Search PubMed⌕ Search

Biomedical subjects

J Tautz

Publications and source records attributed to J Tautz.

27 records · Page 2Linked to original sources
PubMed1995

Hydrodynamic orientation of crayfish (Procambarus clarkii) to swimming fish prey.

Reversibly blindfolded crayfish (Procambarus clarkii) react to small swimming fish (Astyanax fasciatus mexicanus) approaching or passing nearby with antennal and cheliped movements and body turns (Fig. 3). We studied the accuracy and dynamics of crayfish orientation responses to the previously analyzed hydrodynamic disturbances caused by the fish, mostly produced by tail flicks. Antennal and cheliped movements started slightly before the onset of turning responses (Fig. 4). Antennal sweeps were performed most rapidly. 50% of the appendage sweeps resulted in contacts with the fish (Fig. 5). Most turns were directed toward the stimulus (Fig. 6). Response amplitudes increased with increasing stimulus angle. Turns were accurate for small stimulus angles, but smaller than expected for larger ones. Sweeps of ipsilateral antennae and chelipeds were generally directed backwards, while those of contralateral appendages were smaller and directed forwards. The amplitudes of appendage sweeps first increased with increasing stimulus angle and then decreased again for more caudal stimulus directions. Lateral stimuli (60 degrees-120 degrees) from opposite sides were usually significantly distinguished. The amplitudes of the different elements of orientation behaviour were highly correlated with each other, indicating that they were directed by the same sensory input.

Animals↗
PubMed1994

Comparison of directional selectivity in identified spiking and nonspiking mechanosensory neurons in the crayfish Orconectes limosus.

We have recorded electrical activity from two identified synaptically coupled mechanosensory interneurons in the abdominal nervous system of the crayfish Orconectes limosus and have studied their responses to constant-velocity water-jet stimuli presented from different directions. The two neurons, the ascending caudal photoreceptor (CPR) and the local directionally selective neuron, responded preferentially to stimuli delivered ipsilaterally to their dendritic input regions. Both neurons featured responses consisting of a phasic excitatory "on" response and a tonic depolarizing plateau. The different response components showed various degrees of directional selectivity: The initial "on" peak of the response was the least sensitive and the plateau was the most sensitive to stimulus direction. The CPR showed a sharp cut-off in responsiveness to contralateral stimuli, whereas the local directionally selective neuron showed a more gradual decrease in its directional responsiveness. This difference is a consequence of the feed-forward lateral inhibition that the local directionally selective neuron exerts on the CPR and of the threshold for initiation of action potentials in the CPR. A comparison of the spiking response of the CPR with its generator potential shows that the number and frequency of action potentials are a more sensitive indicator of directional preference than the generator potential response. The directional characteristic of the CPR is discussed as a filter matched to a specific spatial aspect of biologically relevant water movements.

Animals↗
PubMed1991

The time course and frequency content of hydrodynamic events caused by moving fish, frogs, and crustaceans.

In the present study the time course and spectral-amplitude distribution of hydrodynamic flow fields caused by moving fish, frogs, and crustaceans were investigated with the aid of laser-Doppler-anemometry. In the vicinity of a hovering fish sinusoidal water movements can be recorded whose velocity spectra peak below 10 Hz. Single strokes during startle responses or during steady swimming of fish, frogs, and crustaceans cause short-lasting, low-frequency (less than 10 Hz), transient water movements. Low-frequency transients also occur if a frog approaches and passes a velocity-sensitive hydrodynamic sensor. In contrast, transient water movements caused by a rapidly struggling or startled fish or water motions measured in the wake of a slowly swimming (less than or equal to 47 cm/s) trout can be broadbanded, i.e., these water movements can contain frequency components up to at least 100 Hz. High-frequency hydrodynamic events can also be measured behind obstacles submerged in running water. The possible biological advantage of the ability to detect high-frequency hydroynamic events is discussed with respect to the natural occurrence of high frequencies and its potential role in orientation and predator-prey interactions of aquatic animals.

Animals↗
PubMed1988

Accessory pathway for sound transfer in a neotropical frog.

A portion of the lateral body wall overlying the lung cavity of the arboreal frog, Eleutherodactylus coqui, vibrates in response to free-field sound. Peak displacement amplitude of the body wall in response to a natural call note presented at 73 decibels sound pressure level is 1.70 X 10(-9) m, roughly 8 decibels less than that of the ipsilateral eardrum, as measured by laser Doppler vibrometry. We show that the vibration magnitude varies predictably across the body profile and is posture and frequency dependent. Two routes to the inner ear are described for sounds impinging on the body wall; either of these accessory pathways could modify direct input from the peripheral auditory system and enhance sound localization in these small vertebrates.

Animals↗
PubMed1987

Interneurons in the tritocerebrum of the crayfish.

In isolated head preparations of the freshwater crayfish Orconectes limosus 268 local and projecting interneurons with branches in the tritocerebrum have been penetrated with glass microelectrodes and characterized for their sensory inputs. Using 3 criteria (sensory modality, site of receptors, response type of interneurons), the interneurons found were divided into 16 classes. The interneurons were either unimodal mechanoreceptive (89%) or bimodal (9% responding to mechanical and chemical stimuli, 2% responding to mechanical and visual stimuli). No trimodal interneurons were found. Within each modality the neurons received mostly bilateral input (70% of all interneurons responding to antennal stimulation, 84% of all chemosensitive interneurons). If the input was lateralized it was more often ipsilateral. The types of interneuronal responses evoked by sensory stimulation were: neurons that were exclusively excited (84%), those that were exclusively inhibited (10%), those that were excited or inhibited depending on the modality or laterality of the stimulus (6%), those showing long lasting excitatory aftereffects (3%), and those showing excitation or inhibition upon identical stimulation depending on the state of the neurons while being stimulated (1%). Interneurons that responded to mechanical antennal stimulation responded best either to low (10 Hz) or to high (100 Hz) stimulus frequencies. Six neurons responded best to a certain phase relationship between the movements of both antennal flagellae.

