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J W Kelly

Publications and source records attributed to J W Kelly.

113 records · Page 7Linked to original sources
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HPLC separation of pentazocine enantiomers in serum using an ovomucoid chiral stationary phase.

A stereospecific HPLC method was developed for the analysis of (-) and (+) pentazocine in human serum. Each enantiomer and the internal standard nalophine were isolated from serum using a liquid-liquid extraction procedure. Recoveries of 99.05 +/- 5.37 and 97.42 +/- 2.78% were obtained for (-) and (+) pentazocine, respectively. Resolution of the enantiomers was obtained by using an ovomucoid chiral stationary phase with a mobile phase of methanol:acetonitrile: 10 mM phosphate buffer, pH 5.8 (20:5.3:74.7 v/v/v). A resolution (Rs) value of 1.80 was obtained for the pentazocine enantiomers. Linear calibration curves were obtained in the 10-100 ng/mL range for each enantiomer in serum. The detection limit based on a signal-to-noise ratio of 3 was 5 ng/mL for each enantiomer in serum using fluorescence detection with excitation at 275 nm and emission set at 335 nm. The lowest quantifiable level was found to be 10 ng for each enantiomer. Precision and accuracy of the method were in the 3.8-4.8% and 1.3-4.2% ranges, respectively.

Chromatography, High Pressure Liquid↗
PubMedDate not supplied

Liquid chromatographic separation of praziquantel enantiomers in serum using a cellulose-based chiral stationary phase.

R(-) and S(+) praziquantel (PZQ) in human serum were resolved and quantified using a stereospecific LC method. Each enantiomer and the internal standard 2-methylamino-5-chlorobenzophenone were isolated from serum using a solid-phase extraction procedure on a cyanopropyl column. Recoveries of 98.8, 93.3 and 35.4% were obtained for R(-) PZQ, S(+) PZQ, and the internal standard, respectively. A cellulose-based chiral analytical column (Chiralcel OD) was used with a mobile phase consisting of hexane-2-propanol (70:30, v/v). Linear calibration curves were obtained in the concentration range 50-1000 ng ml-1 for each enantiomer in serum. The detection limit for each enantiomer in serum using UV detection at 212 nm was 5 ng ml-1 (S/N = 3). The limit of quantitation of each enantiomer was 25 ng ml-1.

Cellulose↗
PubMedDate not supplied

Provider resource planning. A study of methods in multispecialty groups and systems.

This article profiles the state of the art in provider resource planning based on a sample of large multispeciality group practices. Senior administrative representatives from a geographically dispersed sample of 12 practices, each employing over 100 physicians, participated in telephone interviews to discuss their respective organization's provider resource planning practices. There was significant variation with regard to the structural and process components and evaluation criteria used to plan provider resources. Variability was most pronounced by the lack of consensus and experience within and across organizations regarding the number and type of criteria deemed useful in evaluating new position requests. This research suggests that there is an opportunity to develop more objective provider resource planning methods that recognize differences across organizations and their respective markets. The authors recommend a series of research steps to develop methods that would identify needs prospectively, minimize the complexities of provider resource planning and maximize the alignment of strategic plans and operations.

Benchmarking↗