Search PubMed⌕ Search

Biomedical subjects

J V Greiner

Publications and source records attributed to J V Greiner.

99 records · Page 6Linked to original sources
PubMed1978

Number of inflammatory cells in the normal conjunctiva.

We counted inflammatory cells per cubic millimeter in both the epithelium and the substantia propria of samples from upper tarsal conjunctiva of 15 normal subjects and from lower forniceal conjunctiva of ten normal subjects. The upper limit of normal for number of cells was nearly 500,000/mm3. Lymphocytes accounted for about 70% of the inflammatory cells. Neutrophils and lymphocytes were almost always present in both the epithelium and the substantia propria. Plasma cells and mast cells were present in the substantia propria of all subjects, but never in the epithelium. Neither eosinophils nor basophils were found in any specimen. We concluded that conjunctiva of the white and quiet eye usually contains heavy infiltrates of inflammatory cells.

Adolescent↗
PubMed1978

Giant papillary conjunctivitis induced by hard or soft contact lens wear: quantitative histology.

Both hard and soft lens wearers develop a syndrome of decreased tolerance, increased mucus, mild itching, and giant papillary excrescences in the upper tarsal conjunctiva that resemble a varnal conjunctivitis. In the fully developed syndrome, the upper tarsal plate has an increase in stringy mucus and is covered by large papillae crowded together. The syndrome develops after months to years of otherwise successful lens wear and occurs in users of all types of soft and hard lenses. Histologic examination of tissues from 55 patients with well-developed giant papillary conjunctivitis compared with tissue from 15 normal people showed three findings characteristic of the syndrome: (1) mast cells in the epithelium, (2) eosinophils in the epithelium and substantia propria, and (3) basophils in the epithelium and substantia propria. Plasma cells and lymphocytes per cubic millimeter were not increased in detailed counts of 15 patients and 15 normal individuals. It is proposed that the number of plasma cells and lymphocytes cannot increase much beyond the level already present in normal conjuctiva and further influx of mononuclear inflammatory cells is the impetus for growth of the papillae. It is proposed that giant papillary conjunctivitis is a generalized response of the upper tarsal conjunctiva.

Adolescent↗
PubMed1978

Development of the hamster retina: a morphologic study.

Hamster retinas were examined by light and electron microscopy during the first 15 postnatal days and at the adult stage. At birth, these retinas were more primitive than those of other rodents, but eventually they became very similar. Initial postnatal growth and development were more rapid than in other rodents of comparable ages. Mitotic figures were present throughout the first 9 days. The primitive inner plexiform layer formed during days 2 to 4; on day 7, several developmental changes became apparent: outer segment disks of the photoreceptor cells formed from flattened, smooth vesicles, horizontal cells became readily identifiable, the outer plexiform layer appeared, and ribbon synapses formed in the receptor cell axon terminals. By day 11, there were numerous conventional synapses in the inner plexiform layer, and ribbon synapses were beginning to appear in the terminal portions of the bipolar axons. The retinal tissue was progressively invaded by developing vasculature. The first photoreceptor outer segment disks formed from smooth membranous vesicles flattening radially around the ends of the connecting cilium. After the outer segment assumed a more mature appearance, with a stacked disk arrangement, further disk formation occurred by plasma membrane infoldings at the outer segment base. The adult hamster retinas were similar to those of other rodents.

Animals↗
PubMed1977

Giant papillary conjunctivitis in contact lens wearers.

A syndrome that occurred in both hard and soft contact lens wearers was characterized by increased mucus, itching, decreased lens tolerance, and giant papillae in the upper tarsal conjunctiva. It developed in as few as three weeks with soft lens wearers but also occurred after months or even years of successful wear. The histology was characterized by basophils, eosinophils, and mast cells in the epithelium, and these cells as well as increased numbers of lymphocytes, plasma cells, and polymorphonuclear leukocytes in the stroma. The syndrome may be immunologic in origin with deposits on the lenses as the antigen, and the syndrome may be a major cause of difficulty in wearing contact lenses once they have been successfully fit.

Antigens↗
PubMed1977

Phacocryolysis and phacoemulsification.

The effect of rapid freezing and thawing was tested in vitro on 100 human hard nuclear cataracts before ultrasonic emulsification. After phacocryolysis the interspace between lens fibers increased, and the normally closely packed fibers appeared to loosen. This seemed to soften the hard cataract, thereby facilitating ultrasonic emulsification.

Cataract Extraction↗
PubMedDate not supplied

Phosphorus nuclear magnetic resonance and ocular metabolism.

Phosphorus (31P) nuclear magnetic resonance (NMR) represents a noninvasive technique for the assessment of ocular metabolism. The measurement of a spectrum of phosphorus-containing metabolites (e.g., phosphorylated sugars and ATP), including a number of heretofore uncharacterized metabolites, can be made with a single analysis. In addition to quantitating phosphatic metabolites, 31P NMR can be employed to monitor (1) the rate of metabolic change in a specific biochemical reaction via T1 and T2 relaxation times, and (2) the rate of change in the concentration of a particular metabolite. Several calculations indicating tissue energy status (health) can be made using quantitative spectroscopic information including: the phosphorylation potential, the energy charge of the adenylate system, and the 31P spectral modulus. Tissue pH can be determined as a function of shift in 31P NMR signals. 31P NMR techniques have both research and diagnostic applications in ophthalmology since potentially it provides a noninvasive method to analyze ocular tissues metabolically and detect subtle biochemical changes that precede overt manifestation of disease states. Such detection may allow for early and more effective therapeutic intervention of disease. Furthermore, the noninvasive quality of NMR spectroscopy will permit continual evaluation of therapy.

Adenosine Diphosphate↗