Search PubMed⌕ Search

Biomedical subjects

J U Gutterman

Publications and source records attributed to J U Gutterman.

At least 325 records · Page 18Linked to original sources
PubMed1972

Antigen solubilized from human leukemia: lymphocyte stimulation.

Soluble antigen was extracted with hypertonic (3 molar) potassium chloride from the malignant cells of seven patients with acute leukemia. The antigen and leukemia cells were used to stimulate autologous patients' and allogeneic normal donors' lymphocytes in mixed lymphocyte cultures. The lymphocytes of six patients showed significant blastogenic responses to autologous antigen. In contrast, the lymphocytes of only one of seven normal donors responded to the soluble antigens. Both patients' and normal subjects' lymphocytes responded to the intact leukemia cells. The use of these antigens should facilitate the study of specific tumor immunity in human leukemia.

Antigens↗
PubMedDate not supplied

A novel c-abl protein product in Philadelphia-positive acute lymphoblastic leukaemia.

Activation of cellular proto-oncogenes as a result of chromosomal abnormalities has been implicated in the development of some human malignancies. Perhaps one of the most striking examples of this association occurs in chronic myelogenous leukaemia, where the Philadelphia (Ph) translocation results in substitution of the 5' end of the c-abl proto-oncogene with bcr gene sequences. A unique hybrid bcr-abl message is produced. As the Ph translocation is also present in some patients with acute lymphoblastic leukaemia, we initiated studies to determine if similar genomic events occur in these two different forms of Ph-positive leukaemia. Here we report that the Ph translocation in acute lymphoblastic leukaemia can result in production of a novel aberrant c-abl protein that is distinct from the bcr-abl protein found in Ph-positive chronic myelogenous leukaemia. Our observations suggest that alternative mechanisms of activation of c-abl exist, and may be important in the development of human acute lymphoid rather than chronic myeloid malignancies.

Electrophoresis, Agar Gel↗