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J W Jacobson

Publications and source records attributed to J W Jacobson.

At least 19 recordsLinked to original sources
PubMed1995

Identification, cloning, and sequencing of DNA essential for encapsulation of Streptococcus pneumoniae.

This paper reports the cloning and sequencing of a region of DNA from Streptococcus pneumoniae serotype 3 surrounding transposon Tn916, insertion of which was previously shown to result in lack of expression of the extracellular capsule. Sequence analysis revealed that the transposon inserted into a consensus insertion site 71 bp from the 5' end of the cloned fragment. Within the clone, 3' downstream regions from two different pneumococcal lytA genes were identified, as well as a putative 194 AA open reading frame (ORF1). Moreover, two copies of the repeat element BOX, oriented in opposite directions, were located immediately 3' of orf1. Within the region bounded by the first pair of internal sequencing primers, analysis revealed that the fragment amplified by PCR was always of the same size. Moreover, Southern blotting showed that for all serotypes examined to date, homology exists with the cloned fragment. These results indicate that this region of the chromosome is highly conserved and, taken together with other independently derived data, suggest that interruptions or deletions within this DNA lead to unencapsulation.

Bacterial Capsules↗
PubMed1994

The source of messages produced during facilitated communication with a boy with autism and severe mental retardation: a case study.

Assessed the source of messages produced through facilitated communication with a youth with autism and severe mental retardation using message-passing and picture-naming procedures. Results indicated that the facilitator was the source of the communications produced during the assessment; correct responding occurred only when the facilitator had knowledge of the correct answer. These findings are consistent with the results of other research demonstrating cuing, influence, or control of facilitated communications by the individuals providing assistance (facilitators). Implications for ethical practices by professionals in the context of facilitated communications are briefly discussed.

Autistic Disorder↗
PubMed1993

Evolution of the chordate muscle actin gene.

The ascidians Styela plicata, S. clava, and Mogula citrina are urochordates. The larvae of urochordates are considered to morphologically resemble the ancestral vertebrate. We asked whether larval and adult ascidian muscle actin sequences are nonmusclelike as in lower invertebrates, musclelike as in vertebrates, or possess characteristics of both. Nonmuscle and muscle actin cDNA clones from S. plicata were sequenced. Based on 27 diagnostic amino acids, which distinguish vertebrate muscle actin from other actins, we found that the deduced protein sequences of ascidian muscle actins exhibit similarities to both invertebrate and vertebrate muscle actins. A comparison to muscle actins from different vertebrate and invertebrate phylogenetic groups suggested that the urochordate muscle actins represent a transition from a nonmusclelike sequence to a vertebrate musclelike sequence. The ascidian adult muscle actin is more similar to skeletal actin and the larval muscle actin is more similar to cardiac actin, which indicates that the divergence of the skeletal and cardiac isoforms occurred before the emergence of urochordates. The muscle actin gene may be a powerful probe for investigating the chordate lineage.

Actins↗
PubMed1993

A brief history of the pneumococcus in biomedical research: a panoply of scientific discovery.

Because of its prominence as a cause of disease in humans, Streptococcus pneumoniae has been the subject of intensive investigation at both the clinical level and the basic scientific level during the past century. In a number of instances, these studies have resulted in important progress toward the comprehension of basic biological principles. The areas advanced by studies of the pneumococcus include an understanding of the concept of pathogenesis of infectious disease; the development of Gram's stain for identification of bacteria in specimens from patients; the elucidation of the role of the bacterial capsule in resistance to phagocytosis by cells of the host's immune system; the demonstration that molecules other than proteins are capable of eliciting the host's humoral immune responses and later, by extension, that isolated bacterial exopolysaccharides can be used safely and effectively as vaccines in humans; the documentation of the efficacy of penicillin; the collection of conclusive evidence that DNA encodes genetic information; and the investigation of putative proteinaceous virulence factors.

Animals↗
PubMed1993

The association between psychiatric diagnoses and severe behavior problems in mental retardation.

To investigate the relationship between psychiatric disorders and severe behavior problems in mental retardation, statewide client databases from developmental disabilities services in California (N = 89,419) and New York (N = 45,683) were analyzed and juxtaposed. The study focussed on nine major DSM-III-R psychiatric categories (or their equivalents), and severe forms of aggressive behavior, property destruction, self-injurious behavior, and stereotyped behavior in individuals 45 years old and younger with mental retardation of all levels of severity. In California, 3.9% had at least one psychiatric diagnosis; in New York, 5.4%. The rate of specific psychiatric diagnoses was variable across states, suggesting local preferences in diagnostic practices. Severe behavior problems occurred in 22.1% in California and in 41.4% in New York. This difference in rates can be attributed in part to different recording criteria for behavior problems. With regard to the association between psychiatric diagnoses and problem behaviors the results were consistent across databases: No compelling correlations were found. This means that neither aggression, self-injury, destruction, nor stereotypies determine whether a person receives a psychiatric diagnosis or not.

