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Biomedical subjects

J Takeuchi

Publications and source records attributed to J Takeuchi.

At least 253 records · Page 14Linked to original sources
PubMed1983

Choroid plexus papilloma. Light and electron microscopic study.

Choroid plexus papilloma (CPP) was observed by light and electron microscopy, using surgically excised tissues in 7 cases and cultivated cells. CPP cells had numerous microvillous processes showing balloon-like features, lysosomes containing haemosiderin, and highly electron-dense irregular granules about 150-300 nm in diameter. Interstitial cells with highly electron-dense cytoplasm inserted their long and thin processes into the invagination of basal plasmalemma of CPP cells, occasionally breaking down the basal lamina. Many of them were located in the intercellular space among CPP cells, sometimes adhering to the ventricular surface of CPP cells. Ruthenium red stain was positive on the surface of CPP cells and was especially intense on the surface of microvilli and cilia. In culture, CPP cells and interstitial cells migrating from the CPP cell mass showed a phagocytic activity after treatment with Latex.

Adult↗
PubMed1983

Malignant papillary cystadenoma lymphomatosum. Light and electron microscopic study.

A case of malignant papillary cystadenoma lymphomatosum was investigated by light and electron microscopy. Histologically, the tumor consisted mainly of a characteristic papillary cystadenoma lymphomatosum, but the feature of epithelial components varied greatly in separate portions of the tissue; papillary adenoma-, tubular adenocarcinoma-, acinic cell tumor- or oncocytoma-like features were observed, the tumor cells showing "atypia" in major components. Numerous intracytoplasmic inclusions were found in many oncocytic cells arranged in acinar structure, and stained with PAS, AZAN or Alcian blue. Electron microscopically, large numbers of very large, swollen and closely packed mitochondria were observed in almost all the tumor cells. The intraplasmic inclusions were composed mainly of a long dense body enclosed by a single unit membrane, and the contents constituted bundles of fine filament running parallel to the longer axis. The inclusions were often observed to unite with lysosomes and to co-exist with secretory granules in the cytoplasm.

Adenolymphoma↗
PubMed1983

Distribution of S-100b protein in normal salivary glands and salivary gland tumors.

Immunohistochemical studies were performed for the presence of S-100b protein in non-neoplastic and neoplastic salivary gland tissues by the peroxidase anti-peroxidase (PAP) method. Some cases of pleomorphic adenoma were investigated by immuno-electron microscopy. S-100b protein could not be detected in epithelial cells of intercalated ducts, acini, striated ducts and excretory ducts of non-neoplastic salivary gland. However, myoepithelial cells surrounding the acini and intercalated ducts were specifically stained by S-100b protein. In pleomorphic adenomas, S-100b protein-positive cells could be mostly observed in the myxoid and chondroid areas, and the basal layer cells of the double-layered ductal cells were also positive. In clear cell adenoma, the clear cells were also S-100b protein positive. In adenoid cystic carcinomas, S-100b protein-positive cells could be found in trabecular areas, but not in tumor cells showing cribriform-pattern. In other tumors (Warthin's tumor, oxyphilic adenoma, basal cell adenoma, mucoepidermoid tumor and acinar cell carcinoma), S-100b protein positive cells were seldom observed. Immuno-electron microscopically, S-100b protein was diffusely distributed in the cytoplasm of myoepithelial cells as well as of tumor cells of pleomorphic adenoma, being distributed especially on the membrane of endoplasmic reticulum and the outer nuclear membrane.

Adenoma, Pleomorphic↗
PubMed1983

Case report of neuroendocrine carcinoma of the skin, histochemical and electron microscopic study.

