Pyoderma gangrenosum in a patient with colonic tuberculosis.
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Adult↗
Biomedical subjects
J W Shin
Publications and source records attributed to J W Shin.
[Evaluation of two kinds of enzyme linked immunosorbent assay method for clonorchiasis].
In this present study, adult worm antigen of Clonorchis sinensis and the indirect and double sandwich enzyme linked immunosorbent assay (ELISA) were used to detect the serum antibody of clonorchiasis patients from the endemic area of Miao-Li county. The results of indirect ELISA revealed a good correlation between the egg stool count and IgG strength of patients sera. Focusing on the different immunoglobulin classes in serum, the patient group has significantly higher level than that of control group, and the IgG level was distinguishly higher than the other Igs. The result also showed that the sensitivity and specificity on the detection of IgG for clonorchiasis patient were 83.23% and 100%, respectively. After praziquantel treatment, the antibody titer of patients decreased in accord with the time period, and the decrease rate of antibody titer in patients with moderate infection was more closely related to the time after treatment than those in the other degree infection groups. When double sandwich ELISA method was used to detect serum antibody to C. sinensis in patients with the different parasitic infection, it was shown that antibody levels in patients with clonorchiasis were significantly higher than those in the un-identical parasitic infection patients. Both of the sensitivity and specificity by using this method were 100%. Based on the results of the indirect and double sandwich ELISA methods, it is recommended that uses of the double sandwich ELISA incorporated with the indirect ELISA may give a sufficient results on the epidemiological study of human clonorchiasis. It may also be recommended that double sandwich ELISA method can be used for a mass survey and indirect ELISA method for further detection in the patients with clonorchiasis.
Animals↗
A preliminary study of clinical staging in clonorchiasis.
Antibody responses to infection with metacercaria of Clonorchis sinensis were studied in triplet cats. Elevated IgM antibody first appeared seven days after infection, followed by elevation of IgA and IgG. The peak titers of IgM and IgA antibodies were found at 3 weeks and 11 weeks, respectively. IgG antibody was elevated at 3 weeks and reached a peak, then a plateau at 13 weeks. Duration of antibody detection after infection was 1 week to 5 weeks for IgM, and 2 weeks to 28 weeks for IgA. IgM and IgA antibody titers decreased to normal level spontaneously without significant effect by the treatment. Using the animal model, the three clinical stages were classified according to the antibody patterns: i.e. acute stage, IgM(+) IgA(+) IgG(+); subacute stage, IgM(-) IgA(+) IgG(+); Chronic stage, IgM(-) IgA(-) IgG(+). Of 77 human patients with clonorchiasis, there were 3 (3.9%) patients who had subacute infection and 74 (96.1%) patients were in chronic stage.
Animals↗
[Humoral immune responses to stages of Brugia pahangi in experimentally infected rats].
In the present experiment, Sprague-Dawley rats were inoculated with Brugia pahangi, a lymphatic dwelling filarial parasite. Each rat received 100 third stage larvae (L3) of B. pahangi freshly harvested from Aedes togoi. Rats in the control group were injected with saline. After inoculation, tail vein blood was collected from each rat at 2-week intervals. The immune responses of the infected rats to B. pahangi were measured by the enzyme-linked immunosorbent assay (ELISA). Four types of antigen were prepared from different stages of B. pahangi. The antigen extracts were prepared from microfilaria, L3, and male and female adult worms by homogenization, eight times of freezing and thawing, and centrifugation at 11,000 X g for 45 min at 4 degrees C. Aliquots of supernatant extracts were stored at -20 degrees C. These antigens were adjusted to the same concentration of their protein contents and employed in the ELISA test. Serial serum dilutions were made to determine the concentrations of stage-specific antibodies in each serum sample collected during the infection course. Microfilaria specific antibodies appeared in the blood of infected rats at the 6th week of infection. Peak titer was observed at the 10th week. Subsequently, a gradual decrease was seen in the following month. Serum antibody titers against L3 and adult antigens rised since the 4th week and reached to the peak at 14th week. The exist of common antigens shared by the mature adults and infective larvae were detected by the ELISA and conformed by disc gel electrophoresis.
Animals↗
Use of the enzyme-linked immunosorbent assay (ELISA) for detection of circulating antibodies to human leishmaniasis.
In an attempt to lay the background work of immunodiagnosis of human leishmaniasis in Taiwan, the usefulness of enzyme-linked immunosorbent assay (ELISA) for detection serum antibodies in large regional groups was studied. Antigens were prepared from promastigotes of Leishmania tropica major recently isolated in our laboratory by Chao. One hundred and sixty-five serum samples were collected from medical students of National Yang-Ming Medical College (YM), Taipei, 61 from residents of San-hsing District (SH), I-lan County, 9 from residents of Wu-lai District (WL), Taipei County, 2 from patients with cutaneous leishmaniasis, 6 from laboratory workers in the Department of Parasitology (LW), National Yang-Ming Medical College, and 1 from a patient with the history of hypersensitive to mosquito bites. The result of the ELISA were analyzed statically. YM students had the lowest serum antibody levels to this parasite. The laboratory workers and the SH residents had a significantly higher antibody prevalence rate than the YM students, but a significantly lower rate than positive patients. Positive/pseudopositive rate in non-patient groups was 0.82%. Tracing of these two suspected cases are in progress. The regional high antibody levels in the SH residents suggest that a risk of human leishmaniasis may be significant in the population tested. Using our antigen preparations we demonstrated that the ELISA is sensitive, rapid, and easy to perform and is therefore useful in screening and detecting of circulating anti-leishmania antibodies in serosurvey of large sample size.
Analysis of Variance↗