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Biomedical subjects

J W Adams

Publications and source records attributed to J W Adams.

51 records · Page 3Linked to original sources
PubMed1980

A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene.

An unusually long repeated DNA sequence was identified in cloned DNA, three kb 3' to the human beta-globin gene. Other members of this repeated sequence family were isolated from a human genomic DNA library and characterized by Southern blotting techniques, electron microscopy, and solution hybridization. The copy located next to the beta-globin gene was found to be 6.4 +/- 0.2 kb long and continuous over that length. This repeated sequence family comprises about 1% of the human genome and contains 3000-4800 copies of moderate sequence divergence which are interspersed with other less-highly repeated DNA. The 6.4 kb repeated unit does not appear to be composed of any smaller tandemly repeated subunits, nor is it expressed at a high level in bone marrow cell RNA.

Bacteriophage lambda↗
PubMed1980

Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.

The human gamma-, delta-, and beta-globin genes are located within a 30-kilobase (kb) region of DNA, of which only 20% represents the globin genes. We have attempted to define the nature of flanking and intergenic sequences by isolating recombinants containing the human epsilon, both gamma-, or the 3' end of the beta-globin gene from a bacteriophage library of cloned human DNA. Comparison of these recombinants and a recombinant containing the delta- and beta-globin genes (H beta G1) has provided the following results. The epsilon-globin gene is located 14 kb 5' to the G gamma gene. DNA sequence homology between the region containing the two G gamma genes and the delta nd beta gene region is limited to only a few hundred nucleotides which include the globin coding sequences. Repetitive DNA sequences have been found in the region 3' to the beta-globin gene. Sequences located adjacent to the beta-globin gene are repeated in the globin gene region. A repetitive DNA sequence more than 3.2 kb long is repeated frequently in the human genome but is not repeated in the globin gene region in the clones examined.

Bacteriophage lambda↗
PubMed1978

Synthesis of DNA and poly(adenosine diphosphate ribose) in normal and chronic lymphocytic leukemia lymphocytes.

Peripheral blood lymphocytes were isolated from 9 patients with chronic lymphocytic leukemia (CLL) and 12 normal control donors. The cells were assayed for synthesis of DNA and poly-(adenosine diphosphate ribose) (poly[ADPR]) immediately after isolation and on successive days following their treatment with phytohemagglutinin (PHA). Two different techniques were used to measure DNA synthesis. In the standard technique, DNA synthesis was measured by incubating intact cells with [(3)H]deoxythymidine. In the new technique, the lymphocytes were first rendered permeable to nucleotides, then DNA synthesis was measured by incubating them with [(3)H]deoxythymidine triphosphate in the presence of deoxyATP, deoxyGTP, deoxyCTP, ATP, and Mg(++). Both assays showed the anticipated rise in DNA synthesis after PHA stimulation of normal cells. PHA-stimulated lymphocytes from patients with CLL demonstrated low levels of DNA synthesis in both assay systems. The initial levels of poly(ADPR) synthesis were greater in CLL lymphocytes than in normal cells. Studies with a T-cell-depleted population of normal cells showed the same activity for poly(ADPR) synthesis that was demonstrated by the original population of normal cells. PHA stimulation produced an increase in poly(ADPR) synthesis in both the normal and CLL cells. The increase in poly(ADPR) synthesis in normal cells was coincident with the increase in DNA synthesis. The increase in poly(ADPR) synthesis in the CLL cells was dissociated from the delayed and diminished increase in DNA synthesis. Thus, CLL cells have higher than normal initial levels of poly(ADPR) synthesis. Poly(ADPR) synthesis is dissociated from DNA synthesis in CLL cells whereas it varies directly with DNA synthesis in normal lymphocytes.

Cells, Cultured↗
PubMed1978

Financial awareness for the veterinarian in private practice.

The need for economic awareness is stressed, and some practical aspects which the author has found helpful are described in detail. Some such examples are: linking both professional and other salaries to gross fees as an aid to motivation, cash or credit for accounts receivable, financial analysis to guide money management, and the marketing of pet vaccines.

Economics, Medical↗
PubMedDate not supplied

Educational attainments of school leavers with a preschool history of speech-language impairments.