A rare case of neuroendocrine carcinoma of the skin in a 83-year-old male Japanese was reported. Histological, electron microscopical and cytological studies were performed on the surgically removed tumor tissue and the cultured tissue. The tumor occurred at the junction of the dermis and subcutaneous tissue of the right elbow, and spread to the right brachial region and axilla. Histologically, the tumor consisted mostly of small anaplastic cells, closely resembling lymphocytes. They showed a characteristic uniformity without rosette or trabecular formation in the usual histological sections, but an epithelial-like arrangement of tumor cells was readily observable both in the tissue culture and imprint preparations. Grimelius' stain was weakly positive in the periphery of the cytoplasm. Neuron Specific Enolase (NSE) activity was high both in the serum and in the tumor mass. No gastro-entero-pancreatic hormones were detected. Electron microscopically, membrane-bound granules of neurosecretory type, 90 to 170 nm in diameter were observed in the cytoplasm. These granules were characteristically distributed along the periphery of cytoplasm beneath the plasma membrane and in clusters in the cell processes. The tumor cells had sparse rudimentary desmosome-like junctions and a few cytoplasmic finger-like projections. Either a neurogenic or APUD cell origin of the tumor was suspected. The serum NSE value, suggesting to be a neurogenic origin before the histological examination in the present case, is considered to be a valuable marker substance for screening and therapeutic monitoring of neurogenic tumors.

Aged↗
PubMed1983

Influence of fixed fibroblasts on glycosaminoglycan synthesis of human gastric carcinoma cells in vitro.

The influence of fixed fibroblasts on the glycosaminoglycan (GAG) synthesis of gastric carcinoma cells was examined by incubation along with [3H]glucosamine. In well-differentiated adenocarcinoma cells, the amount of 3H-GAG in the interface material between the carcinoma cells and the fixed fibroblasts was much larger (about twenty times) than in the interface between the carcinoma cells and the bare culture plates, and 3H-GAG consisted mainly of heparan sulfate, with a small amount of dermatan sulfate and chondroitin sulfate. On the other hand, in poorly differentiated carcinoma cells, the amount of 3H-GAG in the interface material produced by the carcinoma cells on the fibroblast was almost the same as on the bare culture dish. In a conventional monolayer culture, well-differentiated adenocarcinoma cells produced a much greater amount of GAG, consisting mainly of dermatan sulfate, chondroitin sulfate and heparan sulfate, than poorly differentiated carcinoma cells. Almost the same amount of hyaluronic acid was secreted into the medium by both types of carcinoma cells.

Adenocarcinoma↗
PubMed1983

Rat pituitary adenoma and hyperplasia induced by caffeine administration.

The effect of caffeine by oral administration on female Wistar rats was studied for 12 months. High concentrations (2000 micrograms/mL) of caffeine caused a decrease in body weight and an increase in the weight of the pituitary gland in these rats. Increased pituitary weight was caused by the growth of a pituitary adenoma or hyperplasia. Pituitary adenomas and instances of hyperplasia were found in 27 of the 40 rats in the caffeine group, compared to 9 of the 30 rats in the control group. The histologic classification used was microadenoma, papillary (or sinusoidal) macroadenoma, and diffuse macroadenomas. These adenomas appeared to be endocrinologically nonfunctioning.

Adenoma↗
PubMed1983

Choriocarcinoma arising from the pituitary fossa with extracranial metastasis: a review of the literature.

Since Askanazy's first report on a case of primary intracranial choriocarcinoma in 1906, more than 60 cases have been reported so far. We add to that a case of intrasellar choriocarcinoma with suprasellar extension: A 12-year-old girl showed cranial nerve palsies, hypopituitarism, and abnormally high titers of human chorionic gonadotropin (HCG) in blood and in cerebrospinal fluid (CSF). She died 3 months after surgery. At autopsy, an intra- and suprasellar choriocarcinoma, which penetrated through the clivus into the cerebellopontine angle and metastasized remotely to the lungs, was confirmed. This rare case is presented with a thorough review of the literature.

Adolescent↗
PubMed1983

Possible specific chromosome changes in large bowel cancer.

Structural and numerical changes affecting chromosomes number 7 and number 12 were the most frequent karyotypic changes observed in 10 large bowel cancers. The findings are briefly discussed in relation to the development of this malignancy.

Adenocarcinoma↗
PubMed1983

Kappa and lambda immunoglobulin expression associated with abnormalities of chromosomes #2 and #22 in lymphoma and leukemia.

The types of surface immunoglobulins on Burkitt lymphoma (BL) cells correlate with the specific chromosomes that are altered in the tumor. For example, BL with a t(2;8) translocation expresses kappa (kappa) light chains, whereas BL with a t(8;22) translocation expresses lambda (lambda) light immunoglobulin chains; these correspond with the locations of the kappa chain genes on chromosome #2 and the lambda chain genes on chromosome #22, respectively (1-5). In order to explain this close correlation between specific translocations and light chain expression in BL and BL-type acute lymphocytic leukemia (ALL) (L3 type), Hecht et al. [6] proposed the concept of position effect, which is reviewed herein.