Adolescent↗
PubMed1993

An experimental assessment of facilitated communication.

This report presents a quantitative study of facilitated communication. Participants were 12 people living at an institutional autism program and 9 people who provided them with facilitated communication support. These subjects were the 12 most competent producers of facilitated communication in the program. They were shown pictures of familiar objects and asked to type the names of the objects under three conditions: (a) assisted typing with facilitators unaware of the content of the stimulus picture, (b) unassisted typing, and (c) a condition in which the participants and facilitators were each shown pictures at the same time. In this last condition the paired pictures were either the same or different, and the participant's typing was facilitated to label or describe the picture. These participants were unable to succeed in the tasks without facilitator assistance. On trials when the facilitators and participants had different pictures, the only "correct" labels were for pictures shown to the facilitators and not shown to the participants. This finding demonstrates that the facilitators were unknowingly determining what was typed.

Adolescent↗
PubMed1992

Review of Psychology on a Disk from CMS academic software: interactive activities for psychology.

Psychology on a Disk contains a variety of simulations that show a strong potential to motivate and educate introductory psychology students with a wide range of ability and initial interest. The exercises appear to be well within the grasp of first-year students and provide suitable starting points for discussion of terms, definitions, and areas of research activity. The quality of the simulations is high and provides some indication that there are additional areas of undergraduate psychology, for example, courses in abnormal, clinical, and developmental topics, in which specialized simulations might also constitute an important adjunct to classroom and laboratory activities. The 10 simulations span much of the content of introductory courses, although they are most pertinent to syllabi that emphasize learning principles and social psychology as important foundations. As an instructional device, the simulations also will serve to introduce some students to use of computers as a source of information and feedback with potential benefits in other and later academic endeavors in which time-savings will be important. Promotional materials describe POAD as easy to add (to existing texts), easy to use, easy to grade, easy to afford, and easy to order (through your college bookstore): truth in advertising.

Computer Simulation↗
PubMed1992

Compliance and quality in residential life. Limitations of regulations as a means of social reform in developmental services.

It has become obvious that community care systems for people with developmental disabilities are not fully realizing some of the stated goals toward which they are directed, despite great process. The foundations and processes by which state agencies regulate and whether the regulatory process can be directed to issues affecting the quality of life of people receiving developmental services were explored. Limitations and side effects of regulations were discussed with respect to the unpredictability of long-term effects, problems in measuring compliance, economic rather than social focus, the changing state of knowledge, and special considerations associated with the field of developmental services.

Activities of Daily Living↗
PubMed1991

Comparison of alcohol dehydrogenase expression in Drosophila melanogaster and D. simulans.

Alcohol dehydrogenase (ADH) gene expression was analyzed in Drosophila melanogaster and its sibling species D. simulans. The levels of ADH activity, ADH-cross-reacting material (CRM), and ADH-mRNA were analyzed for several strains of each species, which derive from diverse geographic locations around the world. There is considerable quantitative variation in ADH activity, CRM level, and RNA level among strains within species at all developmental stages. However, the only consistent differences between the two species are in pupal RNA level and in late-adult activity and CRM level. Late-adult melanogaster flies that are homozygous for the Slow allozyme have approximately twice the level of ADH activity and CRM as do simulans flies. The regression of activity on CRM over strains is highly significant and essentially the same for each species, which means that most, if not all, of the activity difference between the species is due to a difference in concentration of the ADH protein. In contrast, there is no significant regression of CRM level on mRNA level in adults of either species; nor is there a significant difference in RNA level between species. Therefore, the difference in ADH protein concentration is not due to RNA template availability. Thus, the interspecific difference in ADH level in adults must be due either to a difference in the rate of translation of the two RNAs or to a difference in protein stability.

Alcohol Dehydrogenase↗
PubMed1990

Differences in adaptive functioning among people with autism or mental retardation.

This report describes differences in motoric and instrumental activity of daily living skills (MADLs and IADLs) between 1,442 people with autism and 24,048 people with mental retardation, using data from an adaptive behavior measure. Comparisons were made using groups defined by age (5-12, 13-21, and 21-35 years) and intellectual level. Diagnoses of record were confirmed through group analyses of rates of problem behaviors consistent with autism and comparison to an independent data base. Findings suggest that at ages 5-12 the skills of children with autism are more developed than those of children with mental retardation matched by age and intellectual level. However, in the older groups these differences diminish, and with increasing age (21-35 years) more developed instrumental skills are observed for people with mental retardation.

Activities of Daily Living↗
PubMed1990

Mariner, Mos and associated aberrant traits in Drosophila mauritiana.

