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Biomedical subjects

J V Maniglia

Publications and source records attributed to J V Maniglia.

6 recordsLinked to original sources
PubMed2006

LHX6 is a sensitive methylation marker in head and neck carcinomas.

Head and neck cancer remains a morbid and often fatal disease and at the present time few effective molecular markers have been identified. The purpose of the present work was to identify new molecular markers for head and neck squamous cell carcinoma (HNSCC). We applied methylation-sensitive arbitrarily primed PCR (MS/AP-PCR) to isolate sequences differentially methylated in HNSCC. The most frequently hypermethylated fragment we found maps close to a cytosine guanine dinucleotide (CpG) island on chromosome 9q33.2, and hypermethylation of this CpG island was associated with transcriptional silencing of an alternative transcript of the LHX6 gene. Using combined bisulfite restriction analysis (COBRA), hypermethylation of this fragment was detected in 13 of 14 (92.8%) HNSCC cell lines studied and 21 of 32 (65.6%) primary tumors, whereas little or no methylation was seen in 10 normal oral mucosa samples. We extended this investigation to other cancer cell lines and methylation was found in those derived from colon, breast, leukemia and lung, and methylation was also found in 12/14 primary colon tumors. These findings suggest that differentially methylated (DIME)-6 hypermethylation is a good cancer marker in HNSCC as well as in other kinds of neoplasias and confirm the importance of searching for markers of epigenetic dysregulation in cancer.

Base Sequence↗
PubMed2000

Preparation and characterization of collagen-elastin matrices from blood vessels intended as small diameter vascular grafts.

This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen-elastin matrix.

Alkalies↗
PubMed1990

Intraoral-extramaxillary sinus approach for ligation of the maxillary artery: an anatomic study with clinical correlates.

Surgical control of severe epistaxis is usually reserved for cases refractory to more conservative techniques. We present our experience with intraoral ligation of the maxillary artery as it courses through the buccal fat pad before it enters the pterygopalatine fossa and branches posterior to the maxilla. This technique has been found useful in the control of nasal hemorrhage as well as an adjunct to other surgical procedures, such as removal of benign and malignant neoplasms involving the maxilla and paranasal sinuses. This technique was used for ligation of the maxillary artery in 20 patients. The artery was readily identified in some cases, but required more extensive dissection in others; therefore, an anatomic study in 18 preserved and fresh cadaver specimens was undertaken to investigate the variability of the maxillary artery in the region of the buccal fat pad. There appeared to be significant variation in the distance from the buccal mucosal incision site, as well as variation in the relationship to the pterygoid muscles. This relationship to the pterygoids explains the occasional difficulty in locating the artery for ligation. This technique represents a reasonable alternative to the more traditional transantral approach to ligation of the maxillary artery, as long as the surgeon understands the anatomy of the region, its variations, and where the artery may be located if not immediately apparent. No major complications have been experienced.

Adult↗
PubMed1981

Congenital lop ear deformity.

Congenital lop ear is a malformation secondary to an autosomal dominant mendelian factor. This anomaly may be surgically corrected as early as the preschool age. The surgical technique presented is derived from many sources with some personal modifications. It consists of cartilage excision and incision and the use of horizontal mattress sutures. The surgical procedure is organized into six steps. This technique is easy to learn and useful in the training or residents. The complications of otoplasty are discussed.

Adolescent↗
PubMedDate not supplied

[Hearing evaluation in children aged 3-6 years in day-care centers]

OBJECTIVE: To estimate the prevalence of deafness in children 3-6 years old in day-care centers of São José do Rio Preto, SP, Brazil. METHODS: We used free-field audiometry to screen 103 children from 8-selected day-care centers. Children with abnormal examination results were referred to the speech therapy outpatient service to undergo pure-tone conventional audiometry. We adopted the WHO classification for degree of deafness. RESULTS: Audiometric dysfunctions were present in 10 children (9.70%; SD=0.96) with one boy showing left ear mild conductive hearing loss (mean LE=35 dB), while 9 children (8.73%; SD=2.78) showed alterations in air-conduction threshold in 4000, 6000 or 8000 sharp frequencies. Out of 102 children (99.03%; SD=0.96), 55 boys (53.39%; SD=4.9) and 47 girls (45.64%; SD=4.9) presented no hearing loss according to the WHO criteria. CONCLUSIONS: The prevalence of 9.7% audiometric dysfunctions found in this study indicates that deafness prevention programs should be organized.

Journal Article↗