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Biomedical subjects

J W Freeman

Publications and source records attributed to J W Freeman.

At least 127 records · Page 7Linked to original sources
PubMed1985

Masking of nontumorous antigens for development of human tumor nucleolar antibodies with improved specificity.

Nucleoli purified from HeLa cells were spread by homogenization in low ionic strength buffer and by chelation of divalent cations. The antigenic determinants of these tumor nucleoli that are shared by normal liver nucleoli were masked by addition of rabbit anti-liver nucleolar antisera to form immune complexes. New Zealand White rabbits were immunized with these antibody-nucleolar complexes. The resulting antisera produced nucleolar fluorescence in HeLa cells but not in normal human liver or human kidney cells. One- and two-dimensional immunoblots identified a major Mr 58,000/pl 5.8 antigen in HeLa cells, which was not detected on corresponding immunoblots of normal human liver nucleoli. This method offers an improved approach to selection of tumor-associated antigens.

Animals↗
PubMed1983

Immunological heterogeneity of carcinoembryonic antigen: purification from meconium of an antigen related to carcinoembryonic antigen.

Two antigens cross-reactive with carcinoembryonic antigen (CEA) and distinct from the nonspecific cross-reacting antigen were identified in meconium by double immunodiffusion with a conventional goat anti-CEA antiserum. These two antigens together competitively inhibited cross-reacting antibodies against them in CEA radioimmunoassay and contributed to the measurement of meconium CEA levels which averaged 6 times higher than that determined with anti-CEA specific antibody. A purification method for one of these antigens, tentatively designated meconium antigen, is described and uses a combination of ethanol fractionation, ion-exchange and molecular sieve chromatography, and adsorption to an immunoadsorbent containing a cross-reactive murine monoclonal antibody to CEA. Preliminary characterization of the purified meconium antigen showed it to be a glycoprotein, migrating as an alpha-globulin and having a molecular size similar to that of CEA (Mr 185,000 versus 200,000). Antigenically, it lacks at least one determinant present on CEA and differs further from CEA by being weakly reactive with concanavalin A and resistant to proteolytic digestion with Pronase E. Although these properties of meconium antigen suggest that it may be nonspecific cross-reacting antigen 2, additional chemical and antigenic studies are required to establish its relationship to CEA and other CEA-related antigens in meconium.

Adenocarcinoma↗
PubMed1981

Laboratory evaluation of the AutoMicrobic system for identification of Enterobacteriaceae.

The Enterobacteriaceae Biochemical Card used with the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) was compared with the API 20E system (Analytab Products, Plainview, N.Y.) for identifying members of the family Enterobacteriaceae. A total of 1,401 clinical isolates representing 18 species were identified by the AutoMicrobic and API 20E systems over a 5-month period. Discrepancies between these systems were resolved by conventional methods. Overall, 98.3% of the isolates were identified correctly by the AutoMicrobic system in 8 h, with 94.2% having an AutoMicrobic system confidence level greater than or equal to 90%; 92.9% of the isolates were identified correctly by the 20E system after 24 h of incubation. Discrepancies between the two systems occurred in 3.1% of the isolates. Of these, 40.9 and 59.1% proved to be correct by the AutoMicrobic and API 20E systems, respectively.

Bacteriological Techniques↗
PubMed1981

The use of topical thrombin to reduce wound haematoma in patients receiving low-dose heparin.

The efficacy of topical thrombin in prevention of wound haematoma in patients receiving subcutaneous low-dose heparin was studied in a randomized, controlled trial. One hundred and two patients undergoing elective abdominal surgery were included in the trial. Haematological studies were carried out in the last 42 patients to determine whether introduction of thrombin into the wound resulted in any systemic effect via the coagulation pathways. The frequency of post-operative wound haematoma was significantly lower in the thrombin-treated group as compared to the control group and its action appeared to be independent of any change in coagulation parameters.

Abdomen↗
PubMed1980

Craniopharyngioma simulating bilateral internal ophthalmoplegia.

A 15-year-old girl had rapid onset of an apparent bilateral internal ophthalmoplegia. Subsequent evaluation revealed a large craniopharyngioma. It is uncommon for a mass to cause such eye findings and unique for a craniopharyngioma to manifest in this fashion.

Accommodation, Ocular↗
PubMed1976

Triage in civilian practice.

A triage area for the assessment and treatment of acutely ill patients on their admission to hospital was created. Four groups of patients were defined who were to be sent to this area rather than be treated in the casualty department. The area was contiguous with the hospital intensive care unit rather than the casualty department as the ease of providing trained staff members and equipment on a 24-hour basis outweighed the disadvantage of geographical separation from the casualty department. The increase in workload created for the intensive care members of staff was small, while the reduction in delay for seriously ill patients was considerable.

Acute Disease↗
PubMed1975

Oxprenolol and hydrallazine in the treatment of hypertension.

Oxprenolol and hydrallazine in combination were found to be as effective as methyldopa in a trial in hypertensive patients. The drugs could be given in a twice-daily dosage and had a much higher degree of patient acceptance. The side effects of this drug combination were usually due to hydrallazine and were mild and short-lived, but did require the withdrawal of one patient from the trial. Hydrallazine should be used with caution in the treatment of patients with a history of myocardial lschaemia.

Adult↗