This paper reports a follow-up study of a cohort of 16- and 17-year-olds with a preschool history of speech-language impairment and whom Bishop and Edmundson (1987) originally studied. Information collected by questionnaire showed that the GCSE grades of those whose language impairments had resolved by 5;06 were below those of age-matched controls. However, the number of GCSE examinations entered and passed was significantly more than those of the 'persistent S-LI' and 'general delay' groups. Overall, IQ was the strongest predictor of educational attainment. However, even when IQ was controlled, literacy skills accounted for independent variance in achievement, especially among those with a history of language difficulty. The survey also noted that the majority of students across all groups remained in full-time education; however, the adolescents with a background of S-LI were more likely to follow vocational and employment training courses rather than A-levels.

Adolescent↗
PubMedDate not supplied

Parents' perceptions regarding the discipline of their hearing-impaired children.

Perceptions that parents have of their hearing-impaired children's misbehaviour, the techniques used in handling these behaviours, and the perceptions of success the parents experience in controlling such actions are examined in this study. One hundred and thirty-four parents with hearing-impaired children responded to a self-report questionnaire eliciting the following information: family background, perceptions of their children's misbehaviours, techniques used in dealing with their children's misbehaviour, perceived success of corrective techniques used, and their beliefs concerning the use of disciplinary techniques. Findings indicate that successful parents (those who perceived themselves as 'successful' in handling their hearing-impaired children's misbehaviour) differed from the unsuccessful parents (those who perceived themselves as 'unsuccessful' in handling their hearing-impaired children's misbehaviour) in two important ways. One was in the way parental groups perceived the incidence of misbehaviour in their children, that is, successful parents reported a significantly lower incidence of misbehaviour than did unsuccessful parents. The second difference involved the types of disciplinary techniques practised to control misbehaviour, that is, successful parents most often chose a technique involving 'discussion with explanation', while unsuccessful parents most often employed techniques involving 'scolding'.

Behavior Therapy↗
PubMedDate not supplied

Expression and in vitro evaluation of rhesus macaque wild type (wt) and modified CC chemokines.

Several human CC chemokines have been shown to inhibit HIV/ SIV infection in vitro, providing the rationale for their potential use in vivo. However, because of their inherent physiological effect, such chemokines are reasoned to be of limited therapeutic value due to potential side effects. The knowledge that amino terminus modified or deleted human RANTES retains its receptor binding properties but loses its signaling properties has provided a means to use such modified chemokines in vivo for possible therapeutic benefits. In efforts to test the efficacy of such modified chemokines, our laboratory has cloned, sequenced, and prepared recombinant forms of wild-type (wt) and amino-terminus modified rhesus macaque chemokines MIP-1alpha, MIP-1beta, and RANTES. These sets of chemokines were tested for their potential to inhibit SIV infection and induce signaling. The data showed that whereas wt chemokines retained both virus inhibitory and signaling functions, corresponding amino-terminus modified chemokines only showed virus inhibitory effects without detectable signaling effects. Such reagents will be valuable for evaluation of their therapeutic potential in vivo, either alone or as adjuncts to other chemotherapeutic drugs.

Amino Acid Sequence↗
PubMedDate not supplied

Recombinant human CD40 ligand inhibits simian immunodeficiency virus replication: a role for interleukin- 16.

CD40 ligand (CD40L), expressed on activated T cells, binds its receptor, CD40, on dendritic cells, B cells, and monocytes/ macrophages. Human immunodeficiency virus (HIV)-infected individuals exhibit normal B-cell CD40 expression but diminished expression of CD40L on CD4 + T cells. Thus, we studied recombinant human CD40L (huCD40L) in an in vitro rhesus macaque model of acquired immunodeficiency syndrome (AIDS). huCD40L induced peripheral blood mononuclear cell (PBMC) proliferation independent of mitogenic cytokines and led to a 70% reduction in p27 production by simian immunodeficiency virus (SIV) mac239 infected PBMCs (P < 0.05). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed reduced expression of SIV gag and increased expression of interleukin (IL)-16 mRNA. Supernatants from huCD40L-stimulated PBMC and control cultures contained similar amounts of IL-16, suggesting an intracellular antiviral effect by IL-16. Phytohemagglutinin (PHA)-stimulated PBMCs similarly cultured with huCD40L showed only slight increases in chemokine production (P > 0.05). These results suggest that huCD40L inhibits replication (antigen and mRNA production) of SIVmac239. This response involves huCD40L induction of IL16 mRNA expression and appears to be independent of beta-chemokines.

Animals↗