Adolescent↗
PubMed1983

Epidermoid carcinoma arising in parotid adenolymphomatous lesion with microdeposit of amyloid substance.

A case of an epidermoid carcinoma which arose in a cystic adenolymphoma-like lesion of the parotid gland is presented. Histologically, in the major part, the tumor tissue showed a papillary cystadenoma lymphomatosum-like pattern with the stroma consisting of mainly plasma cells and lymphocytes, but the epithelial element was mostly multilayered, showing a keratinous differentiation. In some areas, sebaceous-like cells and oxyphilic cells were found in the epithelial component. In the surrounding fibrous tissue, the invasive growth of the epithelial cells could be observed. Microdeposit of amyloid substance was found in the lymphoid stromal tissue and also in the intercellular space of the epithelial element. A mass of epithelioid cells and cholesterol crystal clefts were observed in the granulomatous tissue which proliferated around the tumor tissues. About 4 months after surgical excision, a recurrent tumor, which was well differentiated squamous cell carcinoma, appeared on the same site as the primary tumor.

Adenolymphoma↗
PubMed1983

Increased urinary kallikrein-like activity in the syndrome of inappropriate secretion of antidiuretic hormone.

The role of the kallikrein system in the natriuresis of SIADH was studied in 3 patients. Following free water intake, serum Na decreased. Urinary Na excretion, urinary aldosterone excretion and urinary kallikrein-like activity (UKaV) increased. Significant relationships were observed between UKaV and urinary aldosterone excretion during the study. Further, there were significant relationships between serum Na and UKaV, and between uric acid clearance and UKaV. In 1 patient, who was observed for a longer period, a significant relationship was observed between Na clearance and UKaV. We conclude that UKaV is increased during hyponatremia in SIADH by volume expansion. This increase in UKaV may play a role in the natriuresis in SIADH.

Aged↗
PubMed1983

Unusual monoclonal DNA binding immunoglobulin.

The monoclonal antibodies directed against DNA were produced by somatic cell hybridization with parental cells (SP-2) and spleen cells from nonimmunized autoimmune MRL/lpr mice. The immunoglobulins were recovered from the culture supernatant from hybridoma by a solid immunoadsorbent and antibody immunoprecipitation. The results from the specificities of DNA binding monoclonal immunoglobulins suggest that the antibodies to DNA have the antibody combining sites for both epitope of double stranded helix and base of DNA and support the concept of the multiple antigen binding potentials of the hybridoma autoantibodies.

Animals↗
PubMed1983

Sulbenicillin-induced kaliuresis in man.

The mechanism of kaliuresis induced by massive antibiotic administration was studied using alpha-sulfobenzyl penicillin (SBPC). In experimental group (n = 8), urinary electrolytes excretion were compared between following the infusion of 10 g SBPC in 200 ml water at a constant rate and following the infusion of 48 mmol of NaCl (equal to that contained in 10 g SBPC) in 200 ml water. For the control group, 96 mmol NaCl in 400 ml water was infused (n = 5). In the experimental group, urinary Na (UNaV) and urinary K excretion (UKV) increased relative to the control period. In the control group, UKV was not increased although UNaV was increased (p less than 0.05). UKV following SBPC infusion was correlated with UNaV (p less than 0.05) and urinary SBPC excretion (p less than 0.05). The ratio of urinary anion gap to urinary cation [1-(urinary Cl concentration/(urinary Na concentration + urinary K concentration))] was significantly increased following SBPC infusion (p less than 0.005) but not in the control group. This increase in anion gap is possibly due to urinary SBPC, which will be ionized over 90% as nonreabsorbable anion in maximally acidic urine. We conclude that the kaliuresis induced by massive SBPC administration in man is probably caused by the nonreabsorbable anion effect of SBPC itself.

Acid-Base Equilibrium↗
PubMed1983

Clonal chromosome abnormalities in patients with Waldenström's and CLL-associated macroglobulinemia: significance of trisomy 12.

