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Biomedical subjects

J Tóth

Publications and source records attributed to J Tóth.

185 records · Page 11Linked to original sources
PubMedDate not supplied

Immunohistochemistry and reverse transcriptase polymerase chain reaction on sentinel lymph nodes can improve the accuracy of nodal staging in breast cancer patients.

In this study the nodal staging sensitivity of sentinel lymph node biopsy (SLNB) with detailed pathological and molecular biological examination has been investigated and compared to that of axillary lymph node dissection (ALND) with routine histological evaluation. Sentinel lymph nodes (SLNs) were removed by the dual-agent injection technique in 68 patients with primary, clinically node-negative breast cancer. Forty-seven patients had negative SLNs according to hematoxylin and eosin (H&E) staining. These H&E-negative SLNs were serially sectioned and examined at 250 microm levels by anticytokeratin immunohistochemistry (IHC). In 14 patients the SLNs were also investigated by cytokeratin 20 (CK20) reverse transcriptase polymerase chain reaction (RT-PCR). SLNB with IHC increased the node-positive rate by 26% (by 40% in tumors less than or equal to 2 cm in size (pT1) and by 9% in tumors more than 2 cm but less than or equal to 5 cm in size (pT2)). The sensitivity of SLNB with IHC was superior to that of ALND with routine histology in pT1 tumors and identical in pT2 tumors. The concordance between histology and RT-PCR was only 21%, and in two of three cases with positive histological results RT-PCR was negative. In conclusion, SLNB with detailed pathological and/or molecular biological evaluation can improve the sensitivity of regional staging. ALND can probably be abandoned in patients with pT1 SLN-negative breast cancer. Further prospective studies are required to determine the clinical significance of these detailed SLN evaluation techniques, but at present these methods are still investigational.

Adult↗
PubMedDate not supplied

Correlation of biochemical tumor markers with conventional pathological features in primary breast cancer.

In this prospective study the correlation of pathological with biological prognostic factors and serum tumor markers has been investigated in 574 patients with primary invasive breast cancer. The p53 protein and Bax level correlated positively with tumor size, lymph node status and histological grade. The serum levels of CEA, CA 15.3, TPA-M and TK correlated with tumor extent. There was a significant difference between pre- and postmenopausal breast cancer patients in serum levels of TPA-M and cytosol levels of Bax. Whether these correlations can help in predicting the prognosis of breast cancer by providing additional information with respect to the conventional factors, will have to be investigated by several years of careful clinical follow-up.

Biomarkers, Tumor↗
PubMedDate not supplied

Phenotypic alterations of 7,12-dimethylbenz(a)anthracene and N-methyl-N-nitrosourea induced rat mammary tumors during repeated transplantations.

New transplantable rat mammary tumor lines derived from 7,12-dimethylbenz(a)anthracence (DMBA)- and N-methyl-N-nitrosourea (MNU)-induced carcinogenesis were established and characterized for biological and morphological criteria in the course of multiple tumor generations growing in female or male Wistar rats. DMBA-derived tumors RMT-1, RMT-2 and RMT-3 converted from adenocarcinoma to fibrosarcoma in the early passages. Conversion proceeded earlier in tumors with a well differentiated epithelial component compared to less differentiated ones, providing evidence for clonal spindle cell selection as the most probable mechanism responsible. On the other hand, MNU-derived tumor lines--RMT-4 and RMT-5--maintained histological patterns of parent tumors for a long time, exceeding 20 passages. Analysis of stromal cell tumor component and its interference with developmental sequences of transplantable tumor progression contributes to the explanation of some recent divergent findings (1, 2, 3, 4, 5).

9,10-Dimethyl-1,2-benzanthracene↗