Search PubMed⌕ Search

Biomedical subjects

J W Morris

Publications and source records attributed to J W Morris.

At least 19 recordsLinked to original sources

Phonon instabilities and the ideal strength of aluminum.

We have calculated the phonon spectra of aluminum as a function of strain using density functional perturbation theory for <110>, <100>, and <111> uniaxial tension, as well as relaxed <112>[111] shear. In all four cases, phonon instabilities occur at points away from the center of the Brillouin zone and intrude before the material becomes unstable according to elastic stability criteria. This is the first time the ideal strength of a metal has been shown to be dictated by instabilities in the acoustic phonon spectra. We go on to describe the crystallography of the unstable modes, all of which are shear in character. This work further suggests that shear failure is an inherent property of aluminum even in an initially dislocation-free perfect crystal.

Journal Article↗

Mechanical instability and ideal shear strength of transition metal carbides and nitrides.

The ideal shear strength of transition metal carbides and nitrides is calculated with the use of the ab initio pseudopotential density functional method. The microscopic mechanism that limits the ideal strength is studied using full atomic and structural relaxation and the results of electronic structure calculations. It is shown that plasticity in perfect crystals can be triggered by electronic instabilities at finite strains. Our study explicitly demonstrates that the ideal strength in these materials is limited by the elastic instability which is in turn initiated by electronic instabilities. The potential application of alloy hardening due to the onset of instabilities at different strains is also discussed.

Journal Article↗

Development of a Nanoindenter for In Situ Transmission Electron Microscopy.

In situ transmission electron microscopy is an established experimental technique that permits direct observation of the dynamics and mechanisms of dislocation motion and deformation behavior. In this article, we detail the development of a novel specimen goniometer that allows real-time observations of the mechanical response of materials to indentation loads. The technology of the scanning tunneling microscope is adopted to allow nanometer-scale positioning of a sharp, conductive diamond tip onto the edge of an electron-transparent sample. This allows application of loads to nanometer-scale material volumes coupled with simultaneous imaging of the material's response. The emphasis in this report is qualitative and technique oriented, with particular attention given to sample geometry and other technical requirements. Examples of the deformation of aluminum and titanium carbide as well as the fracture of silicon will be presented.

Journal Article↗

A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07.

Arabidopsis thaliana BAC T07M07 encoding the abscisic acid-insensitive 4 (ABI4) locus has been sequenced completely. It contains a 95,713-bp insert and 24 predicted genes. Most putative genes were confirmed by gel-based RNA profiling and a cluster of ABA-regulated genes was identified. One of the 24 genes, designated PP2C5, encodes a putative protein phosphatase 2C. The encoded protein was expressed in Escherichia coli, and its enzyme activity in vitro was confirmed.

Abscisic Acid↗

Construction of an approximately 2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2.

A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artificial chromosome (YAC) physical map is described. An approximately 2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.

Arabidopsis↗

Assessing probability of paternity and the product rule in DNA systems.

The genetic resolution of paternity disputes begins with an intricate detection of inherited traits and finishes with a statistical inference (the probability of paternity, W). Notwithstanding some initial fanfare, statistical inference is a necessary component of DNA-based paternity tests because band patterns may be rare but not yet unique, and even rare events in a vacuum are meaningless. The genetic match must be combined with other evidence for relevancy, thus a Bayesian approach is preferred when computing W. This paper reviews the standard model used to compute W and discusses the model's various properties and assumptions. The standard model is extended to include DNA systems in which alleles are operationally continuous due to measurement error. This extension avoids problems associated with 'matched/non-matched' binned decisions. After outlining the model assumptions for a single DNA system, particular attention is given to the product rule-the procedure of multiplying intermediate probabilities across genetic loci to form a combined W. An empirical alternative to the product rule is also assessed and correlated with standard procedures.

Alleles↗

A comparison of linear and exponential models for drug expiry estimation.

While a large portion of pharmaceutical stability data is known to follow an exponential model decay, linear modeling of this data for expiry estimation is the norm. Expiry predictions based on linear and exponential fits to stability data were made to estimate the bias due to the linear fitting. It was found that within the usual expiry limits on drug potency, the difference between the model fits is relatively trivial. In cases of loss of potency greater than 15%, small assay variability, or great disparity between the length of the study and the time of expiry prediction, however, there is a nontrivial difference in the predictions and the exponential model is preferable.

Drug Compounding↗

Rapid identification of cytokinins by an immunological method.

