Search PubMed⌕ Search

Biomedical subjects

J W Foster

Publications and source records attributed to J W Foster.

At least 109 records · Page 6Linked to original sources
PubMed1977

Characterization of an immunogenic fraction of Pasteurella multocida culture filtrates.

An immunogenic fraction (IF) of Pasteurella multocida strain P-1059 was separated from culture filtrate by Sephadex gel filtration. Additional fractionation of IF with aqueous ether resulted in the glycoprotein-like preparation (GLP) while extraction with aqueous phenol provided the lipopolysaccharide-like preparation (LPP). The unextracted IF contained carbohydrate, protein, and lipid; the GLP contained carbohydrate and protein; and the LPP contained carbohydrate and lipid. The GLP was maximally protective for mice against homologous challenge, and was medially toxic in rabbit skin when compared to the other culture-filtrate preparations; the LPP was maximally toxic in rabbit skin, and was least protective for mice; and the unextracted IF was medially protective for mice, and was least toxic in rabbit skin.

Animals↗
PubMed1975

Serologic studies of Brucella suis and Brucella canis.

Brucella suis strain 3b and Brucella canis strain RM-6-66 were grown on tryptose agar, and aqueous ether extracts were prepared from the cells. The aqueous phase was clarified by centrifugation for a total of 10 hours at 144,700 times g and fractionated with gels and with diethylaminoethyl (DEAE) cellulose. Two fractions from B suis and 1 fraction from B canis were reactive in the indirect hemagglutination test. The sensitizing ability was destroyed by pronase, but was unaffected by ribonuclease or deoxyribonuclease. Apparently, protein or one of its subunits has an important role in antigenic specificity relating to indirect hemagglutination activity. Results in immunoelectrophoretic analysis indicated at least 22 antigens were in the ultracentrifugal supernate (144,700 times g) of B suis and 31 antigens were in a similar supernate of B canis. Results in immunodiffusion and agglutination absorption studies indicated a close relationship existed between B suis and B canis.

Animals↗
PubMed1970

Fowl cholera immunization in turkeys. I. Efficacy of various cell fractions of Pasteurella multocida as vaccines.

Cell fractions of Pastuerella multocida (P-1059) were tested as vaccines against fowl cholera in turkeys. These fractions were culture filtrate, cell wall, and cytoplasm. A second culture filtrate preparation made from cells grown on blood-agar rather than the standard medium was also tested along with a "combination" preparation made by recombination of the cell fractions. Each preparation was tested in three vehicles: saline, alum (0.5%), and Freund Incomplete Adjuvant (50%). The turkeys vaccinated with these preparations were challenged by exposure to an experimental epornitic of fowl cholera. The combination fraction appeared to be the most promising vaccine when compared to the protective action of the commercial bacterin included in the test as a positive control.

Agar↗
PubMed1970

Fowl cholera immunization in turkeys. II. Use of experimental epornitic method to study vaccine efficacy in flocks of turkeys.

Groups of turkeys were challenged with Pasteurella multocida (P-1059) by the contact method. In this method, turkeys are artificially infected by the intramuscular injection of P. multocida organisms and are then introduced into the test group. The death patterns resulting from this contact method of challenge are either normally distributed or skewed to the right.

Animals↗
PubMed1970

Fowl cholera immunization in turkeys. 3. Significance of market quality in the evaluation of fowl cholera vaccines.

Turkeys vaccinated with various experimental vaccines and a commerical bacterin for fowl cholera and surviving an artificially induced epornitic were killed, and their carcasses were examined for wholesomeness. It was evident that, if the "fitness for human consumption" judgement was considered in addition to mortality, efficacy ratings of the various vaccines changed. This suggests that the "fitness for human consumption" factor be considered in future evaluation of biologicals for use in meat-producing birds.

Agar↗
PubMed1970

Immunization of mice with components of Pasteurella multocida.

Log-phase cells of Pasteurella multocida strain P-1059 were used to prepare isolated culture filtrate, cell wall, and cytoplasmic components. Culture filtrate was further separated by column chromatography. A portion of cytoplasm and culture filtrate was conjugated to ferritin by means of metaxylylene diisocyanate. Cell walls induced more protection in mice than the conjugated or unconjugated cytoplasm or culture filtrate. The cell walls caused edema and erythema when given intradermally in rabbits, whereas cytoplasm and culture filtrate produced dermal necrosis. The first of four chromatographically separated fractions of culture filtrate was possibly more immunogenic in mice than cell walls. This fraction was less reactive intradermally in rabbits than cell walls but more reactive than the other fractions.

Acrylates↗
PubMed1970

Comparison of Chemical Components of Cell Walls of Brucella abortus Strains of Low and High Virulence.

Amino acid, carbohydrate, and lipid components of cell walls of Brucella abortus strain 19A (low virulence) and strain 2308 (high virulence) were compared by thin layer chromatography (TLC) and by use of an amino acid analyzer. A total of 15 amino acids were detected by both chromatographic methods. Each amino acid was present in greater amounts in strain 2308 than in strain 19A when equal amounts of hydrolysates of cell wall and endotoxin-containing preparations were analyzed. A component with the same R(F) value as ethanolamine was present in strain 2308 cell wall hydrolysates but was not revealed by TLC of strain 19A cell wall hydrolysates. This component was not detected with the amino acid analyzer. TLC of cell walls tagged with 2,4-dinitrofluorobenzene prior to hydrolysis showed that phenylalanine was a terminal amino acid in cell walls of B. abortus strains 19A and 2308, B. suis strain 1776, and B. melitensis strain 2500. Carbohydrates detected in cell walls of strains 19A and 2308 by TLC were tentatively identified as glucose, mannose, rhamnose, and galactose. Colorimetric tests were also positive for 2-keto-3-deoxy-octulosonic acid, heptose, and dideoxyhexose. At least seven lipid components were detected by TLC of ether extracts of cell walls of strains 19A and 2308. It is suggested that one or more lipids is important in maintaining cell wall structure, because isolated cell walls rapidly became fragmented after exposure to ether.

Journal Article↗