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Biomedical subjects

J Watson

Publications and source records attributed to J Watson.

At least 271 records · Page 15Linked to original sources

Progesterone production by human ovarian tissues in response to deglycosylated human chorionic gonadotrophin.

Removal of carbohydrate residues from human chorionic gonadotrophin is known to reduce its biological activity in the ovaries of monkeys, pigs and rats although its binding properties appear unaffected. The present study demonstrates that such modified hCG derivatives also appear to have impaired biological activity in human ovaries in that they do not significantly stimulate progesterone secretion by follicular granulosa cells in culture or corpus luteum slices during superfusion. The derivatives also appear to reduce the normal stimulatory action of hCG when incubated with it.

Chorionic Gonadotropin↗

Plasma morphine concentrations and analgesic effects of lumbar extradural morphine and heroin.

Patients undergoing lumbar laminectomy were given extradural narcotic, either 5 mg morphine sulphate or 5.5 mg heroin (diamorphine hydrochloride); the extradural catheter had been positioned adjacent to the dura under direct vision. Plasma morphine concentrations measured by specific radioimmunoassay showed that peak concentrations occurred significantly earlier with heroin (4.7 +/- 0.6 min, mean +/- SEM) than with morphine (7.6 +/- 0.9 min) and that peak concentrations were significantly higher after heroin 5-10 min after extradural injection. The fraction of extradural heroin crossing the dura was estimated to be 55% of the fraction of morphine crossing the dura. Postoperative fentanyl requirements using demand analgesia were the same with extradural morphine as with extradural heroin (mean, 6.6 micrograms/hr). Clinically significant slowing of respiratory rate occurred only after extradural heroin (three patients).

Adult↗

Evidence for two distinct classes of murine B cell growth factors with activities in different functional assays.

Several previously described B cell growth factor (BCGF) activities from a number of mouse monoclonal T cell sources were compared in different functional assays. The results indicate that there are two distinct classes of BCGF defined by functional activity and source. BCGF I, whose prototype is (EL4)BCGF, synergized with anti-Ig in the proliferation of normal splenic B cells but had no activity when dextran sulfate (DXS), rather than anti-Ig, was used to costimulate the same source of B cells. BCGF I also failed to directly stimulate BCL1 tumor B cells. In contrast, BCGF II, whose prototype is (DL)BCGF, showed a reciprocal pattern of activity. BCGF II failed to synergize with anti-Ig-costimulated normal B cells to give good proliferative responses. Sources of BCGF II also directly stimulated (no anti-Ig or DXS added) B cells of the BCL1 tumor-carrying mice. These results suggest that the two BCGF may have activity on two subsets of B cells that respond differentially to induction with the two polyclonal B cell activators, anti-Ig and DXS. The possibilities that these different patterns of response occur in separate lineages of B cells and/or in B cells in different states of differentiation is discussed.

Animals↗

The effect of different types of intrauterine devices on the risk of pelvic inflammatory disease.

A case-control study of 155 women with pelvic inflammatory disease (PID) and 305 controls with other conditions was carried out. Compared with other methods of contraception, the age-adjusted relative risk estimate for use of any intrauterine device (IUD) within one month of admission was 8.6 (95% confidence limits, 5.3 and 13.8); for past use, the estimate was 1.6 (confidence limits, 0.6 and 4.0). The estimated risk was highest for users of the Dalkon Shield (11 cases and two controls), lowest for users of copper-containing devices (40 cases and 34 controls), and intermediate for users of other IUDs, principally the Lippes Loop (32 cases and 23 controls). Compared with copper-containing IUDs, the relative risk estimate for the Dalkon Shield was 6.5 (confidence limits, 1.5 and 27.5). When the Dalkon Shield and copper-containing IUDs were compared, in turn, with the Lippes Loop, the estimated risk was increased for the former and reduced for the latter, but the differences were not statistically significant. Nine of the 11 cases using the Dalkon Shield (82%) had severe PID (diagnosed at surgery or because of purulent cervical discharge), compared with 12 of 40 copper device users (30%) and nine of 32 Lippes Loop users (28%). The risk of PID did not seem to be related to duration of use of any of the three IUDs. This study confirms that IUD use increases the risk of PID. It also suggests that the Dalkon Shield is more harmful than other devices.

Adult↗

Factors affecting steroid and prostaglandin secretion by reproductive tissues of cycling and pregnant sows in vitro.