A method for rapid identification of bacterial cytokinins has been developed in which cultures are fed [(3)H]adenine, the cytokinins (including (3)H-labeled cytokinins) are isolated by immunoaffinity chromatography, and analyzed by HPLC with on-line scintillation counting. Analysis of Agrobacterium tumefaciens strains showed that some produced primarily trans-zeatin, whereas others produced primarily trans-zeatin riboside. Pseudomonas syringae pv savastanoi produced mixtures of trans-zeatin, dihydrozeatin, 1''-methyl-trans-zeatin riboside, and other unknown cytokinin-like substances. Corynebacterium fascians, produced cis-zeatin, isopentenyladenine and isopentenyladenosine. The technique is designed for qualitative rather than quantitative studies and allows ready identification of bacterial cytokinins. It may also have utility in the study of plant cytokinins if adequate incorporation of label into cytokinin precursor pools can be achieved.

Journal Article↗

KVP meter errors induced by plastic wrap.

The purpose of this study was to determine whether erroneous kVp meter readings, induced by plastic wrap, affected the actual kVp (output) of a dental X-ray machine. To evaluate the effect of plastic wrap on dental X-ray machine kVp meters, a radiation output device was used to measure output in mR/ma.s. An intraoral dental X-ray unit (S.S. White Model #90W) was used to make the exposures. First, the kVp meter was not covered with plastic wrap and output readings were recorded at various kVp settings with the milliamperage and time held constant. Secondly, the same kVp settings were selected before the plastic wrap was placed. Milliamperage and time were again held to the same constant. The X-ray console was then covered with plastic wrap prior to measuring the output for each kVp. The wrap possessed a static charge. This charge induced erroneous kVp meter readings. Out-put readings at the various induced kVp settings were then recorded. A kVp of 50 with no wrap present resulted in the same output as a kVp of 50 induced to read 40 or 60 kVp by the presence of wrap. Similar results were obtained at other kVp settings. This indicates that the plastic wrap influences only the kVp meter needle and not the actual kilovoltage of the X-ray machine. Dental X-ray machine operators should select kVp meter readings prior to placing plastic wrap and should not adjust initial settings if the meter is deflected later by the presence of wrap. The use of such a procedure will result in proper exposures, fewer retakes, and less patient radiation. If plastic wrap leads to consistent exposure errors, clinicians may wish to use a 0.5% sodium hypochlorite disinfectant as an alternative to the barrier technique.

Communicable Disease Control↗

Identification of an Agrobacterium tumefaciens virulence gene inducer from the pinaceous gymnosperm Pseudotsuga menziesii.

Inducible T-strand mobilization from the Ti plasmid of Agrobacterium tumefaciens to the genome of a plant host is mediated by the activation of a cascade of bacterial virulence genes. It is initiated when the bacterium senses the presence of a low molecular weight inducer secreted by the plant. Although many hydroxyphenylpropanoid and phenolic compounds can activate the virulence cascade, the only native inducers that have been identified to date are acetosyringone and hydroxyacetosyringone. A new inducer, the phenylpropanoid glucoside coniferin, has now been isolated from Pseudotsuga menziesii (Douglas-fir). Agrobacterium strains that were more tumorigenic on gymnosperms were more effectively induced by coniferin.

Cinnamates↗

Cytokinins in Vegetative and Reproductive Buds of Pseudotsuga menziesii.

Immunoaffinity techniques using columns of immobilized antibodies raised against zeatin riboside and isopentenyladenosine were found to be effective in isolating cytoklnins from vegetative, female, and male buds of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco). The purified cytokinins were separated by reverse phase high performance liquid chromatography and analyzed by radioimmunoassay. Confirmation of cytokinin identities was by gas chromatography-mass spectrometry. Immediately prior to bud burst, all bud types contained three major cytokinins: isopentenyladenosine, zeatin riboside, and a hexose conjugate of zeatin riboside (not zeatin riboside O-glucoside). Zeatin-type cytokinins were present in relatively high concentration in vegetative and female buds. In male buds, however, relatively high levels of isopentenyladenosine were found together with low levels of zeatin-type cytokinins.

Journal Article↗

The proto-oncogene c-fos is over-expressed in the majority of human osteosarcomas.

The expression of fos protein was examined in 30 cases of human osteosarcoma as formalin-fixed and paraffin-embedded tissue sections using two monoclonal antibodies and, for five cases, as frozen sections using 3 polyclonal antibodies. Nuclear antibody labeling intensities were determined either by visual scoring (3 pathologists) or by microdensitometry and included over 700 tumor, 350 normal and 150 benign tissue observations. Visual scores were shown to be linear with optical density with a correlation coefficient of 0.97. Analysis of the osteosarcoma cases at one antibody concentration revealed two groups: a minority (39%) with a low average visual score of 2 +/- 0.2 which was very similar to benign and normal tissues, and a majority (61%) with an average score of 3.1 +/- 0.3. The difference is highly significant (t-test), P less than or equal to 0.01. The results were supported by an analysis of partial immunotitration curves using a curve-fitting procedure which yielded estimates of FOSo (maximum relative fos protein concentration) which were ca. 150% increased for the majority tumor group compared to the minority group or benign or normal tissues. In previous studies of v-sis transformed cells, which exhibit ca. 300% over-expression of c-fos protein as observed here, antisense techniques were used to show that over-expression leads to increased growth and loss of contact inhibition. Thus the combined results suggest that steady state c-fos protein is significantly elevated in, and may contribute to, the aggressive growth properties of a majority of human osteosarcomas.

