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Biomedical subjects

J Tachi

Publications and source records attributed to J Tachi.

23 records · Page 2Linked to original sources
PubMed1987

Separation and analysis of mononuclear cells infiltrating the thyroid of patients with Graves' disease.

A simple method was established for separating lymphocytes infiltrating the thyroid from thyroid epithelial cells. Namely, suspensions of minced thyroid from patients with Graves' disease were layered on a Percoll two-step density gradient (p = 1.050 and 1.077 g/ml) and centrifuged (400g, 30 min, 4 degrees C). In this way 0.1-18 X 10(5) lymphocytes/g of thyroid tissue with a purity of 65-95% were obtained. Thyroid lymphocytes were analyzed quantitatively with monoclonal antibodies by laser flow cytometry and compared with peripheral lymphocytes. The proportion of OKT3+ cells was decreased with increase in OKIa+ cells. The percentage of OKIa+ cells was significantly correlated with that of Leu12+ cells. The percentages of OKT4+ cells and OKIa+ cells were higher when analyzed with an extended gate window, which was arranged for detection of activated, large-sized lymphocytes. The percentages of OKT8+ and Leu7+ cells were not significantly different from those in peripheral blood. From these results it was concluded that the proportion of B lymphocytes is increased and that of T lymphocytes is decreased, the proportion of activated B lymphocytes is increased, some helper/inducer T cells are activated in the thyroid gland in Graves' disease, and these activated lymphocytes may be important in local production of antithyroid autoantibodies.

Adult↗
PubMed1986

Radioreceptor assay of anti-TSH receptor antibody activity: comparison of assays using unextracted serum and immunoglobulin fractions, and standardization of expression of activities.

A radioreceptor assay for TSH receptor antibodies developed by Shewring and Smith is described in which unextracted serum is used. The assay is simple and reproducible. TSH-binding inhibitor immunoglobulin (TBII) activity determined using unextracted serum correlated well with that determined using the immunoglobulin fraction purified with polyethylene glycol. The assay detected TSH receptor antibodies in 81 of 85 patients (95%) with untreated Graves' disease, no patients with Graves' disease in remission, and 7 of 57 patients (12%) with Hashimoto's disease. TSH-binding inhibitor activity of a human TSH preparation (Kabi Diagnostica) was not detectable at 100 mU/L, but detectable at 1,000 mU/L. Consistent with this, TBII activity was not detectable in hypothyroid patients with goitrous Hashimoto's disease, whose serum TSH concentrations were 11-520 mU/L. Of 57 patients with Hashimoto's disease positive TBII activity was detected in only 7 of 15 patients (46%) with primary atrophic hypothyroidism. Five of these had very high TBII activities (greater than 90% inhibition of labelled TSH binding), and no linear dose-response relationship was observed in dilution experiments. For exact determination of TBII activity in specimens with high activities, serum samples were diluted with normal pooled serum and activities were expressed in U/ml by comparison with values for standard serum, prepared from bovine TSH diluted with normal pooled serum. In this way, samples with TBII activities of 1-50 U/ml (20-90% inhibition of binding of labelled TSH) showed linear dose-response relations in dilution experiments, and the dilution curves of all samples examined were parallel to the standard curve. This standardization procedure was used in determining the half life of TBII.

Autoantibodies↗
PubMed1985

Highly sensitive immunoenzymometric assay for human thyrotropin.

A sensitive assay procedure for immunoenzymometric assay of serum thyrotropin (TSH) was developed by making several modifications of the Enzymun-Test TSH kit (Boehringer, Mannheim GmbH). Serum samples were first incubated in plastic tubes precoated with monoclonal antibodies specific to the beta subunit of human TSH. After the tubes were washed, the TSH bound to the tubes was detected with peroxidase-conjugated polyclonal antibodies to TSH. The sensitivity of the assay was 0.2 milli-int. unit/L, and the intra-and interassay CVs were less than 10%. Analytical recovery was 96 to 106%. The normal basal range of TSH was 0.5 to 4.8 milli-int. units/L. The basal levels of TSH in all but one of 48 thyrotoxic patients with Graves' disease were less than 0.2 milli-int. unit/L, clearly different from those of normal subjects. Thyrotoxic patients in early normal pregnancy showed TSH concentrations of 1.7 to 2.9 milli-int. units/L by conventional double-antibody radioimmunoassay, possibly from cross reactivity with human choriogonadotropin, but undetectable TSH by this method. Measurement of basal TSH by this sensitive assay can be used as an initial screening test for thyroid dysfunction.

Adult↗