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Biomedical subjects

J Vreven

Publications and source records attributed to J Vreven.

At least 37 records · Page 2Linked to original sources
PubMed1985

Antibody specificity and antigen characterization of rat monoclonal antibodies against Streptococcus mutans cell wall-associated protein antigens.

Monoclonal antibodies to Streptococcus mutans OMZ175 (serotype f) cell wall-associated antigens (wall-extracted antigens [WEA]) were derived from the fusion of Lou C plasmocytoma rat cells (IR 983 F) and spleen cells from Wistar R inbred rats immunized with WEA. Four cell lines producing monoclonal antibodies directed against a component of S. mutans WEA have been established. All four monoclonal antibodies reacted only with two antigens of WEA from S. mutans OMZ175 by Western blotting and immunoprecipitation techniques, enzyme-linked immunosorbent assay (ELISA), and competitive ELISA. Western blot analysis of WEA showed that the four monoclonal antibodies recognized two related cell wall-associated proteins with apparent molecular weights of 125,000 and 76,000. Immunoprecipitation of whole cells with the monoclonal antibodies confirmed the surface localization of the two antigens. The ELISA and competitive ELISA were used to analyze the distribution of the epitopes on seven S. mutans serotypes. All S. mutans serotypes were found to express the recognized epitopes; however, different reactivity patterns could be distinguished among the various strains tested, and the four monoclonal antibodies reacted only weakly with S. mutans serotypes d and g.

Animals↗
PubMed1980

[Release of fluoride by fluoridated amalgams and its incorporation by dental enamel].

Liberation of stannous fluoride incorporated in the alloy powder before amalgamation was demonstrated for all the amalgams tested in this study. This liberation was observed for stannous fluoride concentrations of 0.5% and 1%. Clear differences were noted in relation to the type of amalgam studied. Through the use of a non destructive method (activation with charged particles) it was possible to measure the fluoride concentration in enamel before and after the contact with the fluoridated amalgam. This analysis demonstrated that the fluoride liberated from the amalgam was trapped by enamel in contact with alloys containing 0.5% or 1% of stannous fluoride. The enrichment in fluoride was very important in the first weeks and remained high after four months. No significant difference appeared between alloys fluoridated at 0.5% and 1%. The dissolution of calcium fluoride through a KOH treatment showed that the amount of stable fluoride (fluorapatite) formed was important.

Calcium Fluoride↗
PubMed1977

[Dentifrices and dental hygiene].

The best known dentifrices are pastes. The various constituents save to respect integrity of soft and hard tissues. The abrasive must suppress coloration without enamel abrasion. The foaming agent facilitates detachment and elimination of coloration without lesion to the gingiva. Flavours, humectants, binders will not irritate the mucosa. Chlorhexidine and fluoride are among the most studied and interesting therapeutic agents.

Dentifrices↗