A suite of aberrant genetic traits, including increased mutation rate, sex-limited mutation and distorted transmission ratios, was produced among progeny of genetic crosses between two strains of Drosophila mauritiana when a paternally contributed Mos excision factor is placed into a non-Mos genetic background. In the reciprocal cross, involving maternally contributed Mos and Mos associated cytoplasm, the same genetic abnormalities are not observed. Differential effects on mariner excision in germ-line versus somatic tissue are apparent. Because Mos is known to influence the mobility of the mariner transposable element, these traits may be associated with mariner excision and/or transposition.

Animals↗
PubMed1990

Genetic basis of the difference in alcohol dehydrogenase expression between Drosophila melanogaster and Drosophila simulans.

Drosophila melanogaster and its sibling species, Drosophila simulans, differ in expression of the enzyme alcohol dehydrogenase (ADH). Adult melanogaster flies that are homozygous for the Slow allozyme have approximately twice the level of ADH activity and crossreacting material as simulans adults. There is no corresponding difference in ADH mRNA, however, so this difference in ADH protein level is evidently due to a difference in the rate of translation of the two RNAs and/or to a difference in protein stability. Here we report an interspecific gene-transfer experiment, using P-element transformation, to determine whether this expression difference is due to genetic background differences between the species (trans-acting modifiers) or to cis-acting factors within the Adh gene. When the Adh genes from D. melanogaster and D. simulans are put into the same genetic background, there is no detectable difference in their level of expression. The level is relatively high in the melanogaster background and relatively low in the simulans background. Therefore, the interspecific difference in Adh expression is due entirely to trans-acting modifiers, in spite of the many sequence differences between the Adh genes of the two species, which include two amino acid substitutions.

Alcohol Dehydrogenase↗
PubMed1990

Do some mental disorders occur less frequently among persons with mental retardation?

Distributions of psychiatric classifications among 42,479 persons with mental retardation were reviewed to determine whether relative rates of disorders for persons with both mental retardation and a mental disorder resembled those identified in other studies. Analyses conducted separately for children, adults, by sex, and by intellectual level indicated that rates of reported schizophrenic and psychotic disorders were generally higher than expected relative to rates of personality disorders, affective disorders, anxiety and phobic disorders, and behavior disorders. Factors that may have affected relative rates of identification were noted, and the need to develop more adequate assessment methodologies and conduct valid studies of population samples was underscored.

Adolescent↗
PubMed1990

Regulations: can they control staff compliance in human services systems?

Contemporary regulatory practices, in isolation, appear to potentially support mediocrity in program practices unless complemented by management practices that promote more exemplary personnel performance. Restatement of regulatory and supervisory practice from the standpoint of stimulus control procedures was offered as a vantage with utility for the improvement of administrative and supervisory practice. Generalization strategies were presented to promote effective compliance practices. The need for studies of the impact of regulatory changes upon personnel and the people they serve was stressed.

Behavior Therapy↗
PubMed1989

Psychological services for persons with mental retardation and psychiatric impairments.

Key informant characterizations of services provided by psychologists to persons with mental retardation and psychiatric impairments were described. Responses indicate that informants relied most heavily upon clinical history, observation and behavior checklists, and clinical signs in rendering diagnostic judgments. Therapeutic efforts were directed to a diversity of aspects of interpersonal and social adjustment, with benefit considered to be greater for persons with mild or moderate mental retardation.

Humans↗
PubMed1988

Single-chain antigen-binding proteins.

Single-chain antigen-binding proteins are novel recombinant polypeptides, composed of an antibody variable light-chain amino acid sequence (VL) tethered to a variable heavy-chain sequence (VH) by a designed peptide that links the carboxyl terminus of the VL sequence to the amino terminus of the VH sequence. These proteins have the same specificities and affinities for their antigens as the monoclonal antibodies whose VL and VH sequences were used to construct the recombinant genes that were expressed in Escherichia coli. Three of these proteins, one derived from the sequence for a monoclonal antibody to growth hormone and two derived from the sequences of two different monoclonal antibodies to fluorescein, were designed, constructed, synthesized, purified, and assayed. These proteins are expected to have significant advantages over monoclonal antibodies in a number of applications.

Amino Acid Sequence↗
PubMed1988

Problem behavior and psychiatric impairment within a developmentally disabled population. III: Psychotropic medication.

This report is the third in a series on problem behavior and psychiatric impairment in a population of 35,000 individuals receiving developmental disabilities services. Young and middle-aged adults were found to receive psychotropic medication at higher rates than children, adolescents, or elderly persons. Psychotropic receipt rates were found to increase with increasing severity of mental retardation, but most evidently with increasing restrictiveness of residential setting, increasing rated severity of problem behaviors, and presence of a psychiatric impairment. Rates of medication receipt also varied as a function of psychiatric diagnostic category. Discussion remarks emphasize the need to include information relative to clinical and social aspects of program settings and the roles and decision-making performance of physicians and psychologists in research on settings serving persons with developmental disabilities.

Adolescent↗