Antibodies, Monoclonal↗

Atmosphere contamination following repainting of a human hyperbaric chamber complex.

The Naval Medical Research Institute currently conducts hyperbaric research in a Man-Rated Chamber Complex (MRCC) originally installed in 1977. Significant engineering alterations to the MRCC and rusting of some of its interior sections necessitated repainting, which was completed in 1988. Great care was taken in selecting an appropriate paint (polyamide epoxy) and in ensuring correct application and curing procedures. Only very low levels of hydrocarbons were found in the MRCC atmosphere before initial pressurization after painting and curing. After pressurization, however, significant chemical contamination was found. The primary contaminants were aromatic hydrocarbons: xylenes (which were a major component of both the primer and topcoat paint) and ethyl benzene. The role that pressure played in stimulating off-gassing from the paint is not clear; the off-gassing rate was observed to be similar over a large range in chamber pressures from 1.6 to 31.0 atm abs. Scrubbing the chamber atmosphere with the chemical absorbent Purafil was effective in removing the contaminants. Contamination has been observed to slowly decline with chamber use and is expected to continue to improve with time. However, this contamination experience emphasizes the need for a high precision gas analysis program at any diving facility to ensure the safety of the breathing gas and chamber atmosphere.

Air Pollutants↗

Biostatistical evaluation of evidence from continuous allele frequency distribution deoxyribonucleic acid (DNA) probes in reference to disputed paternity and identity.

We present a development and discussion of the biostatistical evaluation of deoxyribonucleic acid (DNA) probe evidence in forensic science cases of disputed paternity and identity. We restrict ourselves to single-locus codominant systems (highly analogous to more conventional systems) which have the apparently novel complication of an experimentally continuous allele frequency distribution. This complication necessitates reformulations of standard biostatistical summaries of the evidence (the paternity index (PI) and the phenotype frequency, respectively). These reformulations, rather than representing a unique case, have applicability to the evaluation of evidence obtained in standard genetic systems now in widespread use.

DNA↗

PGM1 and Gc subtype gene frequencies in a California Hispanic population.

The gene frequencies of the subtypes of the Gc (group-specific component) protein and PGM1 (phosphoglucomutase) enzyme systems have been determined by means of isoelectric focusing for a California Hispanic population (no. = 404). The Gc subtyping, done by immunofixation on a polyacrylamide gel, gave the following results: 1s = 0.491; 1f = 0.288; 2 = 0.218; variant = 0.0025. The PGM subtyping, done on agarose gel, gave results as follows: 1+ = 0.502; 1- = 0.266; 2+ = 0.128; 2- = 0.104. Because the gene frequencies for Hispanics in both these systems are relatively evenly balanced, the systems are of great value in Hispanic paternity investigations. The average power of exclusion is calculated to be 34.4% (Gc subtyping) and 39.8% (PGM1 subtyping).

Alpha-Globulins↗

Effects of ethanol, acetaldehyde and acetate on insulin release from perifused pancreatic islets.

It has been postulated that ethanol-induced changes in carbohydrate hemeostasis are mediated in part by alterations in pancreatic endocrine function. In this study ethanol (40, 120 and 240 mg/dl) produced dose-related suppression of immunoreactive insulin (IRI) release from isolated rat islets perifused with glucose and theophylline. Acetaldehyde inhibited IRI release at a concentration (39 mg/dl) much higher than can be achieved in vivo. A high concentration (29 mg/dl) of acetate did not influence IRI release. These results suggest that the ability of ethanol to inhibit IRI release resides principally in the parent compound and not its metabolites.

Acetaldehyde↗

Effect of ethanol on basal and arginine-stimulated plasma levels of glucose, insulin and glucagon in fasted unanesthetized rabbits.

Plasma glucose, insulin (IRI) and glucagon (IRG) levels were determined in fasting unanesthetized rabbits following pretreatment of either saline or ethanol (1.5 g/kg p.o.) and i.v. infusion of either saline or arginine (10 mg/kg/min for 30 min). Ethanol lowered plasma glucose and IRI levels but raised IRG levels in rabbits infused with saline. Arginine-induced hyperglycemia and hyperinsulinemia were markedly suppressed by ethanol so that any further increase expected by the infusion of arginine was minimized. Thus, ethanol not only alters basal levels of IRI, IRG and glucose but also suppresses arginine-stimulated levels as well.

Animals↗