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Biomedical subjects

J W Lane

Publications and source records attributed to J W Lane.

34 records · Page 2Linked to original sources
PubMed1970

Ultrastructural changes during the yeastlike to mycelial-phase conversion of Blastomyces dermatitidis and Histoplasma capsulatum.

Fine details of the sequential anatomical events occurring during yeast to mold morphogenesis of the dimorphic fungal pathogens Blastomyces dermatitidis and Histoplasma capsulatum as seen in ultrathin sections are described and illustrated by electron micrographs. Discrete intracytoplasmic membrane systems intimately associated with the plasma membrane were observed to be formed within 6 to 8 hr after induction of the conversion process. Within 12 to 18 hr, an intermediate or transitional cell with Woronin bodies at the septum was formed from the converting yeastlike cell. Both cells were noted to contain increased numbers of mitochondria. At approximately 48 hr from the initial induction of the conversion stimuli, the newly forming hyphal cells were observed to produce postconversional intracytoplasmic membrane systems seen normally in the ultrastructural organization of the fully established mycelial-phase cell. These membrane systems appear to be associated with normal septal formation. Although minor variations of time were observed in the occurrence of the sequential events, it is suggested that yeastlike to mycelial-phase conversion of these two fungal pathogens proceeds via a similar mechanism of ultrastructural reorganization.

Blastomyces↗
PubMed1969

Ultrastructural studies on the yeastlike and mycelial phases of Sporotrichum schenckii.

Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods of fixation and embedding. The internal morphology of the two forms of yeastlike S. schenckii was in many ways similar to that of similarly dimorphic fungi and yeasts studied by other authors. However, the use of the glutaraldehyde-osmium in agar fixation technique suggests the presence of an electron transparent capsular or slime layer with associated electron dense microfibrils to be present external to the cell wall of the yeastlike phase but not the mycelial phase. Mycelial phase S. schenckii was found to contain many of the internal microstructures reported for other filamentous dimorphic fungi. Conidia production in agitated liquid culture was found to be restricted since only rare sessile conidia were observed.

Aldehydes↗
PubMedDate not supplied

Plexiform fibrohistiocytic tumor of the foot.

The first case of plexiform fibrohistiocytic tumor in the foot is presented in this article. The tumor developed on the dorsum of the left foot in a 14-year-old female. This tumor was originally described in 1988 by Enzinger and Zhang. Their study indicated that this tumor has a female predominance, median age of 14.5 years, 63% located in the upper extremities, 37.5% recurrence rate, and 3% metastasis rate. These tumors are very unique with a nodular pattern and a cellular component of histiocytes, fibroblasts, and multinucleated giant cells. Typically they are located within the deep dermis and subcutaneous tissue. Immunohistochemical preparations show that the tumor does not stain for S-100 protein, desmin, cytokeratin, factor VIII-related protein, or lysozyme. However, it does stain for alpha-1-antitrypsin, alpha-1-antichymotrypsin, alpha-smooth muscle-specific actin, vimentin, and CD68 antibody.

Adolescent↗
PubMedDate not supplied

Vertical cross contamination of trichloroethylene in a borehole in fractured sandstone.

Boreholes drilled through contaminated zones in fractured rock create the potential for vertical movement of contaminated ground water between fractures. The usual assumption is that purging eliminates cross contamination; however, the results of a field study conducted in a trichloroethylene (TCE) plume in fractured sandstone with a mean matrix porosity of 13% demonstrates that matrix-diffusion effects can be strong and persistent. A deep borehole was drilled to 110 m below ground surface (mbgs) near a shallow bedrock well containing high TCE concentrations. The borehole was cored continuously to collect closely spaced samples of rock for analysis of TCE concentrations. Geophysical logging and flowmetering were conducted in the open borehole, and a removable multilevel monitoring system was installed to provide hydraulic-head and ground water samples from discrete fracture zones. The borehole was later reamed to complete a well screened from 89 to 100 mbgs; persistent TCE concentrations at this depth ranged from 2100 to 33,000 microg/L. Rock-core analyses, combined with the other types of borehole information, show that nearly all of this deep contamination was due to the lingering effects of the downward flow of dissolved TCE from shallower depths during the few days of open-hole conditions that existed prior to installation of the multilevel system. This study demonstrates that transfer of contaminant mass to the matrix by diffusion can cause severe cross contamination effects in sedimentary rocks, but these effects generally are not identified from information normally obtained in fractured-rock investigations, resulting in potential misinterpretation of site conditions.

California↗