The secretion of prostaglandin E2 and prostaglandin F2 alpha by endometrial and corpus luteum tissues of cycling and pregnant sows in vitro has been measured and the effects of arachidonic acid and steroids on prostaglandin secretion studied. In general, both types of tissues in vitro exhibited a gradually increasing capacity to secrete prostaglandin as the cycle progressed, although the most significant increase was in prostaglandin F2 alpha secretion. An inverse relationship between prostaglandin and progesterone secretion was observed, and, while indomethacin inhibited prostaglandin secretion, it was without effect on progesterone. A much lower capacity for prostaglandin secretion was observed with tissues from pregnant sows. Prostaglandin secretion by endometrial and corpus luteum tissues of the cycle were stimulated by exogenous arachidonic acid which also stimulated prostaglandin E2 secretion in pregnant sows. This clearly suggests that phospholipase A2 activity which controls substrate availability is of considerable importance in pig reproductive tissues. The effects of steroid hormones on prostaglandin secretion by pig reproductive tissues was more complex although in general oestradiol appeared to be inhibitory while progesterone was stimulatory.

Animals↗

Human hybridomas from patients with malignant disease.

Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants.

Antibodies, Monoclonal↗

Prolonged exercise in highly trained female endurance runners.

The effects of prolonged exercise in a 21 degree C dry bulb and 15 degree C wet bulb environment at 65%-70% VO2max were examined in seven highly trained females. The subjects, aged 22-35 years, underwent an initial incremental treadmill test to exhaustion, with assessment of VO2max and related cardiorespiratory variables. One week later, under similar environmental conditions, subjects ran at approximately 65% VO2max for 80 min on a motor-driven treadmill. Approximately 10 ml of venous blood was withdrawn 10 min prior and immediately prior to the onset of prolonged exercise, and at 20, 40, 60, and 80 min, and 20 min post-exercise. Venous blood was analyzed for glucose, lactate, osmolality, Na+, K+, protein, and hemoglobin (Hb). Hematocrit was measured and changes in plasma volume calculated. VO2, VE, respiratory exchange ratio, and heart rate were recorded at 17, 37, and 77 min. The percent body fat estimated from skinfold thicknesses was 19 +/- 1%. The mean VO2max was 59.3 +/- 1.0 ml . kg-1 . min-1, with a mean max VE STPD and heart rate of 78.75 +/- 3.10 1 . min-1 and 175 +/- 4 beats . min-1, respectively. No significant changes occurred in VO2, VE, % VO2max, heart rate, venous lactate, plasma glucose, or plasma protein during the prolonged exercise. A significant decrease in respiratory exchange ratio was noted. Significant changes also occurred in hematocrit, Hb, Na+, K+, and osmolality. An interesting finding was the pre-exercise expansion of the plasma volume.

Adipose Tissue↗

Localisation of malignant glioma by a radiolabelled human monoclonal antibody.

Human monoclonal antibodies were produced by fusing intratumoral lymphocytes from patients with malignant gliomas with a human myeloma line. One antibody was selected for further study after screening for binding activity to glioma cell lines. The patient from whom it was derived developed recurrent glioma. 1 mg of antibody was purified, radiolabelled with 131I, and administered intravenously. The distribution of antibody was determined in the blood, CSF and tumour cyst fluid and compared with that of a control human monoclonal immunoglobulin. Antibody localisation in the tumour was observed and confirmed by external scintiscanning.

Adult↗

Composite vascularized free-rib and pleural transfer for laryngotracheal reconstruction.

A free rib pleura composite vascularized transfer for repair of a defect in the canine upper airway is discussed and the results are reported. The procedure was carried out on eight dogs. One dog died from the anesthetic. Three surgical failures resulted from technical difficulties: failure of the vascular anastomosis, torsion of the vascular pedicle, and hemorrhage. Four dogs successfully maintained their airways, although one demonstrated bone absorption. Bone scanning, angiography, tetracycline labeling, and light microscopy confirmed active osseous metabolism of the rib, providing further evidence of the adequacy of periosteal circulation in maintaining normal rib metabolism. Scanning electron microscopy and conventional light microscopy demonstrated the development of a healthy respiratory epithelium. Thus a free flap of rib and attached pleura will provide adequate skeletal support and a moist, functioning, epithelial lining in a single-stage operation for reconstruction of a laryngotracheal defect in the normal dog.

Animals↗