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Biomedical subjects

J Thurin

Publications and source records attributed to J Thurin.

42 records · Page 3Linked to original sources
PubMed1983

Mass spectrometry of a human tumor glycolipid antigen being defined by mouse monoclonal antibody NS-19-9.

With an antibody-to-chromatogram binding assay to follow the preparation a glycolipid was isolated from human pancreatic carcinoma using a mouse monoclonal antibody of apparent specificity for gastrointestinal tumors. Direct probe mass spectrometry of three derivatives established the sugar sequence as NeuAc-hexose-(fucose)N-acetylhexosamine-hexose-hexose and the ceramide to be composed mainly of phytosphingosine and 16-24 carbon 2-hydroxy fatty acids. NMR spectroscopy of two of the derivatives made likely the presence of the sequence Gal beta 1 leads to 3GlcNAc(4 comes from 1 alpha Fuc)beta 1 leads to, which is the blood group Lewis a determinant. This is in agreement with recent results from degradation studies.

Adenocarcinoma↗
PubMed1980

Glycolipids of rat large intestine. Characterization of a novel blood group B-active tetraglycosylceramide absent from small intestine.

A novel blood group B-active tetraglycosylceramide has been isolated from rat large intestine. It is probably present only in the epithelial cells. Although the compound was not obtained pure, the structure was conclusively established by mass spectrometry, proton NMR spectroscopy and degradative studies to be Galp alpha 1 leads to 3Galp(2 comes from 1 alpha Fucp)beta 1 leads to 4Glcp beta 1 leads to 1Cer. The ceramide was composed of mainly trihydroxy long-chain base (18:0) and non-hydroxy fatty acids (16:0-24:0). The glycolipid was absent from small intestine.

Animals↗
PubMedDate not supplied

Coupling strategies in solid-phase synthesis of glycopeptides.

N-beta-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-asparaginyl peptides [Asn(GlcNAc)] corresponding to T-helper cell determinants were synthesized on solid-phase. Various amino-terminal- and carbohydrate-protecting groups were used on the glycosylated asparagine residue which was coupled to the peptide chain. We found that coupling rates decreased with increased size of the protected carbohydrate part of the acylating agent. Double couplings with an O-unprotected saccharide, as in Fmoc-Asn(GlcNAc)-OH resulted in acceptable coupling rates even with a synthetically difficult sequence corresponding to the T-cell epitopic peptide from the C-terminus of pigeon cytochrome c. The observed coupling rates on this peptide as well as on a T-cell epitopic pentapeptide, derived from the rabies virus N-protein, were comparable to those of conventional solid-phase peptide syntheses. The Fmoc-Asn(GlcNAc)-OH used can be prepared easily from commercially available components. The described glycopeptides will be used to probe effects of N-glycosylation on the immune recognition of viral glycoproteins.

Acetylglucosamine↗