We performed cytogenetic analyses by Q- and G-banding techniques of unstimulated or B-mitogen-stimulated spleen, bone marrow, and peripheral blood cells from six patients with malignant macroglobulinemia [two with Waldenström's macroglobulinemia (WM) and four with chronic lymphocytic leukemia associated macroglobulinemia (CLL-M)]. Normal karyotypes were obtained in two of the treated patients (one with WM in remission and the other with CLL-M in relapse). An extra chromosome 12 (trisomy 12) was observed in all four untreated patients. In patient no. 2 (K.R.) and no. 3 (F.G.) with CLL-M, an abnormal karyotype, with trisomy 12 as the only abnormality, was identified. In patient no. 1 (C.C.) with WM, there were two clonal chromosome changes, identified: 47, XX, -9, +12, plus marker chromosome and 48, XX, -9, +12, plus both marker and minute chromosomes. In patient no. 4 (R.M.) with CLL-M, a minute chromosome with or without loss of a G-group chromosome was seen in some metaphases without trisomy 12, in addition to metaphases with trisomy 12 alone. Each of the four untreated patients with WM or CLL-M had clonal chromosome abnormalities, suggesting that chromosome changes may be more frequently associated with WM or CLL-M than with typical CLL without macroglobulinemia. These observations also suggest that trisomy 12 may be the primary karyotypic change in malignant macroglobulinemia, whereas the appearance of the minute or marker chromosome as well as the loss of G-group chromosomes or chromosome no. 9 may be secondary karyotypic changes resulting from clonal evolution in these malignancies.

Aged↗
PubMed1983

Lymphocyte cytotoxicity for autologous human hepatocytes in alcoholic liver disease.

Alcoholic liver disease has been shown to progress even after cessation of ethanol intake and the involvement of an immunological mechanism has been suggested. To study whether lymphocyte cytotoxicity for autologous human hepatocytes is involved in the pathogenic process of alcoholic liver disease, hepatocytes (target cells) obtained by a needle liver biopsy from 36 patients with alcoholic liver disease were isolated by enzymatic digestion and incubated with autologous peripheral lymphocytes (effector cells). Using a microcytotoxicity assay, a cytotoxic effect was observed in patients with active cirrhosis or alcoholic hepatitis, but not in those with inactive cirrhosis, hepatic fibrosis or fatty liver. When lymphocytes were separated into T cell enriched and non-T cell enriched fractions, this cytotoxic effect was significantly greater with the non-T cell enriched lymphocyte fraction than with the T cell enriched fraction. The addition of aggregated IgG reduced the cytotoxic effect of the lymphocytes. These results suggested that antibody-dependent cell-mediated cytotoxicity may be of pathogenic importance in alcoholic liver disease.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1983

[Meningioma of the sphenoid ridge recurring into the pterygomaxillary fossa following craniotomy. A case report and surgical approach].

Meningiomas sometimes invade into the surrounding structures, especially into the sinuses, and show remote extracranial metastasis. Meningiomas of the sphenoid ridge sometimes extend and invade into the cavernous sinus, diaphragma sellae, sphenoid and ethmoid sinuses, orbit and the pterygomaxillary fossa. A case of the tumor of the right sphenoid ridge, which recurred into the orbit and the pterygomaxillary fossa two years after the total removal, was presented. Histological examinations of both the first and second tumor showed meningotheliomatous meningioma. A 61-year-old female was admitted on January 17, 1980 with a complaint of left hemiparesis, memory disturbance, disorientation and incontinentia urinae. Right carotid angiography and CT scan showed a large high density mass in the right middle cranial fossa, which was totally removed. After the operation, the neurological status rapidly improved and she spent an uneventful life until February 1982 when she noticed right exophthalmos, ophthalmoplegia and visual loss. Right CAG and CT scan at the second admission showed a strongly enhanced mass in the right orbit and pterygomaxillary fossa. Severe destruction of the posterolateral wall of the right orbit and the floor of the right middle cranial fossa was also noticed. The tumor was totally removed, using modified Dieffenbach-Weber-Fergusson approach, which is usually used for the carcinoma of the maxillary sinus. By this approach, we could easily reach the pterygomaxillary fossa, that is, the floor of the middle cranial fossa and the posterolateral wall of the orbit. This approach seemed to be very useful for the removal of the tumors of the skull base.

Craniotomy↗