Animals↗
PubMed1986

Frequency coding of waterborne vibrations by abdominal mechanosensory interneurons in the crayfish, Procambarus clarkii.

Nine identified interneurons that originate in the 6th abdominal ganglion were studied with intracellular techniques while activating the receptors presynaptic to them with coherent water vibrations of precisely controlled amplitude and frequency. Each of the interneurons showed a characteristic response to different stimulus frequencies that was consistent from animal to animal. As a first approximation, the cells were categorized as low pass, broad band, and high pass interneurons. Two interneurons classified as low pass interneurons (LPIs) have low thresholds to waterborne vibrations below 100 Hz, are inhibited by stimuli above 100 Hz, and respond maximally to 30 Hz stimuli. Three interneurons classified as broad band interneurons (BBIs) respond maximally to stimuli from 30-60 Hz, but also respond well to oscillations as low as 1 Hz and as high as 80 Hz. This class is heterogeneous, spanning the range between low pass and high pass interneurons. Two interneurons classified as high pass interneurons (HPIs) have very high thresholds to water oscillations below 6 Hz. They respond best to 60 Hz oscillations, above which their responsiveness sharply declines, although they continue to respond weakly up to 400 Hz. Two other neurons, also classified as HPIs, responded with relatively few spikes to the stimuli we used. As a result, they do not show a clear peak responsiveness to a particular stimulus frequency.

Animals↗
PubMed1983

Antennal neuropile in the brain of the crayfish: morphology of neurons.

The cellular composition of the antennal neuropile of the crayfish is described. As a context for this work the distribution of neuronal cell bodies throughout the supraoesophageal ganglion (brain) is also described. The neuronal cell bodies in the brain are concentrated in 19 distinct clusters. Three paired clusters are located on the dorsal side of the brain, four paired and one midline cluster bend around the brain laterally and frontally respectively. Fewer than ten somata lie outside of these clusters. The antennal neuropile is composed of primary afferent terminals, efferents, and projecting and local interneurons. The structures of individual neurons of all four types were determined by filling them with Lucifer yellow, and an overview of the neuropile structure was obtained with cobalt backfills of selected nerves. The antennal afferents are concentrated in four main tracts that run medially in the outer layer of the antennal neuropile. Up to 11 orthogonal side branches occur at equal distances (25-35 microns) along the main branches and penetrate the neuropile. The efferents contribute very thin dendrites to the antennal neuropile. The majority of the neuronal mass of the antennal lobe consists of projecting and local interneurons. The branching pattern of the interneurons within the antennal neuropile also shows an orthogonal arrangement of main branches and higher-order branches. Thus the antennal neuropile displays a strong geometrical regularity: Main processes of all four types of neurons run in bundles the length of the long axis of the neuropile (lateral to medial inside the brain) giving rise to orthogonal side branches at regular intervals. This branching pattern leads to a striped appearance of the antennal lobe.

Animals↗
PubMed1980

Ultrastructure and mechanical properties of an insect mechanoreceptor: stimulus-transmitting structures and sensory apparatus of the cercal filiform hairs of Gryllus.

1. The following features of the cercal filiform hairs of the cricket Gryllus were investigated: (a) the ultrastructure and geometrical peculiarities of the various auxiliary structures in the region of the hair base, as well as those of (b) the stimulus-receiving outer segment of the dendrite (including the tubular body), and (c) the mechanical properties (directionality and linearity and frequency dependence of mobility) of the hair. 2. When stimulated by vibrations of the medium, the filiform hairs show regular or irregular oscillations depending on stimulus intensity. At higher stimulus intensities (xi > congruent to 100 microns at 100 Hz) the hairs flutter irregularly in various directions, at somewhat lower intensities preferentially in the plane of best mobility in even lesser intensities in the plane of stimulus vector. In the plane ob best mobility the maximal angle of deflection from the resting position is 5.3 +/- 1.4 degrees. 3. The dependence of hair mobility on stimulus frequency was tested in the range 20-1000 Hz. Best mobility was found in the range 100-200 Hz. 4. The directional characteristic of hair mobility has the form of a figure eight. Hairs can be grouped into three classes on the basis of direction (with respect to the long axis of the cercus) of best mobility: parallel (L-hairs), transverse (T-hairs), and diagonal (D-hairs). 5. The plane of best mobility corresponds with the plane symmetry of the hair base. The hair can be deflected furthest from the resting position in the direction of a cuticular peg at the hair base, which projects toward the lumen of the hair and marks the flat side of the tubular body within the terminal dendrite segment. Deflection of the hair shaft in the opposite direction is limited by a fibrous cushion, which exerts a counter-pressure. When the hair is deflected, the cuticular peg causes deformation of the tubular body. 6. The direction of best mobility of the hair is the direction in which the sensory cell is depolarized; the direction of depolarization can thus be determined entirely by morphological criteria.

Animals↗