizing radiation, recognition of the limitations of available radiation dosimetry data is important to interpretation of a study's findings. This paper provides an investigation of external radiation dosimetry data for workers at the Hanford nuclear facility, focusing on changes over time in practices for recording dosimetry measurements that were between zero and the minimum detectable level of a radiation dosimeter. Reported annual external radiation doses for the years 1944-1989 were examined for 33,459 workers; these records are the sum of periodic dosimetry measurements within a calendar year. For each year, the proportion of annual external doses with values in the range of minimum detectable level was examined. Contrary to previous researchers, we conclude that there is evidence that dosimetry measurements with values between zero and the minimum detectable level may have been recorded as zero in some historical periods. This conclusion is supported by evidence drawn from historical documentation about radiation dosimetry practices at the Hanford facility. Although workers at the Hanford facility have relatively complete and detailed external radiation dosimetry data compared to some other nuclear facilities that began operation in the 1940s, these data may suffer limitations related to dosimetry recording practices at the facility.

Adult↗
PubMedDate not supplied

The prevalence of attentional problems and the effect of methylphenidate in children with myelomenigocele.

The prevalence of attentional problems, and the effect of methylphenidate was evaluated in a clinic population of children with myelomeningocele. Families of 79 children between the ages of 6 and 15 years were screened for the presence of attention problems in their children, using Conners' questionnaires for parents and teachers, and/or the DSM-IV checklist. Thirty-nine per cent of the children exhibited attention problems, primarily without hyperactivity. Fourteen children with attentional problems were enrolled in a double-blind placebo-controlled trial methylphenidate. Response to methylphenidate was assessed with Conners' questionnaires, Conners' Continuous Performance Test, and a battery of selected neuropsychological tests. No statistically significant response was measured for the group while on methylphenidate. Four children were clinical responders to methylphenidate. The prevalence of attentional problems in children with myelomeningocele is high, and effective medication therapy needs to be studied further.

Adolescent↗
PubMedDate not supplied

Meta-analysis of theory-of-mind development: the truth about false belief.

Research on theory of mind increasingly encompasses apparently contradictory findings. In particular, in initial studies, older preschoolers consistently passed false-belief tasks-a so-called "definitive" test of mental-state understanding-whereas younger children systematically erred. More recent studies, however, have found evidence of false-belief understanding in 3-year-olds or have demonstrated conditions that improve children's performance. A meta-analysis was conducted (N = 178 separate studies) to address the empirical inconsistencies and theoretical controversies. When organized into a systematic set of factors that vary across studies, false-belief results cluster systematically with the exception of only a few outliers. A combined model that included age, country of origin, and four task factors (e.g., whether the task objects were transformed in order to deceive the protagonist or not) yielded a multiple R of .74 and an R2 of .55; thus, the model accounts for 55% of the variance in false-belief performance. Moreover, false-belief performance showed a consistent developmental pattern, even across various countries and various task manipulations: preschoolers went from below-chance performance to above-chance performance. The findings are inconsistent with early competence proposals that claim that developmental changes are due to tasks artifacts, and thus disappear in simpler, revised false-belief tasks; and are, instead, consistent with theoretical accounts that propose that understanding of belief, and, relatedly, understanding of mind, exhibit genuine conceptual change in the preschool years.

Child↗